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1.发明公开
公开(公告)号：EP0758679A1
公开(公告)日：1997-02-19
申请号：EP95909983.9
申请日：1995-02-24
CPC分类号： C12N9/0008 , C12N9/0006 , C12P7/60 , Y10S435/822
摘要： An expression vector containing both of a DNA coding for L-sorbose dehydrogenase and a DNA coding for L-sorbosone dehydrogenase; a transformant capable of producing 2-keto-L-gulonic acid (2KLGA) from D-sorbitol in a high yield and prepared by transforming with the above vector a microorganism which produces L-sorbose from D-sorbitol in a high yield and which has no or little activity of decomposing 2KLGA, or such a microorganism which has, in addition, no or little activity of producing L-idonic acid; and a process for producing 2KLGA by culturing the transformant in a medium containing D-sorbitol. The invention process enables 2KLGA useful for producing L-ascorbic acid to be produced readily in a large amount at a good efficiency by single culture.
摘要翻译： 含有编码L-山梨糖脱氢酶的DNA和编码L-山梨糖酮脱氢酶的DNA的表达载体; 能够以高产率从D-山梨糖醇生产2-酮-L-古洛糖酸（2KLGA）的转化体，并通过用上述载体以高产率从D-山梨醇转化产生L-山梨糖的微生物制备， 没有或很少分解2KLGA的活性，或者这样的微生物，另外没有或很少生产L-吉非糖的活性; 以及通过在含有D-山梨醇的培养基中培养转化体来生产2KLGA的方法。 本发明方法使得2KLGA能够通过单一培养以高效率大量容易地生产L-抗坏血酸。
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2.发明公开NOVEL L-SORBOSE DEHYDROGENASE AND NOVEL L-SORBOSONE DEHYDROGENASE OBTAINED FROM $i(GLUCONOBACTER OXYDANS) T-100 失效新型L-山梨糖脱氢酶和新型L-山梨糖酮脱氢酶获自$ i（葡糖杆菌氧化酶）T-100
公开(公告)号：EP0753575A1
公开(公告)日：1997-01-15
申请号：EP94908512.0
申请日：1994-03-08
IPC分类号： C12N9/04
CPC分类号： C12N9/0006 , Y10S435/822
摘要： A novel L-sorbose dehydrogenase (SDH) and a novel L-sorbosone dehydrogenase both derived from Gluconobacter oxydans T-100, a DNA which encodes the SDH and/or SNDH, an expression vector which contains the DNA, a host cell transformed by the expression vector and a process for producing the SDH and/or SNDH, which comprises culturing the host cell in a medium and recovering the SDH and/or SNDH from the resulting culture. The SDH and SNDH of the present invention are useful enzymes having preferable properties for the production of 2-keto-L-gulonic acid, as well as L-ascorbic acid. According to the production method of the present invention, the SDH and SNDH having such preferable properties can be produced in large amounts by genetic engineering.
摘要翻译： 一种新型L-山梨糖脱氢酶（SDH）和一种新型L-山梨糖酮脱氢酶都来源于氧化葡糖杆菌T-100，一种编码SDH和/或SNDH的DNA，一种含有该DNA的表达载体，一种由该转化的宿主细胞 表达载体和用于产生SDH和/或SNDH的方法，其包括在培养基中培养宿主细胞并从所得培养物中回收SDH和/或SNDH。 本发明的SDH和SNDH是有用的用于生产2-酮基-L-古洛糖酸和L-抗坏血酸的特性的酶。 根据本发明的生产方法，具有这种优选特性的SDH和SNDH可以通过基因工程大量生产。
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公开(公告)号：EP0726958B1
公开(公告)日：2002-03-06
申请号：EP94931165.8
申请日：1994-10-26
摘要： A mutant CC acylase wherein at least one amino acid at the A1a , Met , Ser , Met , Glu , Met , Met , or Met position of the animo acid sequence of the native CC acylase is replaced by a different amino acid, a DNA coding therefor, an expression vector containing the said DNA, a microorganism transformed with the said expression vector, the production of the CC acylase by culturing the said transformant, and use thereof for the production of a compound. The mutant CC acylase of the invention has desirable properties in terms of enzymatic potency, alteration of pH profile, efficiency of processing, and the like.
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公开(公告)号：EP0726958A1
公开(公告)日：1996-08-21
申请号：EP94931165.0
申请日：1994-10-26
摘要： A mutant CC acylase wherein at least one amino acid at the A1a?49, Met164, Ser166, Met174, Glu358, Met465, Met506¿, or Met750 position of the animo acid sequence of the native CC acylase is replaced by a different amino acid, a DNA coding therefor, an expression vector containing the said DNA, a microorganism transformed with the said expression vector, the production of the CC acylase by culturing the said transformant, and use thereof for the production of a compound. The mutant CC acylase of the invention has desirable properties in terms of enzymatic potency, alteration of pH profile, efficiency of processing, and the like.
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