公开(公告)号：EP2904105A1

公开(公告)日：2015-08-12

申请号：EP13812353.4

申请日：2013-12-06

申请人： Danisco US Inc.

发明人： GE, Jing ,  HUA, Ling ,  SCHEFFERS, Martijn ,  VAN BRUSSEL, Marco ,  VROEMEN, Casper ,  ZHANG, Bo

IPC分类号： C12P19/14 ,  C12N9/24 ,  A21D8/04 ,  A23L1/10 ,  C11D3/386 ,  C12C11/00 ,  D06M16/00

CPC分类号： C12P19/14 ,  A21D8/042 ,  A23L2/382 ,  A23L7/107 ,  A23L29/35 ,  C11D3/386 ,  C12C5/004 ,  C12C7/04 ,  C12C7/16 ,  C12C11/003 ,  C12C12/04 ,  C12N9/242 ,  C12P7/06 ,  C12P7/14 ,  C12P19/02 ,  C12P2203/00 ,  C13K1/06 ,  C13K11/00 ,  D06L1/06 ,  Y02E50/17

摘要： A fungal alpha-amylase is provided from Aspergillus terreus (AtAmy1). AtAmy1 has an optimal pH of 4.5 and is operable at 30 75 degrees C, allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AtAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1 + DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

公开(公告)号：EP2931912A1

公开(公告)日：2015-10-21

申请号：EP13815246.7

申请日：2013-12-05

申请人： Danisco US Inc.

发明人： HUA, Ling ,  SCHEFFERS, Martijn ,  VAN BRUSSEL, Marco ,  VROEMEN, Casper ,  ZHANG, Bo ,  ZHANG, Zhenghong

IPC分类号： C12P19/14 ,  C12N9/30 ,  A21D8/04 ,  A23L1/10 ,  C11D3/386 ,  D06M16/00

CPC分类号： C12P19/02 ,  A21D8/042 ,  A23L2/382 ,  C11D3/38636 ,  C12C5/004 ,  C12C7/04 ,  C12C7/16 ,  C12C11/003 ,  C12C12/04 ,  C12G3/02 ,  C12N9/242 ,  C12P7/14 ,  C12P19/14 ,  C12Y302/01001 ,  C13K1/06 ,  D06L1/06

摘要： A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmy1). AfAmy1 has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

公开(公告)号：EP2935606A1

公开(公告)日：2015-10-28

申请号：EP13815269.9

申请日：2013-12-06

申请人： Danisco US Inc.

发明人： GE, Jing ,  HUA, Ling ,  SCHEFFERS, Martijn ,  VAN BRUSSEL, Marco ,  VROEMEN, Casper ,  ZHANG, Bo

IPC分类号： C12P19/14 ,  C12N9/30 ,  A21D8/04 ,  A23L1/10 ,  C11D3/386 ,  C12C11/00 ,  D06M16/00

CPC分类号： C12P19/14 ,  A21D8/042 ,  A23L2/382 ,  A23L7/107 ,  A23L29/35 ,  C11D3/38618 ,  C11D3/38681 ,  C12C5/004 ,  C12N9/242 ,  C12P7/14 ,  C12P19/02 ,  D06L1/14 ,  D06M16/003

摘要： A fungal alpha-amylase is provided from
Aspergillus terreus (AtAmy1). AtAmy1 has an optimal pH of 4.5 and is operable at 30 75 degrees C, allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AtAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1 + DP2) compared to the products of saccharification catalyzed by an alpha amylase from
Aspergillus kawachii . This facilitates the utilization of the ligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

公开(公告)号：EP2931911A1

公开(公告)日：2015-10-21

申请号：EP13811709.8

申请日：2013-12-05

申请人： Danisco US Inc.

发明人： HUA, Ling ,  SCHEFFERS, Martijn ,  VAN BRUSSEL, Marco ,  VROEMEN, Casper ,  ZHANG, Bo ,  ZHANG, Zhenghong

IPC分类号： C12P19/14 ,  C12N9/28 ,  C12P7/06 ,  C12C11/00 ,  A23L1/105 ,  A21D8/04 ,  C11D3/386 ,  D06M16/00

CPC分类号： C12P19/14 ,  A21D8/042 ,  A23L7/107 ,  A23V2002/00 ,  C11D3/386 ,  C12C5/004 ,  C12C11/003 ,  C12N9/242 ,  C12N9/246 ,  C12P7/06 ,  C12P19/02 ,  C12Y302/01001 ,  C12Y302/01068 ,  D06L1/06 ,  Y02E50/17

摘要： A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

摘要翻译： 从烟曲霉（AfAmyl）提供真菌α-淀粉酶。 AfAmyl具有3.5的最佳pH，并且在30-75摄氏度下可操作，使得酶可以在糖化反应中与葡糖淀粉酶和异淀粉酶组合使用。 这消除了作为间歇方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AfAmyl还催化淀粉底物对显着富含DP2和（DPI + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中通过发酵生物体利用寡糖组合物。