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1.发明公开YARROWIA PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE PROMOTER REGIONS FOR GENE EXPRESSION IN YEAST 审中-公开过氧化物酶体2.4二烯酰COA REDUKTASEPROMOTERREGIONEN OFF耶氏在酵母基因表达
公开(公告)号：EP2702148A1
公开(公告)日：2014-03-05
申请号：EP12712873.4
申请日：2012-04-02
发明人： ZHU, Quinn Qun , HONG, Seung-Pyo
CPC分类号： C12N15/815 , C12N9/001 , C12P7/6427 , C12P21/02 , C12Y103/01034
摘要： Promoter regions associated with the
Yarrowia lipolytica peroxisomal 2,4-dienoyl-CoA reductase (
SPS19 ) gene are disclosed and have been found to be particularly effective for the expression of heterologous genes in yeast. These promoter regions will be useful for driving high-level expression of genes involved in the production of omega-3 and omega-6 fatty acids.-
2.发明公开MUTANT HPGG MOTIF AND HDASH MOTIF DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS 审中-公开MUTANT HPGG-动机和HDASH MOTIF DELTA 5-去饱和酶和它们在多不饱和脂肪酸的生产
公开(公告)号：EP2609202A1
公开(公告)日：2013-07-03
申请号：EP11751773.0
申请日：2011-08-26
发明人： BOSTICK, Michael, W. , DAUBERT, Melissa, D. , HE, Hongxian , HONG, Seung-Pyo , POLLAK, Dana, M., Walters , SHARPE, Pamela, L. , WANG, Yehong, Jamie , XUE, Zhixiong , YADAV, Narendra, S. , ZHANG, Hongxiang , ZHU, Qun
CPC分类号： C12N9/001 , C12P7/6472 , C12Y103/01035
摘要： Mutant delta-5 desaturases, having the ability to convert dihomo-gamma-linolenic acid [DGLA; 20:3 omega-6] to arachidonic acid [ARA; 20:4 omega-6] and/or eicosatetraenoic acid [ETA; 20:4 omega-3] to eicosapentaenoic acid [EPA; 20:5 omega-3] and possessing at least one mutation within the HPGG (SEQ ID NO:7) motif of the cytochome b5-like domain and at least one mutation within the HDASH (SEQ ID NO:8) motif are disclosed. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturases, along with a method of making long chain polyunsaturated fatty acids ["PUFAs"], are also disclosed.
摘要翻译： 突变型Δ-5去饱和酶，具有将二高-γ-亚麻酸[DGLA的能力; 20：3ω-6]成花生四烯酸[ARA; 20：4ω-6]和/或二十碳四烯酸[ETA; 20：4ω-3]至二十碳五烯酸[EPA; 20：5ω-3]和波塞唱HPGG（SEQ ID NO：7）内的至少一个突变的细胞色素B5样结构域的基序和HDASH内的至少一个突变（SEQ ID NO：8）基序盘游离缺失。 分离的核酸片段和重组构建体，其包含编码Δ-5去饱和酶检查片段，与制备长链多不饱和脂肪酸[“PUFA的”]的方法一起是游离缺失盘。
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3.发明公开INCREASED OIL CONTENT BY INCREASING YAP1 TRANSCRIPTION FACTOR ACTIVITY IN OLEAGINOUS YEASTS 审中-公开增加的油含量通过增加YAP1转录因子活性油性酵母
公开(公告)号：EP2658985A1
公开(公告)日：2013-11-06
申请号：EP11853498.1
申请日：2011-12-29
发明人： HONG, Seung-Pyo , XUE, Zhixiong , ZHU, Quinn, Qun
IPC分类号： C12P7/64
CPC分类号： C07K14/39 , C12P7/6409 , C12P7/6427 , C12P7/6463
摘要： Transgenic oleaginous yeast having increased oil content comprising increased Yap1 transcription factor activity, wherein the increased oil content is compared to the oil content of a non-transgenic oleaginous yeast, are described herein. The increased Yap1 transcription factor activity results from overexpressing a Yap1 transcription factor, by increasing the interaction between the transcription factor and a protein that is capable of activating the transcription factor, or by a combination thereof. Methods of using these yeast strains are also described.
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4.发明公开USE OF SACCHAROMYCES CEREVISIAE SUC2 GENE IN YARROWIA LIPOLYTICA FOR SUCROSE UTILIZATION 审中-公开酿酒酵母SUC2创作者脂耶氏酵母用于使用蔗糖
公开(公告)号：EP2658962A1
公开(公告)日：2013-11-06
申请号：EP11788953.5
申请日：2011-11-15
发明人： HONG, Seung-Pyo , SEIP, John E. , ZHU, Quinn, Qun
CPC分类号： C12N9/2431 , C07K2319/02 , C12N9/60 , C12P7/64 , C12Y302/01026
摘要： Disclosed herein are transformed
Yarrowia lipolytica comprising an exogenous polynucleotide encoding a polypeptide having sucrose invertase activity. Also disclosed are methods of using the transformed
Y. lipolytica.-
5.发明公开
公开(公告)号：EP2609203A1
公开(公告)日：2013-07-03
申请号：EP11752426.4
申请日：2011-08-26
发明人： HONG, Seung-Pyo , SHARPE, Pamela, L. , XUE, Zhixiong , YADAV, Narendra, S. , ZHANG, Hongxiang , ZHU, Qun
CPC分类号： C12N9/1029 , C12N9/0071 , C12N15/815 , C12P7/6472
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica are disclosed herein that are capable of producing microbial oil comprising greater than 25 weight percent of eicosapentaenoic acid [“EPA”], an omega-3 polyunsaturated fatty acid, measured as a weight percent of dry cell weight.
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6.发明公开PENTOSE PHOSPHATE PATHWAY UPREGULATION TO INCREASE PRODUCTION OF NON-NATIVE PRODUCTS OF INTEREST IN TRANSGENIC MICROORGANISMS 审中-公开磷酸戊糖路径UP FOR控制权益之在转基因生物产量增加非本地产品
公开(公告)号：EP2553107A2
公开(公告)日：2013-02-06
申请号：EP11763298.4
申请日：2011-03-29
发明人： HONG, Seung-Pyo , XUE, Zhixiong , ZHU, Quinn, Qun
CPC分类号： C12P7/6427 , C12N9/0006 , C12N9/18 , C12P7/6472 , C12P7/66 , C12P23/00 , C12P33/00
摘要： Coordinately regulated over-expression of the genes encoding glucose 6-phosphate dehydrogenase ["G6PDH"] and 6-phospho-gluconolactonase ["6PGL"] in transgenic strains of the oleaginous yeast,
Yarrowia lipolytica , comprising a functional polyunsaturated fatty acid ["PUFA"] biosynthetic pathway, resulted in increased production of PUFAs and increased total lipid content in the
Yarrowia cells. This is achieved by increased cellular availability of the reduced form of nicotinamide adenine dinucleotide phosphate ["NADPH"], an important reducing equivalent for reductive biosynthetic reactions, within the transgenic microorganism.
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