公开(公告)号：EP2831259A1

公开(公告)日：2015-02-04

申请号：EP13712051.5

申请日：2013-03-12

申请人： Danisco US Inc.

发明人： KLEINHOUT, T. ,  KOOPS, Bart Christiaan ,  KOOY, Floor K. ,  LEE, Sung Ho ,  SHETTY, Jayarama K. ,  STROHM, Bruce A.

IPC分类号： C12P19/14 ,  C13K7/00

CPC分类号： C12P19/12 ,  A23L29/35 ,  C12P19/14 ,  C12Y302/01001 ,  C12Y302/01002 ,  C12Y302/01068 ,  C12Y302/01133 ,  C13K7/00

摘要： The present teachings provide direct conversion of granular starch into a soluble sugar composition comprising a high, very high, and/or ultra high maltose content. The method involves contacting an aqueous slurry of granular starch with an enzyme composition comprising an appropriate ratio of an alpha-amylase and a maltogenic enzyme to produce a soluble starch substrate that is enzymatically converted to a sugar composition containing higher maltose. The process may use more than one temperature to achieve the same. 1

公开(公告)号：EP2831256A1

公开(公告)日：2015-02-04

申请号：EP13716909.0

申请日：2013-03-26

申请人： Danisco US Inc.

发明人： KOOPS, Bart Christiaan ,  KOOY, Floor K. ,  LEE,Sung Ho ,  SHETTY, Jayarama K. ,  STROHM, Bruce A. ,  VAN TUIJL, Arjen Hendrik

IPC分类号： C12P19/02 ,  C12P19/14 ,  C13K1/06

CPC分类号： C12P19/20 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01003 ,  C13K1/06

摘要： The present teachings provide a method for making a high glucose syrup at a low temperature. In some embodiments, the syrup contains reduced reversion reaction products. The method comprises contacting a starch substrate at a temperature below the starch gelatinization temperature with an enzyme blend comprising a high dose of alpha-amylase and a low dose of glucoamylase. In some embodiments, a blend of two glucoamylases is employed. In some embodiments, a debranching enzyme such as a pullulanase is employed. In some embodiments, the enzymes are staged by adding at different times, or at different temperatures. The present teachings provide for high glucose syrups with fewer reversion products, and allow for higher starch solubilization.

公开(公告)号：EP2831258A2

公开(公告)日：2015-02-04

申请号：EP13712995.3

申请日：2013-03-13

申请人： Danisco US Inc.

发明人： BLAKE, Collette Michelle ,  DUAN, Gang ,  KO, Douglas ,  KOOY, Floor K. ,  LEE, Sung, Ho ,  PEPSIN, Michael J. ,  QIAN, Ying ,  REBOLI, Matthew T. ,  SHARMA, Vivek ,  SHETTY, Jayarama, K. ,  STROHM, Bruce, A. ,  TEUNISSEN, Paula Johanna, Maria ,  XU, Hongxian

IPC分类号： C12P19/12

CPC分类号： C12P19/14 ,  C12P19/02 ,  C12P19/12 ,  C12P19/20 ,  C12Y301/03 ,  C12Y302/01001 ,  C12Y302/01003

摘要： Provided herein are compositions and methods related to the direct conversion of the starch in a ground or fractionated grain into a fermentable sugar feedstock capable of serving as a carbon source for the industrial production of one or more products by a fermenting organism. Such conversions may be performed at temperatures at or below the initial gelatinization temperature of the starch present in the grain and may utilize one or more isolatable endogenous enzymes present in certain unrefined grains.

摘要翻译： 本文提供的是与将研磨或分馏的谷物中的淀粉直接转化成可发酵糖原料相关的组合物和方法，其可以作为用于通过发酵生物体工业生产一种或多种产品的碳源。 这种转化可以在等于或低于晶粒中存在的淀粉的初始糊化温度的温度下进行，并且可以利用存在于某些未精制谷物中的一种或多种可分离的内源性酶。