公开(公告)号：EP1149163A2

公开(公告)日：2001-10-31

申请号：EP00901252.7

申请日：2000-01-31

申请人： Delta Biotechnology Limited

发明人： VAN URK, Hendrik,Delta Biotechnology Limited ,  MEAD, David John,Delta Biotechnology Limited ,  MORTON, Philip Harvey,Delta Biotechnology Limited ,  CARTWRIGHT, Andrew John,Delta Biotechnology Ltd ,  CAMERON, Jason,Delta Biotechnology Limited ,  BALLANCE, David James,Aventis Behring ,  GRANDGEORGE, Michel G. J.,Aventis Behring GmbH ,  BEREZENKO, Stephen,Delta Biotechnology Limited ,  WOODROW, John Rodney,Delta Biotechnology Limited ,  SLEEP, Darrell,Delta Biotechnology Limited ,  VERON, Jean-Luc Bernard,Delta Biotechnology Ltd

IPC分类号： C12N15/14 ,  C12N15/81 ,  C07K1/18 ,  C07K1/22 ,  C12P21/02

CPC分类号： B01D15/3804 ,  B01D15/362 ,  B01D15/363 ,  C07K14/76 ,  C07K14/765

摘要： A process is provided for the preparation of a highly pure albumin solution the process comprising subjecting albumin (preferably expressed and secreted by transformed yeast) to a series of chromatographic steps. Preferably, the process comprises the steps of positive mode cation exchange chromatography, positive mode anion exchange chromatography, positive mode affinity chromatography, negative mode affinity chromatography (preferably using immobilised aminophenylboronic acid), negative mode cation exchange chromatography, and negative or positive mode anion exchange chromatography. A process for reducing the level of nickel in an albumin solution is also disclosed, as is a recombinant albumin coding sequence comprising two or more in-frame translation stop codons. Also disclosed is a process for producing recombinant albumin, the process comprising culturing a fungal cell expressing a recombinant albumin coding sequence, wherein the cell has a reduced capacity of mannosylation of the recombinantly-expressed albumin.