公开(公告)号：EP1794319A4

公开(公告)日：2009-10-21

申请号：EP05802610

申请日：2005-09-30

申请人： EPIGENOMICS AG

发明人： BALLHAUSE MATTHIAS ,  BERLIN KURT ,  DIETRICH DIMO ,  KLUTH ANTJE ,  SCHUSTER MATTHIAS ,  WAGNER UTE ,  WASSERKORT REINHOLD ,  ZIEBARTH HEIKE

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  C12N15/1003 ,  C12Q2527/127 ,  C12Q2523/125 ,  C12Q2521/531 ,  C12Q2547/107

摘要： Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, an are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable.

公开(公告)号：EP2014776A2

公开(公告)日：2009-01-14

申请号：EP08012765

申请日：2001-04-06

申请人： EPIGENOMICS AG

发明人： OLEK ALEXANDER ,  PIEPENBROCK CHRISTIAN ,  BERLIN KURT

IPC分类号： C12Q1/68 ,  G01N27/62 ,  A61K31/711 ,  A61K48/00 ,  A61P7/04 ,  A61P9/10 ,  A61P11/06 ,  A61P13/12 ,  A61P29/00 ,  A61P35/00 ,  B01J19/00 ,  C07K14/46 ,  C07K14/47 ,  C07K14/82 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/48 ,  G01N33/483 ,  G01N33/53 ,  G01N33/566 ,  G01N37/00

CPC分类号： C12Q1/6883 ,  C07K14/4703 ,  C07K14/82 ,  C12Q1/6886 ,  C12Q2600/154 ,  C12Q2600/156

摘要： The present invention relates to the chemically modified genomic sequences of genes associated with DNA transcription, to oligonucleotides and/or PNA-oligomers for detecting the cytosine methylation state of genes associated with DNA transcription which are directed against the sequence, as well as to a method for ascertaining genetic and/or epigenetic parameters of genes associated with DNA transcription.

摘要翻译： 本发明涉及与DNA转录相关的基因的化学修饰的基因组序列，涉及用于检测针对该序列的与DNA转录相关的基因的胞嘧啶甲基化状态的寡核苷酸和/或PNA-寡聚体，以及一种方法 用于确定与DNA转录相关的基因的遗传和/或表观遗传参数。