公开(公告)号：EP3353292A1

公开(公告)日：2018-08-01

申请号：EP16848172.9

申请日：2016-09-26

申请人： Novozymes A/S

发明人： GUO, Xinyan ,  KREEL, Nathaniel Edward ,  JUMP, Joseph ,  HOOSS, Melissa C. ,  ROBERTS, Madison ,  VIDAL, Bernardo, Jr. ,  JENSEN, Kenneth ,  FRISNER, Henrik ,  HOFF, Tine ,  LIU, Ye ,  TANG, Lan

IPC分类号： C12N9/54 ,  C12P1/00 ,  C12P7/06 ,  C12N9/30 ,  C12N9/34 ,  C12N9/40 ,  C12N15/63 ,  C12N9/48 ,  C12N9/58 ,  C12N9/50

CPC分类号： C12P7/06 ,  C12N9/2402 ,  C12N9/48 ,  C12N9/50 ,  C12N9/54 ,  C12N9/58 ,  C12N15/63 ,  C12P2203/00 ,  C12Y302/01001 ,  C12Y304/14 ,  C12Y304/21 ,  C13K1/06 ,  Y02E50/17

摘要： S53 proteases and the use of S53 protease in processes for converting starch to ethanol are provided.

公开(公告)号：EP2943594A1

公开(公告)日：2015-11-18

申请号：EP14701857.6

申请日：2014-01-08

申请人： Wisconsin Alumni Research Foundation

发明人： DUMESIC, James A. ,  LUTERBACHER, Jeremy S.

IPC分类号： C13K1/02 ,  C13K13/00 ,  C13K1/06 ,  C07H3/06

CPC分类号： C13K1/02 ,  C07D307/46 ,  C07D307/50 ,  C07H3/06 ,  C13K1/00 ,  C13K1/06 ,  C13K13/002

摘要： A process to produce an aqueous solution of carbohydrates that contains C6-sugar-containing oligomers, C6 sugar monomers, C5-sugar-containing oligomers, C5 sugar monomers, or any combination thereof is presented. The process includes the steps of reacting biomass or a biomass-derived reactant with a solvent system including a lactone and water, and an acid catalyst. The reaction yields a product mixture containing water-soluble C6-sugar-containing oligomers, C6-sugar monomers, C5-sugar-containing oligomers, C5-sugar monomers, or any combination thereof. A solute is added to the product mixture to cause partitioning of the product mixture into an aqueous layer containing the carbohydrates and a substantially immiscible organic layer containing the lactone.

摘要翻译： 以产生碳水化合物wässrige溶液的方法，做了包含C6含糖低聚物，C6糖单体，含有C5-糖的低聚物，C5糖单体，或它们的任何组合被呈现。 的方法包括使生物质或用溶剂系统包含内酯和水，以及酸催化剂生物质衍生反应物的步骤。 反应产生的产物混合物含有水溶性C6含糖低聚物，C6-糖单体，含有C5-糖低聚物，C5-糖单体，或它们的任何组合。 溶质加入到产物混合物以使所述产物混合物分配进入在wässrige层含有碳水化合物和含内酯的基本上不混溶的有机层。

公开(公告)号：EP2832861A4

公开(公告)日：2015-11-18

申请号：EP13770343

申请日：2013-04-01

申请人： SHANDONG HONGHE SUNKEEN BIOTECHNOLOGY CO LTD

发明人： WU YUNSHAN ,  YAN GONGHONG ,  YI YONG ,  KOU ZHENG EN ,  WU ZHEN

IPC分类号： C12P19/20 ,  C12P19/02 ,  C12P19/14

CPC分类号： C12P19/02 ,  A23L29/30 ,  A23L29/35 ,  C12P19/14 ,  C13K1/06 ,  Y02E50/16

摘要： The present invention relates to the field of biology, in particular to a sugar preparation method by using biomass sweet potato dregs for microbial fermentation as a sugar source. The liquid sugar product prepared with the method of the present invention essentially comprises the ingredient of glucose. The method of the present invention is of simple process, high specificity, good product quality and high yield, and solves the serious environmental pollution problem of the sweet potato dregs, thus having a good industrial application prospect.

摘要翻译： 本发明涉及生物学领域，特别涉及通过使用生物量甘薯渣作为糖源的微生物发酵制糖方法。 用本发明方法制备的液体糖产品基本上包含葡萄糖成分。 本发明方法工艺简单，特异性高，产品质量好，产量高，解决了红薯渣严重的环境污染问题，具有良好的工业应用前景。

公开(公告)号：EP2931912A1

公开(公告)日：2015-10-21

申请号：EP13815246.7

申请日：2013-12-05

申请人： Danisco US Inc.

