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1.发明公开The production of human serum albumin in bacillus 失效Herstellung von menschlichem Albumin-Serum in Bazillen。
公开(公告)号：EP0229712A2
公开(公告)日：1987-07-22
申请号：EP87300274.5
申请日：1987-01-13
申请人： GENEX CORPORATION
CPC分类号： C12N9/2417 , C07K14/765 , C07K2319/00 , C07K2319/02 , C07K2319/036 , C12N9/54 , C12N15/75
摘要： A protein which reacts with an antibody to human serum albumin (HSA) can be synthesized in a Bacillus host and secreted therefrom. A Bacillus host is transformed with an expression vector comprising a structural gene which encodes HSA fused in correct reading frame, either directly or via a linker sequence, to the expression elements and signal sequence coding region of a gene that can be recognized by the transcription and translation systems of the host, such that said expression elements control the expression of the HSA- encoding sequence. The transformed strain is grown in a culture medium and subjected to conditions under which the gene fusion is expressed. The resultant protein can be secreted from the Bacillus host into the culture medium.
摘要翻译： 与人血清白蛋白（HSA）的抗体反应的蛋白质可以在芽孢杆菌宿主中合成并由其分泌。 用包含编码正确读框中HSA融合的结构基因的表达载体直接或通过接头序列转化到可被转录识别的基因的表达元件和信号序列编码区， 使得所述表达元件控制HSA编码序列的表达。 将转化的菌株在培养基中生长，并进行表达基因融合的条件。 所得蛋白质可以从芽孢杆菌宿主分泌到培养基中。
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公开(公告)号：EP0119083A3
公开(公告)日：1987-01-14
申请号：EP84301619
申请日：1984-03-09
申请人： GENEX CORPORATION
摘要： Disclosed is a series of hybrid bacterial plasmids which are useful for gene cloning in a wide variety of bacterial hosts. Also disclosed are microorganisms transformed by these plasmids.
摘要翻译： 公开了一系列可用于多种细菌宿主中的基因克隆的杂交细菌质粒。 还公开了由这些质粒转化的微生物。
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公开(公告)号：EP0119083A2
公开(公告)日：1984-09-19
申请号：EP84301619.7
申请日：1984-03-09
申请人： GENEX CORPORATION
摘要： Disclosed is a series of hybrid bacterial plasmids which are useful for gene cloning in a wide variety of bacterial hosts. Also disclosed are microorganisms transformed by these plasmids.
摘要翻译： 公开了一系列可用于多种细菌宿主中的基因克隆的杂交细菌质粒。 还公开了由这些质粒转化的微生物。
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