公开(公告)号：EP1609867A1

公开(公告)日：2005-12-28

申请号：EP05000339.1

申请日：2005-01-10

申请人： Feng Chia University ,  Taichung District Agriculture Research and Extension Station, Council of Agriculture

发明人： Chao, Yun-Peng ,  Wang, Zei-Wen ,  Chen, Po-Ting ,  Chen, Yu-Hsin

IPC分类号： C12N15/63 ,  C12N15/67 ,  C12N15/71 ,  C12N15/72 ,  C12N15/73 ,  C12N5/10

CPC分类号： C12N9/0036 ,  C07K14/805 ,  C07K2319/00 ,  C12N15/67 ,  C12N15/70

摘要： Disclosed herein are nucleic acid constructs and expression vectors for enhancing the production of recombinant polypeptides/proteins, and methods for the massive production of recombinant polypeptides/proteins, in which a first nucleic acid sequence encoding thioredoxin and a second nucleic acid sequence encoding hemoglobin are cloned into a host cell, thereby enhancing the capability of the thus formed recombinant host cell in producing a selected gene product, and thereby assisting said recombinant host cell in relieving intracellular stress due to the overproduction of said gene product.

摘要翻译： 公开在用于大规模生产重组多肽/蛋白质，其中第一核酸序列编码硫氧还蛋白和第二核酸序列编码的血红蛋白被克隆的核酸构建体和用于增强生产重组多肽/蛋白质的表达载体，和方法 入宿主细胞中，从而提高了由此形成的重组宿主细胞的能力在制造将所选基因产物，从而辅助所述重组宿主细胞在胞内释放应力由于所述基因产物的生产过剩。

公开(公告)号：EP1609867B1

公开(公告)日：2010-09-29

申请号：EP05000339.1

申请日：2005-01-10

申请人： Feng Chia University ,  Taichung District Agriculture Research and Extension Station, Council of Agriculture

发明人： Chao, Yun-Peng ,  Wang, Zei-Wen ,  Chen, Po-Ting ,  Chen, Yu-Hsin

IPC分类号： C12N15/63 ,  C12N15/67 ,  C12N15/71 ,  C12N15/72 ,  C12N15/73 ,  C12N5/10

CPC分类号： C12N9/0036 ,  C07K14/805 ,  C07K2319/00 ,  C12N15/67 ,  C12N15/70