发明人： HUA, Ling ,  SCHEFFERS, Martijn ,  VAN BRUSSEL, Marco ,  VROEMEN, Casper ,  ZHANG, Bo ,  ZHANG, Zhenghong

IPC分类号： C12P19/14 ,  C12N9/30 ,  A21D8/04 ,  A23L1/10 ,  C11D3/386 ,  D06M16/00

CPC分类号： C12P19/02 ,  A21D8/042 ,  A23L2/382 ,  C11D3/38636 ,  C12C5/004 ,  C12C7/04 ,  C12C7/16 ,  C12C11/003 ,  C12C12/04 ,  C12G3/02 ,  C12N9/242 ,  C12P7/14 ,  C12P19/14 ,  C12Y302/01001 ,  C13K1/06 ,  D06L1/06

摘要： A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmy1). AfAmy1 has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

公开(公告)号：EP2931910A1

公开(公告)日：2015-10-21

申请号：EP13808307.6

申请日：2013-11-21

申请人： Danisco US Inc.

发明人： GE, Jing ,  HUA, Ling ,  LEE, Sung Ho ,  SHETTY, Jayarama K. ,  STROHM, Bruce A. ,  TANG, Zhongmei ,  WARD, Donald E.

IPC分类号： C12P19/14 ,  C12P19/20 ,  C13K1/06 ,  C12C5/02

CPC分类号： C12P19/16 ,  C12C5/004 ,  C12C12/00 ,  C12P19/02 ,  C12P19/14 ,  C12P19/20 ,  C13K1/06

摘要： Fungal glucoamylases from
Aspergillus fumigatus that are expressed in
Trichoderma reesei host cells (AfGATR) are provided. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a simultaneous liquefaction and saccharification process in the presence of alpha amylase, such as A
spergillus kawachii alpha-amylase (AkAA). This greatly reduces the combined run time of liquefaction and saccharification reaction, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase.

公开(公告)号：EP2831256A1

公开(公告)日：2015-02-04

申请号：EP13716909.0

申请日：2013-03-26

申请人： Danisco US Inc.

发明人： KOOPS, Bart Christiaan ,  KOOY, Floor K. ,  LEE,Sung Ho ,  SHETTY, Jayarama K. ,  STROHM, Bruce A. ,  VAN TUIJL, Arjen Hendrik

IPC分类号： C12P19/02 ,  C12P19/14 ,  C13K1/06

CPC分类号： C12P19/20 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01003 ,  C13K1/06

摘要： The present teachings provide a method for making a high glucose syrup at a low temperature. In some embodiments, the syrup contains reduced reversion reaction products. The method comprises contacting a starch substrate at a temperature below the starch gelatinization temperature with an enzyme blend comprising a high dose of alpha-amylase and a low dose of glucoamylase. In some embodiments, a blend of two glucoamylases is employed. In some embodiments, a debranching enzyme such as a pullulanase is employed. In some embodiments, the enzymes are staged by adding at different times, or at different temperatures. The present teachings provide for high glucose syrups with fewer reversion products, and allow for higher starch solubilization.

公开(公告)号：EP2733209A2

公开(公告)日：2014-05-21

申请号：EP13190341.1

申请日：2008-02-06

申请人： Danisco US, Inc., Genencor Division

发明人： Cervin, Marguerite A. ,  Kensch, Oliver ,  Kettling, Ulrich ,  Kim, Steve ,  Leuthner, Birgitta ,  Miasnikov, Andrei ,  Pellengahr, Klaus ,  Ward, Michael

IPC分类号： C12N15/55 ,  C12N9/16 ,  A23K1/165

CPC分类号： C12N9/16 ,  A23K20/189 ,  A23K50/10 ,  A23K50/30 ,  A23K50/75 ,  A23K50/80 ,  C12Y301/03008 ,  C12Y301/03026 ,  C13K1/06 ,  Y02A40/818 ,  Y02E50/17

摘要： The present invention relates to variant phytase enzymes having altered properties.

摘要翻译： 本发明涉及具有改变性质的变体植酸酶。

公开(公告)号：EP2718449A1

公开(公告)日：2014-04-16

申请号：EP12727087.4

申请日：2012-06-07

申请人： Xyleco, Inc.

