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公开(公告)号：EP2022855B1
公开(公告)日：2015-08-19
申请号：EP07743912.3
申请日：2007-05-16
发明人： CHIBA, Yasunori , JIGAMI, Yoshifumi , KURODA, Kosuke , KOBAYASHI, Kazuo , ICHIKAWA, Kimihisa , NONAKA, Koichi , SUZUKI, Takeshi
CPC分类号： C07K16/2875 , C07K16/00
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公开(公告)号：EP2022855A1
公开(公告)日：2009-02-11
申请号：EP07743912.3
申请日：2007-05-16
发明人： CHIBA, Yasunori , JIGAMI, Yoshifumi , KURODA, Kosuke , KOBAYASHI, Kazuo , ICHIKAWA, Kimihisa , NONAKA, Koichi , SUZUKI, Takeshi
CPC分类号： C07K16/2875 , C07K16/00
摘要： This invention provides a means for enabling high-level secretory production of proteins, in particular proteins having complicated structures such as antibodies, in host cells such as yeast cells. The invention also provides transformed yeast cells having the activated HAC1 gene and the RRBP1 gene and a method for enabling high-level secretory production of foreign proteins using such transformed host cells by inhibiting O-sugar chain formation indigenous to host cells such as yeast cells.
摘要翻译： 本发明提供了用于在宿主细胞如酵母细胞中高水平分泌性生产蛋白质，特别是具有复杂结构如抗体的蛋白质的手段。 本发明还提供具有活化的HAC1基因和RRBP1基因的转化酵母细胞，以及通过抑制宿主细胞如酵母细胞固有的O-糖链形成，使用这种转化的宿主细胞能够高水平分泌生产外源蛋白质的方法。
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公开(公告)号：EP3521428A1
公开(公告)日：2019-08-07
申请号：EP17858330.8
申请日：2017-10-02
发明人： MASUDA, Kenji , NONAKA, Koichi , TANEMURA, Hiroki
摘要： The present invention provides an approach to enhancing the production of a foreign protein serving as a protein-based pharmaceutical product in host cells such as cultured cells derived from a mammal. The present invention provides transformed cells having a novel Hspa5 gene promoter, and a method for secreting and producing a foreign protein at high levels using the transformed host cells.
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4.发明公开
公开(公告)号：EP2210940A1
公开(公告)日：2010-07-28
申请号：EP08845087.9
申请日：2008-10-29
发明人： CHIBA, Yasunori , JIGAMI, Yoshifumi , TAKAHASHI, Yoshie , KURODA, Kosuke , KOBAYASHI, Kazuo , ICHIKAWA, Kimihisa , NONAKA, Koichi , SUZUKI, Takeshi , ONO, Minako
CPC分类号： C12P21/02 , C07K14/39 , C07K14/395 , C07K14/47 , C07K16/00 , C12N9/0051 , C12N9/1048 , C12N9/1051 , C12N9/14 , C12N9/90 , C12N15/67
摘要： This invention provides a means for high-level secretory production of a protein, and, in particular, a protein having a complicated structure such as an antibody, in a host cell such as a yeast cell. This invention provides a method for high-level secretory production of a foreign protein with the use of a transformed host cell having one or more types of chaperone protein genes and via suppression of O sugar chain inherent to a host cell such as a yeast cell.
摘要翻译： 本发明提供了在宿主细胞如酵母细胞中高蛋白分泌生产蛋白质，特别是具有复杂结构如蛋白质的蛋白质的方法。 本发明提供了使用具有一种或多种伴侣蛋白基因的转化宿主细胞并通过抑制宿主细胞如酵母细胞固有的O糖链来高效分泌外源蛋白的方法。
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公开(公告)号：EP2210940B2
公开(公告)日：2018-07-18
申请号：EP08845087.9
申请日：2008-10-29
发明人： CHIBA, Yasunori , JIGAMI, Yoshifumi , TAKAHASHI, Yoshie , KURODA, Kosuke , KOBAYASHI, Kazuo , ICHIKAWA, Kimihisa , NONAKA, Koichi , SUZUKI, Takeshi , ONO, Minako
IPC分类号： C07K14/39 , C07K14/395 , C07K16/00 , C12N15/67 , C07K14/47 , C12N9/02 , C12N9/10 , C12N9/14 , C12N9/90 , C12P21/02
CPC分类号： C12P21/02 , C07K14/39 , C07K14/395 , C07K14/47 , C07K16/00 , C12N9/0051 , C12N9/1048 , C12N9/1051 , C12N9/14 , C12N9/90 , C12N15/67
摘要： This invention provides a means for high-level secretory production of a protein, and, in particular, a protein having a complicated structure such as an antibody, in a host cell such as a yeast cell. This invention provides a method for high-level secretory production of a foreign protein with the use of a transformed host cell having one or more types of chaperone protein genes and via suppression of O sugar chain inherent to a host cell such as a yeast cell.
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公开(公告)号：EP3196304A1
公开(公告)日：2017-07-26
申请号：EP15826531.4
申请日：2015-07-29
发明人： NONAKA, Koichi , SUZUKI, Takeshi , TSUDA, Masashi , BABA, Satoshi , ICHIKAWA, Kimihisa , CHIBA, Yasunori , YOKO-O, Takehiko , ITO, Rie
CPC分类号： C12N15/113 , C12N15/09 , C12N15/62 , C12N15/81 , C12P21/02
摘要： The object of the present invention is to provide a production system that is capable of high-level secretory production of a protein (and in particular, a protein with a complicated structure such as a structure with S-S bonds) in a host cell such as yeast and is suitable for industrial production with high safety that does not require explosion-proof facilities. The present invention provides a transformed yeast into which a chaperone gene has been introduced and in which the aox1 gene and/or the protease gene have been disrupted and a method for producing a protein involving the use of such transformed yeast.
摘要翻译： 本发明的目的在于提供一种能够在酵母等宿主细胞中高水平分泌生产蛋白质（特别是具有SS键的结构等复杂结构的蛋白质）的生产系统 适用于不需要防爆设施的安全性高的工业生产。 本发明提供导入了伴侣基因并且其中aox1基因和/或蛋白酶基因已被破坏的转化酵母，以及涉及使用这种转化酵母的蛋白质生产方法。
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公开(公告)号：EP2210940B1
公开(公告)日：2015-03-11
申请号：EP08845087.9
申请日：2008-10-29
发明人： CHIBA, Yasunori , JIGAMI, Yoshifumi , TAKAHASHI, Yoshie , KURODA, Kosuke , KOBAYASHI, Kazuo , ICHIKAWA, Kimihisa , NONAKA, Koichi , SUZUKI, Takeshi , ONO, Minako
IPC分类号： C07K14/39 , C07K14/395 , C07K16/00 , C12N15/67 , C07K14/47 , C12N9/02 , C12N9/10 , C12N9/14 , C12N9/90 , C12P21/02
CPC分类号： C12P21/02 , C07K14/39 , C07K14/395 , C07K14/47 , C07K16/00 , C12N9/0051 , C12N9/1048 , C12N9/1051 , C12N9/14 , C12N9/90 , C12N15/67
摘要： This invention provides a means for high-level secretory production of a protein, and, in particular, a protein having a complicated structure such as an antibody, in a host cell such as a yeast cell. This invention provides a method for high-level secretory production of a foreign protein with the use of a transformed host cell having one or more types of chaperone protein genes and via suppression of O sugar chain inherent to a host cell such as a yeast cell.
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