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公开(公告)号：EP1994145A2
公开(公告)日：2008-11-26
申请号：EP07722918.5
申请日：2007-02-23
申请人： ERA Biotech, S.A.
发明人： HEIFETZ, Peter Bernard , LLOMPART ROYO, Blanca , MARZÁBAL LUNA, Pablo , BASTIDA VIRGILI, Miriam , LUDEVID MUGICA, María Dolores , TORRENT QUETGLAS, Margarita , O CONNOR, Kevin James , PALLISSE BERGWERF, Roser , LLOP, Mª Inmaculada
CPC分类号： A61K39/00 , A61K9/0019 , A61K9/0053 , C07K14/415 , C07K14/425 , C07K14/43504 , C07K14/485 , C07K14/61 , C12N9/6424 , C12N9/6475 , C12N15/62 , C12N15/8257 , C12N2799/026 , C12P21/02 , C12Y304/21009
摘要： A fusion protein that is expressed in a recombinant protein body-like assembly (RPBLA) in host eukaryotic cells and organisms is disclosed. More particularly, a biologically active polypeptide fused to a protein sequence that mediates the induction of RPBLA formation is expressed and accumulated in host cells after transformation with an appropriate vector. The eukaryotic host cell does not produce protein bodies in the absence of the fusion protein. Methods for preparing and using the RPBLAs and the fusion protein are also disclosed, as are nucleic acid molecules that encode the fusion proteins.
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公开(公告)号：EP1819820A1
公开(公告)日：2007-08-22
申请号：EP05816137.3
申请日：2005-11-29
申请人： ERA Biotech, S.A.
发明人： LUDEVID MUGICA, María Dolores , BASTIDA VIRGILI, Miriam , LLOMPART ROYO, Blanca , MARZÁBAL LUNA, Pablo , TORRENT QUETGLAS, Margarita
CPC分类号： C07K14/61 , C07K14/415 , C07K14/425 , C07K14/485 , C07K14/585 , C07K2319/00 , C07K2319/04 , C07K2319/735 , C12N15/8221 , C12N15/8257
摘要： A method for purifying a recombinant fusion protein that is expressed as recombinant protein body-like assemblies (RPBLAs) in host cells is disclosed in which an aqueous homogenate of transformed host cells that express a fusion protein as RPBLAs having a predetermined density is provided. Regions of different density are formed in the homogenate to provide a region that contains a relatively enhanced concentration of the RPBLAs and a region that contains a relatively depleted concentration of the RPBLAs. The RPBLAs-depleted region is separated from the region of relatively enhanced concentration of RPBLAs, thereby purifying said fusion protein. The region of relatively enhanced concentration of RPBLAs can thereafter be collected as desired.
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公开(公告)号：EP2576797A1
公开(公告)日：2013-04-10
申请号：EP11723923.6
申请日：2011-05-30
申请人： ERA Biotech, S.A.
发明人： MARZÁBAL LUNA, Pablo , LLOP, Mª Inmaculada , LUDEVID MUGICA, María Dolores , LLOMPART ROYO, Blanca , MINU, Joseph , TORRENT QUETGLAS, Margarita , BASTIDA VIRGILI, Miriam
IPC分类号： C12N15/82
CPC分类号： C12N15/8221 , C07K14/415 , C12N15/8216 , C12N15/8257
摘要： Polypeptide sequences for inducing recombinant protein bodies are described. The sequences comprise a polyproline II (PPII) structure between two cysteine residues on either end and an ER signal sequence Recombinant protein bodies are useful for protein production because they allow for simple and efficient purification of high quantities of recombinant protein.In addition, other methods of using recombinant protein bodies, for example, in vaccination, are also described.
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公开(公告)号：EP1994145B1
公开(公告)日：2012-04-04
申请号：EP07722918.5
申请日：2007-02-23
申请人： ERA Biotech, S.A.
发明人： HEIFETZ, Peter Bernard , LLOMPART ROYO, Blanca , MARZÁBAL LUNA, Pablo , BASTIDA VIRGILI, Miriam , LUDEVID MUGICA, María Dolores , TORRENT QUETGLAS, Margarita , O CONNOR, Kevin James , PALLISSE BERGWERF, Roser , LLOP, Mª Inmaculada
CPC分类号： A61K39/00 , A61K9/0019 , A61K9/0053 , C07K14/415 , C07K14/425 , C07K14/43504 , C07K14/485 , C07K14/61 , C12N9/6424 , C12N9/6475 , C12N15/62 , C12N15/8257 , C12N2799/026 , C12P21/02 , C12Y304/21009
摘要： A fusion protein that is expressed in a recombinant protein body-like assembly (RPBLA) in host eukaryotic cells and organisms is disclosed. More particularly, a biologically active polypeptide fused to a protein sequence that mediates the induction of RPBLA formation is expressed and accumulated in host cells after transformation with an appropriate vector. The eukaryotic host cell does not produce protein bodies in the absence of the fusion protein. Methods for preparing and using the RPBLAs and the fusion protein are also disclosed, as are nucleic acid molecules that encode the fusion proteins.
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