公开(公告)号：EP3158316A1

公开(公告)日：2017-04-26

申请号：EP15728576.8

申请日：2015-06-16

申请人： Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris

发明人： NERIN, Philippe Marc ,  BREMOND, Nicolas ,  BIBETTE, Jérôme ,  BAUDRY, Jean ,  GILES, Rory

IPC分类号： G01N15/10 ,  G01N15/14 ,  G01N15/00

CPC分类号： G01N15/14 ,  G01N15/1012 ,  G01N15/1459 ,  G01N2015/003 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1018

摘要： The present invention relates to the use of a solution of liquid particles in suspension for the control, calibration and/or performance of physical, in particular optical, measurements in a flow cytometry device for analysis of biological cells, said solution comprising liquid particles (21) of a first liquid phase dispersed in a second liquid phase, said liquid particles (21) having physical, chemical and/or biochemical properties that enable the attainment of physical measurements in said flow cytometry device similar to measurements obtained with biological cells, and said liquid particles (21), having a diameter for which the coefficient of variation within the solution of liquid particles in suspension is less than 10%. The invention also relates to a measurement method and a flow cytometry device implementing the solution of liquid particles in suspension.

摘要翻译： 本发明涉及悬浮液中的液体颗粒溶液用于流式细胞仪分析生物细胞的物理特别是光学测量的控制，校准和/或性能的用途，所述溶液包含液体颗粒（21 ）分散在第二液相中的第一液相，所述液体颗粒（21）具有物理，化学和/或生物化学性质，所述物理，化学和/或生物化学性质使得能够在所述流式细胞术装置中获得与用生物细胞获得的测量相似的物理测量结果，并且所述 液体颗粒（21）具有直径，液体颗粒悬浮液溶液内的变化系数小于10％。 本发明还涉及实施悬浮液中液体颗粒溶液的测量方法和流式细胞仪。