公开(公告)号：EP1297163B1

公开(公告)日：2011-04-27

申请号：EP01952192.1

申请日：2001-06-22

申请人： E.I. DU PONT DE NEMOURS AND COMPANY ,  PIONEER HI-BRED INTERNATIONAL, INC.

发明人： GLASSMAN, Kimberly, F. ,  GORDON-KAMM, William, J. ,  KINNEY, Anthony, J. ,  LOWE, Keith ,  STECCA, Kevin, L. ,  NICHOLS, Scott, E.

IPC分类号： C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8216 ,  C12N15/8218 ,  C12N15/8245 ,  C12N15/8246 ,  C12N15/8247 ,  C12N15/825 ,  C12N15/8251

摘要： Recombinant constructs useful for reducing the expression of endogenous mRNA and any substantially similar endogenous mRNA are disclosed. In particular, a recombinant construct comprising, inter alia, a suitable nucleic acid sequence and its reverse complement can be used to alter the expression of any homologous, endogenous RNA (i.e., the target RNA) which is in proximity to this suitable nucleic acid sequence.

公开(公告)号：EP1907549A1

公开(公告)日：2008-04-09

申请号：EP06773419.4

申请日：2006-06-16

申请人： Pioneer-Hi-Bred International, Inc. ,  E.I. du Pont de Nemours and Company ,  Plant Bioscience Limited Norwich (GB)

发明人： BAULCOMBE, David ,  KREBBERS, Enno ,  HITZ, William, D. ,  GLASSMAN, Kimberly, F. ,  AUKERMAN, Milo, J. ,  WILLIAMS, Robert ,  YOO, Byung-chun

IPC分类号： C12N15/82 ,  C12N5/04 ,  A01H5/00 ,  A01H5/10 ,  A01H3/00

CPC分类号： C12N15/8218 ,  C07H21/02 ,  C07H21/04 ,  C12N2310/14

摘要： Methods and compositions are provided for reducing the level of expression of a target polynucleotide in an organism. The methods and compositions selectively silence the target polynucleotide through the expression of a chimeric polynucleotide comprising the target for a sRNA (the trigger sequence) operably linked to a sequence corresponding to all or part of the gene or genes to be silenced. In this manner, the final target of silencing is an endogenous gene in the organism in which the chimeric polynucleotide is expressed. In a further embodiment, the miRNA target is that of a heterologous miRNA or siRNA, the latter of which is coexpressed in the cells at the appropriate developmental stage to provide silencing of the final target when and where desired. In a further embodiment, the final target may be a gene in a second organism, such as a plant pest, that feeds upon the organism containing the chimeric gene or genes. Compositions further comprise vectors, seeds, grain, cells, and organisms, including plants and plant cells, comprising the chimeric polynucleotide of the invention.

公开(公告)号：EP1297163A2

公开(公告)日：2003-04-02

申请号：EP01952192.1

申请日：2001-06-22

申请人： E. I. du Pont de Nemours and Company ,  PIONEER HI-BRED INTERNATIONAL, INC.

发明人： GLASSMAN, Kimberly, F. ,  GORDON-KAMM, William, J. ,  KINNEY, Anthony, J. ,  LOWE, Keith ,  STECCA, Kevin, L. ,  NICHOLS, Scott, E.

IPC分类号： C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8216 ,  C12N15/8218 ,  C12N15/8245 ,  C12N15/8246 ,  C12N15/8247 ,  C12N15/825 ,  C12N15/8251

摘要： Recombinant constructs useful for reducing the expression of endogenous mRNA and any substantially similar endogenous mRNA are disclosed. In particular, a recombinant construct comprising, inter alia, a suitable nucleic acid sequence and its reverse complement can be used to alter the expression of any homologous, endogenous RNA (i.e., the target RNA) which is in proximity to this suitable nucleic acid sequence.