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公开(公告)号：EP1382963B1
公开(公告)日：2008-09-03
申请号：EP02722709.9
申请日：2002-04-19
发明人： HIRAMATSU, Yuka, c/o GL Sciences Incorporated , YAMASAKI, Kazuhiko, c/o GL Sciences Incorporated , FURUNO, Masahiro, c/o GL Sciences Incorporated
CPC分类号： G01N1/405 , B01D15/00 , B01D15/08 , B01J20/28042 , B01J20/28054 , B01J20/28092 , B01J2220/64 , B01L3/022 , B01L3/0275 , B01L2200/0631 , G01N1/40 , G01N30/18 , G01N2030/009
摘要： A method for extracting a component in a sample in solid phase in order to provide means effective for pretreating a sample being introduced to a chromatograph. The method comprises a step for providing a needle at the forward end of a syringe having a tube and a plunger slidable in the tube, forming a porous body of so-called monolithic structure in the needle at least over a proper length and the entire diameter and then inserting the needle into a sample, a step for passing the sample through the needle and holding a target component in the porous body, and a step for separating the target component from the interior of the porous body.
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公开(公告)号：EP1719816B1
公开(公告)日：2011-06-01
申请号：EP04710506.9
申请日：2004-02-12
发明人： ABUDOKIRIM, Abudogupur, GL Sciences Incorporated , OHIRA, Masayoshi, c/o GL Sciences Incorporated , OKUSA, Kensuke, c/o GL Sciences Incorporated , SETO, Nobuo, c/o GL Sciences Incorporated , FURUNO, Masahiro, c/o GL Sciences Incorporated
CPC分类号： C07H21/00 , C12N15/1006
摘要： A simplified mechanism and method for highly efficiently separating and purifying a nucleic acid (in particular a fragmental DNA) at a high reproducibility without resort to elution with a salt at a high concentration or elution/purification, thereby giving a fragment having a high purity. This mechanism, which aims at purifying a nucleic acid (in particular a fragmental DNA) with the use of a monolith structure, is provided with a monolith structure made of glass or silica, namely, a porous integral structure having small pores penetrating thorough from the top to the bottom and the penetrating pores being in a size fitting for the nucleic acid size of from 35 bp (mer) to 100 Kbp (mer).
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3.发明公开
公开(公告)号：EP1719816A1
公开(公告)日：2006-11-08
申请号：EP04710506.9
申请日：2004-02-12
发明人： ABUDOKIRIM, Abudogupur, GL Sciences Incorporated , OHIRA, Masayoshi, c/o GL Sciences Incorporated , OKUSA, Kensuke, c/o GL Sciences Incorporated , SETO, Nobuo, c/o GL Sciences Incorporated , FURUNO, Masahiro, c/o GL Sciences Incorporated
CPC分类号： C07H21/00 , C12N15/1006
摘要： A high-purity fragment is obtained by a simple mechanism and method for separating and purifying a nucleic acid, particularly fragment DNA, extremely efficiently and with a high reproducibility, wherein elution with a high-concentration salt is not performed and necessity of elution and purification is eliminated.
This mechanism is a mechanism for purifying a nucleic acid, particularly fragment DNA using a monolith structure formed with glass or silica, specifically, an integral porous body having an open structure with pores that communicate the upper end with the lower end, wherein through-pores corresponding to nucleic acid sizes of 35 bp (mer) to 100 Kbp (mer) are provided.摘要翻译： 通过简单的机制和方法，以非常有效和高重现性分离和纯化核酸，特别是片段DNA得到高纯度片段，其中不进行高浓度盐的洗脱，并且需要洗脱和纯化 被淘汰。 该机制是使用由玻璃或二氧化硅形成的整体结构，特别是具有开口结构的整体多孔体来纯化核酸，特别是片段DNA的机理，其具有使上端与下端连通的孔，其中通孔 对应于35bp（mer）至100Kbp（mer）的核酸大小。
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公开(公告)号：EP1382963A1
公开(公告)日：2004-01-21
申请号：EP02722709.9
申请日：2002-04-19
发明人： HIRAMATSU, Yuka, c/o GL Sciences Incorporated , YAMASAKI, Kazuhiko, c/o GL Sciences Incorporated , FURUNO, Masahiro, c/o GL Sciences Incorporated
CPC分类号： G01N1/405 , B01D15/00 , B01D15/08 , B01J20/28042 , B01J20/28054 , B01J20/28092 , B01J2220/64 , B01L3/022 , B01L3/0275 , B01L2200/0631 , G01N1/40 , G01N30/18 , G01N2030/009
摘要： An object of the present invention is to provide effective means for pre-treatment of a sample to be introduced in a chromatograph, and the present invention is a method for performing solid-phase extraction of a component present in a sample, which uses a syringe having a cylinder and a plunger slidable within the cylinder, a tip of the syringe being provided with a needle and the needle being provided with a porous body having an so-called monolithic structure along at least an appropriate length of the needle and across an overall diameter of the needle, including the steps of inserting the needle into the sample, passing the sample through the needle to retain an analyte within the porous body, and desorbing the retained analyte from the porous body.
摘要翻译： 本发明的目的在于提供用于对导入到色谱仪中的试样进行前处理的有效手段，本发明是一种使用注射器对试样中存在的成分进行固相萃取的方法 具有可在圆柱体内滑动的圆柱体和柱塞，注射器的尖端设置有针，并且针设置有多孔体，该多孔体具有沿着针的至少适当长度且横跨整体的所谓单体结构 包括将针插入样品，使样品通过针以将分析物保持在多孔体内以及从多孔体解吸保留的分析物的步骤。
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