摘要:
Embodiments of the present disclosure relate to a process for producing isoprene from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
摘要:
The present invention relates to a process for producing downstream products, such as fermentable sugars (e.g., glucose) and alcohols (e.g., ethanol) from starch-containing material (e.g., grain) without a pH adjustment before or after the starch liquefaction step.
摘要:
Provided are GH10 xylanases or a fragment thereof including modified xlyanase enzymes, all having xylanase activity, wherein the enzyme or fragment thereof provides increased oil recovery from a grain-based material compared with a control or parent GH10 xylanase enzyme, the parent GH10 xylanase having been modified at at least one or more of the following positions 7, 25, 33, 64, 79, 89, 217 and 298, wherein the numbering is based on the amino acid numbering of FveXyn4 (SEQ ID No. 1). Methods of using the GH10 xylanases, for example, for improved oil recovery are also provided.
摘要翻译:提供的GH10木聚糖酶或其片段包括全部具有木聚糖酶活性的改性的xlyanase酶,其中与对照或亲本GH10木聚糖酶相比,其酶或片段提供了从谷物基材料提高的油回收率,母本GH10木聚糖酶已被 在以下至少一个或多个位置7,25,33,64,79,89,217和298处修饰,其中编号基于FveXyn4(SEQ ID No.1)的氨基酸编号。 还提供了使用GH10木聚糖酶的方法,例如用于改进的油回收。
摘要:
Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
摘要:
The present disclosure relates to a Bacillus subtilis alpha-amylase (AmyE) or its variant thereof. AmyE or its variants thereof may be used to eliminate or reduce the iodine-positive starch presented in saccharide liquor. Also disclosed are a composition comprising an AmyE or variant thereof and a method utilizing an AmyE or variant thereof to eliminate or reduce the iodine-positive starch.
摘要:
The present disclosure relates to an enzyme blend comprising a low pH, thermostable alpha-amylase and a Bacillus licheniformis alpha-amylase. The blend can include at least about 1.0 Liquefon Unit (LU) of the B. licheniformis alpha-amylase for every 5.0 Modified Wohlgemuth Unit (MWU) of the low pH, thermostable alpha-amylase. The enzyme blend described is suitable for starch liquefaction and saccharification, ethanol production, and/or sweetener production, among other things. Also provided herein is a method of processing a starch by liquefying the starch with the low pH, thermostable alpha-amylase and the Bacillus licheniformis alpha-amylase, simultaneously or sequentially.
摘要:
Variants of B. licheniformis alpha-amylase exhibit improved enzymatic performance, including increased themostability and reduced calcium dependence. Compositions comprising the variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal. The nucleic acids encoding the variants are also disclosed.
摘要:
Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
摘要:
Methods are provided for saccharifying a starch substrate, comprising contacting the starch substrate with a glucoamylase consisting or comprising of the amino acid sequence of Fusarium verticillioides glucoamylase (Fv-GA) and further contacting the starch substrate with at least one additional glucoamylase. Additional methods are provided for saccharifying and fermenting a starch substrate to produce an end product, a biochemical end product and a fermentable beverage using a combination of Fv-GA and at least one additional glucoamylase.