利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

461 条记录 (耗时 0.026 秒)

    COMPOSITIONS AND METHODS FOR MODIFYING GENOMES
    1.
    发明公开
    COMPOSITIONS AND METHODS FOR MODIFYING GENOMES 审中-公开

    公开(公告)号：EP4407034A2

    公开(公告)日：2024-07-31

    申请号：EP24171775.0

    申请日：2018-08-08

    申请人： RiceTec, Inc.

    发明人： BEGEMANN, Matthew ,  GRAY, Benjamin N.

    IPC分类号： C12N15/82

    CPC分类号： C12N9/22 ,  C12N15/102 ,  C12N15/8213 ,  C12N15/825 ,  C12N15/8269

    摘要： Compositions and methods for modifying genomic DNA sequences are provided. The methods produce double-stranded breaks (DSBs) at pre-determined target sites in a genomic DNA sequence, resulting in mutation, insertion, and/or deletion of DNA sequences at the target site(s) in a genome. Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cms1 protein operably linked to a promoter that is operable in the cells of interest. The DNA constructs can be used to direct the modification of genomic DNA at pre-determined genomic loci. Methods to use these DNA constructs to modify genomic DNA sequences are described herein. Additionally, compositions and methods for modulating the expression of genes are provided. Compositions comprise DNA constructs comprising a promoter that is operable in the cells of interest operably linked to nucleotide sequences that encode a mutated Cms1 protein with an abolished ability to produce DSBs, optionally linked to a domain that regulates transcriptional activity. The methods can be used to up- or down-regulate the expression of genes at predetermined genomic loci.

    ALGAL MUTANTS HAVING A LOCKED-IN HIGH LIGHT ACCLIMATED PHENOTYPE
    5.
    发明公开
    ALGAL MUTANTS HAVING A LOCKED-IN HIGH LIGHT ACCLIMATED PHENOTYPE 有权
    阿尔茨海默病麻醉药自身免疫缺陷病毒

    公开(公告)号：EP2929029A4

    公开(公告)日：2016-11-16

    申请号：EP13860017

    申请日：2013-12-06

    申请人： SYNTHETIC GENOMICS INC

    发明人： BAILEY SHAUN ,  MCCARREN JAY ,  LIEBERMAN SOYAN LEUNG ,  MEUSER JONATHAN E ,  ROMANO ANNA E ,  YEE DANIEL ,  SORIAGA LEAH ,  BROWN ROBERT C ,  WEISSMAN JOSEPH C ,  PRINCE ROGER C ,  NIELSEN ROBERT D ,  SCHWARTZ ARIEL S

    IPC分类号： C12N1/12 ,  C07K14/405 ,  C07K14/415 ,  C12N1/36 ,  C12N15/01 ,  C12N15/11 ,  C12N15/82 ,  C12N15/87

    CPC分类号： C12N15/825 ,  C07K14/405 ,  C07K14/415 ,  C12N1/12 ,  C12N1/36 ,  C12N15/01 ,  C12N15/8269

    摘要： Mutant photosynthetic microorganisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutants have a locked in high light-acclimated phenotype, in which many of the photosynthetic parameters characteristic of high light acclimated wild type cells are found in the LIHLA mutants when acclimated to low light, such as reduced chlorophyll, reduced NPQ, higher qP, higher Ek, higher Pmax per unit chlorophyll with little to no reduction in Pmax per cell, and higher rates of electron transport through photosystem II over a wide range of light intensities. Provided herein are constructs for attenuating or disrupting genes are provided for generating mutants having the LIHLA phenotype. Also provided are methods of culturing LIHLA mutants for the production of biomass or other products.

    摘要翻译： 提供了具有降低的叶绿素和增加的光合效率的突变的光合微生物。 突变体锁定在高光适应表型中，其中许多光照适应度高的野生型细胞的光合参数在适应低光条件下发现在LIHLA突变体中，如叶绿素降低，NPQ降低，qP升高， 较高的Ek，每单位叶绿素具有更高的Pmax，每个细胞的Pmax几乎不降低，并且在宽范围的光强度下具有较高的通过光系统II的电子传输速率。 本文提供了用于减弱或破坏基因的构建物，用于产生具有LIHLA表型的突变体。 还提供培养用于生产生物质或其他产品的LIHLA突变体的方法。