专利检索 ap:("Gdanski Uniwersytet Medyczny") AND inv:"TRZONKOWSKl, Piotr" 第 1 页

1.

发明公开
METHOD FOR EX VIVO EXPANSION OF REGULATORY T CELLS 审中-公开

公开(公告)号：EP3402877A1

公开(公告)日：2018-11-21

申请号：EP16834056.0

申请日：2016-12-19

申请人： Gdanski Uniwersytet Medyczny

发明人： MAREK-TRZONKOWSKA, Natalia , TRZONKOWSKl, Piotr , MYSLIWIEC, Malgorzata

IPC分类号： C12N5/0783

CPC分类号： C12N5/0637 , C12N2500/00 , C12N2501/2302 , C12N2501/51 , C12N2501/515 , C12N2501/998 , C12N2523/00

摘要： The invention relates to a new method for in vitro expansion of CD4+CD25HighCD127−/LOWfoxP3+Tregs, wherein the process of Treg expansion takes place permanently or temporarily at a temperature below 37° C., optimally at a temperature of 33° C., the isolated Tregs are expanded in SCGM or X-vivo-20 medium supplemented with human serum or with foetal bovine serum, and magnetic beads coated with anti-CD3 and anti-CD28 antibodies at 1:1 (cell:bead) ratio and interleukin-2 are added to the culture.