公开(公告)号：EP1531664A2

公开(公告)日：2005-05-25

申请号：EP03742192.2

申请日：2003-06-25

申请人： E.I. du Pont de Nemours and Company

发明人： ABAD, Andre, R. ,  FLANNAGAN, Ronald, D. ,  HERRMANN, Rafael ,  KAHN, Theodore, W. ,  LU, Albert, Laurence ,  MCCUTCHEN, Billy Fred ,  PRESNAIL, James, K. ,  WONG, James, F., H. ,  YU, Cao-Guo

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/32 ,  C07K14/325 ,  C12Q1/25

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding delta-endotoxins having pesticidal activity against insect pests. The invention further provides mutagenized nucleic acids that have been modified to encode pesticidal polypeptides such as endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins that may be optimized as well as pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

公开(公告)号：EP1716230A2

公开(公告)日：2006-11-02

申请号：EP05805019.6

申请日：2005-02-18

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. du Pont de Nemours and Company

发明人： ABAD, Andre, R. ,  MCCUTCHEN, Billy, F. ,  WONG, James, F. ,  YU, Cao, Guo

IPC分类号： C12N9/20

CPC分类号： C12N15/8286 ,  C12N9/20 ,  Y02A40/162

摘要： Methods of creating and enhancing insect resistance in plants by introducing non-plant lipases into plants are provided. Plants with enhanced insect resistance and seed from plants thereof are provided. DNA sequences encoding insecticidal lipases and insecticidal lipase gene products that are useful in the practice of this invention are also provided. The compositions and methods of the invention may be used in a variety of agricultural systems for controlling pests, including propagating lineages of insect-resistant crops and targeting expression of these insecticidal lipases to plant organs that are particularly susceptible to infestation, such as roots and leaves.

公开(公告)号：EP1356054B1

公开(公告)日：2006-09-06

申请号：EP01988728.0

申请日：2001-10-24

申请人： E.I. DU PONT DE NEMOURS AND COMPANY

发明人： ABAD, Andre, R. ,  DUCK, Nicholas, B. ,  FENG, Xiang ,  FLANNAGAN, Ronald, D. ,  KAHN, Theodore, W. ,  SIMS, Lynne, E.

IPC分类号： C12N15/31 ,  C12N15/63 ,  C12N15/82 ,  C07K14/325 ,  A01H5/00 ,  A01H5/10 ,  A01N63/00 ,  A01N63/02

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding delta-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

摘要翻译： 本发明提供从编码具有杀虫活性的针对鞘翅目害虫的δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段。 本发明还提供已被修饰以编码具有改进的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。 本发明的具体实施方案提供编码杀虫蛋白的分离的核酸，包含这种核酸的表达盒，以及包含本发明核酸的转化植物和种子。 这些组合物可用于防治害虫，特别是植物害虫的方法。

公开(公告)号：EP1356054A2

公开(公告)日：2003-10-29

申请号：EP01988728.0

申请日：2001-10-24

申请人： E.I. DU PONT DE NEMOURS AND COMPANY

发明人： ABAD, Andre, R. ,  DUCK, Nicholas, B. ,  FENG, Xiang ,  FLANNAGAN, Ronald, D. ,  KAHN, Theodore, W. ,  SIMS, Lynne, E.

IPC分类号： C12N15/31 ,  C12N15/63 ,  C12N15/82 ,  C07K14/325 ,  A01H5/00 ,  A01H5/10 ,  A01N63/00 ,  A01N63/02

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding delta-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.