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    METHODS AND COMPOSITIONS FOR PRODUCING MALE STERILE PLANTS
    2.
    发明公开
    METHODS AND COMPOSITIONS FOR PRODUCING MALE STERILE PLANTS 审中-公开
    VERFAHREN UND ZUSAMMENSETZUNGEN ZUR HERSTELLUNG STERILERMÄNNLICHERPFLANZEN

    公开(公告)号：EP2723874A2

    公开(公告)日：2014-04-30

    申请号：EP12823277.4

    申请日：2012-06-19

    申请人： Pioneer Hi-Bred International, Inc. ,  E. I. Du Pont de Nemours and Company

    发明人： BIDNEY, Dennis L. ,  CIGAN, Mark ,  FALCO, Carl ,  GAO, Huirong ,  JANTZ, Derek ,  LASSNER, Mike ,  LOWE, Keith ,  LYZNIK, Leszek, Aleksander ,  SMITH, James, Jefferson

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8289 ,  C12N5/14 ,  C12N9/22 ,  C12N15/8213 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Y301/00

    摘要： Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a eel! comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand- break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.

    摘要翻译： 公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。 所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。 还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。 包含其中具有至少一种靶向修饰的雄性生殖力基因的核酸分子，编码作为工程改造的双链断裂诱导剂和表达盒的核酸内切酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物 进一步公开。

    TRANSGENIC CEREAL PLANTS
    3.
    发明公开
    TRANSGENIC CEREAL PLANTS 失效
    转基因谷物

    公开(公告)号：EP0772687A2

    公开(公告)日：1997-05-14

    申请号：EP95933706.0

    申请日：1995-07-26

    申请人： PIONEER HI-BRED INTERNATIONAL, INC.

    发明人： BOWEN, Benjamin, A. ,  LOWE, Keith ,  ROSS, Margot, C. ,  SANDAHL, Gary, A. ,  TOMES, Dwight, T. ,  SONGSTAD, David, D. ,  GORDON-KAMM, William, J.

    IPC分类号： A01H5 ,  C12N15

    CPC分类号： C12N15/8207

    摘要： To obtain a transgenic cereal plant which is stably transformed, an exposed cereal meristem is subjected to biolistic bombardment in order to target non-differentiated meristem cells for transformation. Immature embryos at the early proembryo, mid proembryo, late proembryo, transitional or early coleoptilar stage are harvested for biolistic bombardment. The meristem tissue or cells fated to contribute to the meristem then are manipulated in order to enlarge transgenic sectors, either through selection and/or through effecting a proliferation from the tissue of shoots or multiple meristems per se. The shoot population thus obtained then is screened, by means of a nonlethal enrichment assay, to identify either chimeric sectors that will contribute to germline transmission, or non-sectored, L2 periclinal chimeras that will by definition transmit to progeny. Increased time in culture, under selection, enhances the prospects for sectoral-to-periclinal conversions, and also selects for L1-to-L2 conversions which, through a shift in position, ultimately contribute to the germline. Transgenic sectors also are stabilized during the step of tillering.