SYSTEMS AND METHODS FOR DIAGNOSING A FLUIDICS SYSTEM AND DETERMINING DATA PROCESSING SETTINGS FOR A FLOW CYTOMETER
    2.
    发明公开
    SYSTEMS AND METHODS FOR DIAGNOSING A FLUIDICS SYSTEM AND DETERMINING DATA PROCESSING SETTINGS FOR A FLOW CYTOMETER 审中-公开
    系统和方式,一种是流体系统的诊断及数据处理设置的流式细胞仪的测定

    公开(公告)号:EP3114458A1

    公开(公告)日:2017-01-11

    申请号:EP15703722.7

    申请日:2015-02-03

    IPC分类号: G01N15/14

    摘要: The present set of embodiments relates to systems and methods for diagnosing a fluidics system and determining data processing settings for a flow cytometer. Systems and methods for diagnosing a fluidics system require accurate measurement and interpretation of fluctuations within the fluid delivery system. Systems and methods for determining data processing settings require an accurate measurement of peak times among various channels and being able to adjust time delay settings wherein peak time is the measurement of time elapsed from the beginning of the data collection time window to the highest peak in the window.

    摘要翻译: 本组实施例中涉及的系统和方法用于诊断流体系统和确定性采矿数据处理设置流式细胞仪。 用于诊断流体系统的系统和方法需要精确的测量和所述流体输送系统内的波动的解释。系统和用于确定性挖掘数据处理设置的方法需要对的各通道之间高峰时间精确测量,并能够调整时间延迟设置worin峰值时间 是时间从数据采集时间窗口在窗口中最高峰的开始经过测量。

    XENOGRAFT TISSUE CONTROL FOR HISTOLOGY
    6.
    发明公开
    XENOGRAFT TISSUE CONTROL FOR HISTOLOGY 审中-公开
    异种移植ALS REFEERENZPROBE组织学

    公开(公告)号:EP1877784A2

    公开(公告)日:2008-01-16

    申请号:EP06752070.0

    申请日:2006-05-01

    摘要: An embodiment of the invention is a method of using a xenograft as a control tissue for histology, comprising staining both a patient and a xenograft-derived control sample under substantially similar staining conditions, and assessing the staining outcomes of the two to determine whether the stain was effective for the patient sample. A xenograft has never been used before in histology as a control, as far as the inventors know. The result of using a xenograft as a control is surprisingly advantageous. First, the cell lines grow and differentiate similarly to a human, taking on the general morphology of a real tissue sample. Second, because the same transformed cell line can be grown limitless times in SCID mice, the xenograft control is highly reproducible, leading to a consistent artificial control that is highly manufacturable and subject to genetic manipulation so that antigens or genetic elements may be embedded in the tissue. Another embodiment of the invention is directed generally to a method of making a tissue control substrate, comprising growing a xenograft from a mammalian transformed cell line in a host animal, removing the xenograft from the host animal, processing the xenograft thereby embedding the xenograft tissue in an embedding medium, and finally affixing the embedded xenograft sample onto a substrate. The substrate is generally a microscope slide. The xenograft control slide can then be stained side-by-side with a specimen sample in an automated slide stainer, and act as a control against which the staining quality can be compared. The xenograft control can also be used as a manual staining control. Determining whether the staining was effective for the patient specimen comprises judging the staining intensity of the xenograft control sample to determine if the expected degree and type of staining were realized in the control. If the expected type (nuclear, membranous, or cytoplasmic) and degree (0-4 scale) of staining are realized during the run, then the xenograft control indicates the staining process and reagents are working properly, and so the result in the patient specimen can be trusted. A further embodiment of the invention is a xenograft-derived control slide for histochemical use, comprising at least one xenograft control sample prepared for histological use, and a sample slide upon which the at least one xenograft control sample is affixed.

    MEASURING THE VELOCITY OF SMALL MOVING OBJECTS SUCH AS CELLS
    8.
    发明公开
    MEASURING THE VELOCITY OF SMALL MOVING OBJECTS SUCH AS CELLS 审中-公开
    测量小MOVING的速度随着细胞的对象,例如

    公开(公告)号:EP1328894A1

    公开(公告)日:2003-07-23

    申请号:EP01970578.9

    申请日:2001-08-24

    申请人: Amnis Corporation

    IPC分类号: G06K9/00

    摘要: Frequency domain velocity measurements and time domain velocity measurements are made using light from cells or other objects (18a). An optical grating (46) is used to modulate the light from an object so that it has a frequency proportional to the velocity of the object. Depending upon the embodiment, the pitch of the optical grating is uniform or varying. The modulated light is detected and various signal processing techniques, such as a Fast Fourier Transform function or processing in the time domain, are used to determinate an indication of the velocity of the object. Preferably, the indication of the velocity is applied in producing a timing signal employed for synchronization of an image of the object and an detector signal in an optical analysis system that uses a time delay integration detector to determine characteristics of the object in response to light from the object.