公开(公告)号：EP1531664A2

公开(公告)日：2005-05-25

申请号：EP03742192.2

申请日：2003-06-25

申请人： E.I. du Pont de Nemours and Company

发明人： ABAD, Andre, R. ,  FLANNAGAN, Ronald, D. ,  HERRMANN, Rafael ,  KAHN, Theodore, W. ,  LU, Albert, Laurence ,  MCCUTCHEN, Billy Fred ,  PRESNAIL, James, K. ,  WONG, James, F., H. ,  YU, Cao-Guo

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/32 ,  C07K14/325 ,  C12Q1/25

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding delta-endotoxins having pesticidal activity against insect pests. The invention further provides mutagenized nucleic acids that have been modified to encode pesticidal polypeptides such as endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins that may be optimized as well as pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

公开(公告)号：EP1709068A2

公开(公告)日：2006-10-11

申请号：EP04814870.4

申请日：2004-12-20

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. du Pont de Nemours and Company

发明人： ABAD, André ,  FLANNAGAN, Ronald, D. ,  HERRMANN, Rafael ,  LU, Albert ,  MCCUTCHEN, Billy, F. ,  PRESNAIL, James, K. ,  RICE, Janet, A. ,  WONG, James, F. ,  YU, Cao-Guo

IPC分类号： C07K14/325 ,  C12N9/64 ,  C12N15/82 ,  A01H5/02

CPC分类号： C12N15/8286 ,  C12N9/641 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.