摘要:
The present invention concerns a modified oligonucleotide having two or a plurality of thiol functions, capable of being immobilised on a gold surface or on a grafted surface, in particular a surface comprising at least one carbon-carbon double bond or one carbon-carbon triple bond or halogen acetamide functions, preferably maleimide or acrylamide functions. The invention also concerns a method for detecting a nucleic acid in a biological sample comprising a step of detecting hybridisation between a modified oligonucleotide and an amplified target nucleic acid from the biological sample. The invention more specifically concerns a method for detecting genotyping or sequencing of a pathogenic organism, preferably a virus.
摘要:
The present invention relates to a thiol compound suitable for forming a chain of oligomers that can be grafted to an oligonucleotide. The invention also relates to an oligonucleotide grafted by such a compound, thus having one or more thiol functions, suitable for being immobilized on a gold surface or on a grafted surface.