公开(公告)号：EP0726958B1

公开(公告)日：2002-03-06

申请号：EP94931165.8

申请日：1994-10-26

申请人： FUJISAWA PHARMACEUTICAL CO., LTD.

发明人： NIWA, Mineo ,  SAITO, Yoshimasa ,  FUJIMURA, Takao ,  ISHII, Yoshinori ,  NOGUCHI, Yuji

IPC分类号： C12N15/55 ,  C12N9/80 ,  C12N15/70 ,  C12N1/21 ,  C12P35/02

CPC分类号： C12P35/02 ,  C12N9/80

摘要： A mutant CC acylase wherein at least one amino acid at the A1a , Met , Ser , Met , Glu , Met , Met , or Met position of the animo acid sequence of the native CC acylase is replaced by a different amino acid, a DNA coding therefor, an expression vector containing the said DNA, a microorganism transformed with the said expression vector, the production of the CC acylase by culturing the said transformant, and use thereof for the production of a compound. The mutant CC acylase of the invention has desirable properties in terms of enzymatic potency, alteration of pH profile, efficiency of processing, and the like.

公开(公告)号：EP0726958A1

公开(公告)日：1996-08-21

申请号：EP94931165.0

申请日：1994-10-26

申请人： FUJISAWA PHARMACEUTICAL CO., LTD.

发明人： NIWA, Mineo ,  SAITO, Yoshimasa ,  FUJIMURA, Takao ,  ISHII, Yoshinori ,  NOGUCHI, Yuji

IPC分类号： C12N15 ,  C12N1 ,  C12N9 ,  C12P35 ,  C12R1

CPC分类号： C12P35/02 ,  C12N9/80

摘要： A mutant CC acylase wherein at least one amino acid at the A1a?49, Met164, Ser166, Met174, Glu358, Met465, Met506¿, or Met750 position of the animo acid sequence of the native CC acylase is replaced by a different amino acid, a DNA coding therefor, an expression vector containing the said DNA, a microorganism transformed with the said expression vector, the production of the CC acylase by culturing the said transformant, and use thereof for the production of a compound. The mutant CC acylase of the invention has desirable properties in terms of enzymatic potency, alteration of pH profile, efficiency of processing, and the like.