公开(公告)号：EP1994145A2

公开(公告)日：2008-11-26

申请号：EP07722918.5

申请日：2007-02-23

申请人： ERA Biotech, S.A.

发明人： HEIFETZ, Peter Bernard ,  LLOMPART ROYO, Blanca ,  MARZÁBAL LUNA, Pablo ,  BASTIDA VIRGILI, Miriam ,  LUDEVID MUGICA, María Dolores ,  TORRENT QUETGLAS, Margarita ,  O CONNOR, Kevin James ,  PALLISSE BERGWERF, Roser ,  LLOP, Mª Inmaculada

IPC分类号： C12N5/10 ,  C12N15/79 ,  A61K39/00

CPC分类号： A61K39/00 ,  A61K9/0019 ,  A61K9/0053 ,  C07K14/415 ,  C07K14/425 ,  C07K14/43504 ,  C07K14/485 ,  C07K14/61 ,  C12N9/6424 ,  C12N9/6475 ,  C12N15/62 ,  C12N15/8257 ,  C12N2799/026 ,  C12P21/02 ,  C12Y304/21009

摘要： A fusion protein that is expressed in a recombinant protein body-like assembly (RPBLA) in host eukaryotic cells and organisms is disclosed. More particularly, a biologically active polypeptide fused to a protein sequence that mediates the induction of RPBLA formation is expressed and accumulated in host cells after transformation with an appropriate vector. The eukaryotic host cell does not produce protein bodies in the absence of the fusion protein. Methods for preparing and using the RPBLAs and the fusion protein are also disclosed, as are nucleic acid molecules that encode the fusion proteins.

公开(公告)号：EP1994145B1

公开(公告)日：2012-04-04

申请号：EP07722918.5

申请日：2007-02-23

申请人： ERA Biotech, S.A.

发明人： HEIFETZ, Peter Bernard ,  LLOMPART ROYO, Blanca ,  MARZÁBAL LUNA, Pablo ,  BASTIDA VIRGILI, Miriam ,  LUDEVID MUGICA, María Dolores ,  TORRENT QUETGLAS, Margarita ,  O CONNOR, Kevin James ,  PALLISSE BERGWERF, Roser ,  LLOP, Mª Inmaculada

IPC分类号： C12N5/10 ,  C12N15/79 ,  A61K39/00

CPC分类号： A61K39/00 ,  A61K9/0019 ,  A61K9/0053 ,  C07K14/415 ,  C07K14/425 ,  C07K14/43504 ,  C07K14/485 ,  C07K14/61 ,  C12N9/6424 ,  C12N9/6475 ,  C12N15/62 ,  C12N15/8257 ,  C12N2799/026 ,  C12P21/02 ,  C12Y304/21009

摘要： A fusion protein that is expressed in a recombinant protein body-like assembly (RPBLA) in host eukaryotic cells and organisms is disclosed. More particularly, a biologically active polypeptide fused to a protein sequence that mediates the induction of RPBLA formation is expressed and accumulated in host cells after transformation with an appropriate vector. The eukaryotic host cell does not produce protein bodies in the absence of the fusion protein. Methods for preparing and using the RPBLAs and the fusion protein are also disclosed, as are nucleic acid molecules that encode the fusion proteins.

公开(公告)号：EP2761006A1

公开(公告)日：2014-08-06

申请号：EP12772263.5

申请日：2012-09-28

申请人： ERA Biotech, S.A.

发明人： PALLISSE BERGWERF, Roser ,  SCHMIDT, Stefan Robert ,  MARCO FELIU, Dídac ,  CARVAJAL VALLEJOS, Patricia Karina

IPC分类号： C12N15/62 ,  C12N15/82 ,  C07K14/195 ,  C12N1/21

CPC分类号： C12P21/02 ,  C07K14/195 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/92 ,  C12N9/0006 ,  C12N9/0093 ,  C12N9/90 ,  C12N15/62 ,  C12N15/82 ,  C12P21/06

摘要： The present invention relates generally to robust split inteins. The split inteins described herein are active over a large temperature range, including temperatures as low as 0 ºC, over a wide pH range, and in the presence of chaotropic salts. The split inteins also show high tolerance to sequence variability in fused heterologous polypeptides and therefore are useful in protein purification and engineering techniques.