发明人： MEDOFF, Marshall ,  BAE, Seul-a ,  VALDEZ, Randy ,  MASTERMAN, Thomas Craig

IPC分类号： C12P7/10 ,  C13K1/02 ,  C08H8/00 ,  B01F7/00 ,  C08J3/00

CPC分类号： C13K1/02 ,  B01F3/1271 ,  B01F5/0206 ,  B01F5/0212 ,  B01F5/0275 ,  B01F5/106 ,  B01F7/00241 ,  B01F7/00358 ,  B01F7/00383 ,  B01F7/0075 ,  B01F7/00808 ,  B01F7/1635 ,  B01F13/10 ,  B01F15/00246 ,  B01F15/00538 ,  B01F15/0283 ,  B02C18/062 ,  C08H8/00 ,  C08J3/28 ,  C08J2301/02 ,  C10G1/00 ,  C10G32/00 ,  C10G2300/1014 ,  C12M45/09 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  C13K1/06 ,  C13K13/002 ,  Y02E50/13 ,  Y02E50/16 ,  Y02P30/20

摘要： Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce a product such as a biofuel.

摘要翻译： 加工生物质原料（例如，植物生物质，动物生物质和城市废物生物质）以产生有用的产物，例如燃料。 例如，描述了可以将原料转化为糖溶液的系统，然后将其进行发酵以产生诸如生物燃料的产物。

公开(公告)号：EP2340313A2

公开(公告)日：2011-07-06

申请号：EP09796432.4

申请日：2009-10-28

申请人： Pursuit Dynamics PLC.

发明人： FENTON, Marcus Brian, Mayhall ,  THORUP, Jens, Havn

IPC分类号： C12P19/14 ,  C12P7/10 ,  C12M1/40 ,  C13K1/06 ,  B01F5/04 ,  B01F3/12

CPC分类号： C12P7/10 ,  A23V2002/00 ,  B01F3/1214 ,  B01F3/1271 ,  B01F5/0413 ,  B01F5/0426 ,  B01F5/043 ,  C08H8/00 ,  C12M21/12 ,  C12M29/14 ,  C12M43/02 ,  C12M45/02 ,  C12M45/09 ,  C12M45/20 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C13K1/02 ,  C13K1/06 ,  D21C1/02 ,  D21C5/00 ,  D21C11/0007 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P20/136 ,  A23V2250/082 ,  A23V2250/5108 ,  A23V2250/5118

摘要： The present invention provides processes and systems for treating biomass and, e.g., making biofuels, such as bioethanol, from the biomass. More particularly, one process according to the present invention includes (a) inducing at least a first portion of a composition containing biomass and a working fluid to flow into a passage of a fluid processing apparatus, (b) injecting a high velocity transport fluid into the composition through a nozzle communicating with the passage of the fluid processing apparatus, whereby the transport fluid applies a shear force to the composition such that the working fluid is atomised and a vapour and droplet flow regime is formed downstream of the nozzle, (c) condensing the vapour and droplet flow regime, (d) transferring the composition to a first holding vessel, and (e) holding the composition in the first holding vessel at a first predetermined temperature for a first predetermined period of time, wherein a liquefaction enzyme is added to the composition prior to or during the process. Thereafter, the composition may be further processed to form a biofuel, such as, e.g., bioethanol.

摘要翻译： 本发明提供了用于处理生物质并且例如从生物质制备生物燃料例如生物乙醇的方法和系统。 更具体地说，根据本发明的一种方法包括：（a）诱导含有生物质和工作流体的组合物的至少第一部分流入流体处理设备的通道中，（b）将高速输送流体注入 所述组合物通过与所述流体处理设备的通道连通的喷嘴，由此所述输送流体向所述组合物施加剪切力，使得所述工作流体被雾化并且在所述喷嘴的下游形成蒸汽和液滴流动状态，（c） （d）将该组合物转移到第一保持容器中，和（e）在第一预定温度下将组合物保持在第一保持容器中第一预定时间段，其中液化酶是 在该过程之前或过程中添加到组合物中。 此后，组合物可以被进一步处理以形成生物燃料，例如生物乙醇。

公开(公告)号：EP2329033A1

公开(公告)日：2011-06-08

申请号：EP09810526.5

申请日：2009-08-26

申请人： Edeniq, Inc.

发明人： GALVEZ III, Adriano ,  RICHARDS, Glenn

IPC分类号： C12P19/00

CPC分类号： C12P19/14 ,  C08H8/00 ,  C12P7/10 ,  C12P7/14 ,  C12P19/02 ,  C12P2201/00 ,  C13K1/02 ,  C13K1/06 ,  Y02E50/16 ,  Y02E50/17 ,  Y02T50/678

摘要： This disclosure provides for materials and methods for converting biomass to biofuels. The materials include a colloid mill with or without cellulase enzymes, and the methods include the use of a colloid mill and optionally cellulose enzymes to pretreat biomass for use in a biomass to biofuel production process.