公开(公告)号：EP4390368A1

公开(公告)日：2024-06-26

申请号：EP22383262.7

申请日：2022-12-22

申请人： European Molecular Biology Laboratory ,  Fundació Centre de Regulació Genòmica

发明人： Cotterell, James ,  Sharpe, James ,  Swoger, James ,  Robert-Moreno, Alexandre

IPC分类号： G01N15/14 ,  G06T7/73 ,  G01N15/10

CPC分类号： G01N15/1425 ,  G01N15/1429 ,  G01N15/1434 ,  G01N2015/144720130101 ,  G01N2015/100620130101 ,  G06T7/73 ,  G06T2207/1005620130101 ,  G06T2207/1006420130101 ,  G06T2207/3002420130101 ,  G01N15/1433

摘要： A method for determining spatial location of one or more biological components (12) in a biological sample (10) is proposed. The method comprises optically encoding (S100) the biological components (12) in the biological sample (10) with a plurality of fluorophores (31, 32, 33, 34) to create a combination of active fluorophore concentrations indicative of the spatial location of the one or more biological components (12); dissociating (S200) the biological components (12) from the biological sample (10) to create dissociated biological components (12) of the biological components (12); and - optically decoding (S300) by measuring the fluorescence (S310) of the dissociated biological components (12) and mapping (S320) to the spatial location of the biological components (12) in the non-dissociated sample (10).