公开(公告)号：EP1499709A2

公开(公告)日：2005-01-26

申请号：EP03721832.8

申请日：2003-04-22

申请人： E. I. du Pont de Nemours and Company ,  GENENCOR INTERNATIONAL, INC.

发明人： PAYNE, Mark, S. ,  PICATAGGIO, Stephen, K. ,  TRIMBUR, Donald, Eugene ,  HSU, Amy, Kuang-Hua ,  VALLE, Fernando ,  NAIR, Ramesh ,  SOUCAILLE, Philippe

IPC分类号： C12N1/21 ,  C12N9/90 ,  C12N15/61 ,  C12N15/63 ,  C07H21/04

CPC分类号： C07K14/36 ,  C12N9/90 ,  C12N15/70 ,  C12N15/76 ,  C12P7/18

摘要： This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.

公开(公告)号：EP1620543B1

公开(公告)日：2016-11-23

申请号：EP04751769.3

申请日：2004-05-07

申请人： E. I. du Pont de Nemours and Company

发明人： PICATAGGIO, Stephen, K. ,  YADAV, Narendra, S. ,  ZHU, Quinn, Qun

IPC分类号： C12P7/64 ,  C12N9/02

CPC分类号： C12N15/815 ,  C12P7/6427 ,  C12P7/6472