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    METHOD FOR POLYCLONAL STIMULATION OF T CELLS BY MOBILE NANOMATRICES
    7.
    发明授权
    METHOD FOR POLYCLONAL STIMULATION OF T CELLS BY MOBILE NANOMATRICES 有权
    用移动纳米粒子多克隆刺激T细胞的方法

    公开(公告)号：EP2900809B1

    公开(公告)日：2017-05-31

    申请号：EP13766319.1

    申请日：2013-09-24

    申请人： Miltenyi Biotec GmbH

    发明人： SCHEFFOLD, Alexander ,  ASSENMACHER, Mario

    IPC分类号： C12N5/0783 ,  C12N5/00

    CPC分类号： C12N5/0637 ,  A61K35/17 ,  C12N5/0075 ,  C12N5/0636 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/998 ,  C12N2533/70 ,  C12N2539/00

    摘要： The present invention provides a method polyclonal stimulation of T cells, the method comprising contacting a population of T cells with a nanomatrix, the nanomatrix comprising a) a flexible matrix, wherein said matrix is of polymeric material; and b) attached to said polymeric flexible matrix one or more polyclonal stimulatory agents which provide activation signals to the T cells; thereby activating and inducing the T cells to proliferate; wherein the nanomatrix is 1 to 500 nm in size. At least one first and one second stimulatory agents are attached to the same or to separate flexible matrices. If the stimulatory agents are attached to separate beads, fine-tuning of nanomatrices for the stimulation of the T cells is possible.

    摘要翻译： 本发明提供了T细胞多克隆刺激的方法，所述方法包括使T细胞群与纳米基质接触，所述纳米基质包含a）柔性基质，其中所述基质为聚合物材料; 和b）连接到所述聚合物柔性基质上的一种或多种向T细胞提供激活信号的多克隆刺激剂; 从而激活并诱导T细胞增殖; 其中纳米基体的尺寸为1至500nm。 至少有一种第一种和第二种刺激剂附着在其上或分开柔性基质。 如果刺激剂附着于单独的珠上，可以微调纳米基质以刺激T细胞。

    CELL THERAPY FORMULATION METHOD AND COMPOSITION
    8.
    发明授权
    CELL THERAPY FORMULATION METHOD AND COMPOSITION 有权
    细胞疗法制剂方法和组合物

    公开(公告)号：EP1749090B1

    公开(公告)日：2017-05-17

    申请号：EP05724065.7

    申请日：2005-03-01

    申请人： Immunovative Therapies, Ltd.

    发明人： HAR-NOY, Michael

    IPC分类号： A61K35/14 ,  C12N5/0786

    CPC分类号： C12N5/0636 ,  A61K2039/5154 ,  A61K2039/57 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2531/00 ,  C12N2533/40

    摘要： A composition is described that comprises a treatment effective amount of cells, of which a portion are T-cells, wherein the T-cells have been harvested from cell culture and are thereby removed from activating stimuli in the culture and the harvested T-cells are formulated for infusion by a method comprising (1) contacting the T-cells with one or more agents that ligate a cell surface moiety on at least a portion of the T-cells, (2) cross-linking the one or more agents with biodegradable nanospheres or microspheres coated with a material capable of cross-linking the one or more agents, (3) suspending the T-cells, the one or more agents and the coated biodegradable nanospheres or microspheres in an infusion medium, and (4) packaging in a suitable container, wherein the T-cells are in an activated state in the medium, wherein the one or more agents are mouse-derived monoclonal antibodies, or fragments or genetically engineered derivatives thereof, and the material coating the nanospheres or microspheres is goat or sheep anti-mouse polyclonal antibodies. Methods for the preparation of the composition are also described.

    摘要翻译： 离体制备的T细胞从细胞培养条件收获并配制在适合输注的培养基中。 该制剂通过用一种或多种对T细胞表面部分具有反应性的试剂标记细胞来制备，所述试剂在交联时能够递送激活信号，并将标记的细胞与涂覆有能够交联的材料的生物可降解纳米球或微球混合 附着于T细胞表面部分的试剂。 或者，所述制剂可以通过将T细胞群体与涂覆有第一材料和一种或多种第二材料的生物可降解纳米球或微球体混合来制备。 第一材料结合第二材料并且第二材料对T细胞上的表面部分具有反应性并且第二材料与T细胞的相互作用导致T细胞的活化。 在任一种方法中，将T细胞和生物可降解球体的混合物悬浮于适于输注的介质中，并将混合物包装在容器中。

    METHODS FOR PREPARING T-CELLS FOR CELL THERAPY
    9.
    发明公开
    METHODS FOR PREPARING T-CELLS FOR CELL THERAPY 审中-公开
    VERFAHREN ZUR HERSTELLUNG VON T-ZELLEN ZUR ZELLTHERAPIE

    公开(公告)号：EP3067415A1

    公开(公告)日：2016-09-14

    申请号：EP16161764.2

    申请日：2005-02-24

    申请人： Immunovative Therapies, Ltd.

    发明人： HAR-NOY, Michael

    IPC分类号： C12N5/07 ,  A61K35/14

    CPC分类号： C12N5/0636 ,  A61K2039/5154 ,  A61K2039/57 ,  C12N2501/23 ,  C12N2501/40 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/998 ,  C12N2531/00 ,  C12N2533/40

    摘要： A biodegradable device for activating T-cells and a method for differentiation of T-cells using the device are disclosed, wherein, the device comprises an universal crosslinking agent comprising a biodegradable support coated with a first material, wherein the first material is capable of crosslinking more than one array of second materials, the second materials capable of binding T-cell surface moieties to deliver a signal to the T-cells, wherein each successive array of second materials has at least one second material different from the preceding array of second materials.

    摘要翻译： 公开了一种用于激活T细胞的可生物降解的装置和使用该装置分化T细胞的方法，其中，该装置包括通用交联剂，其包含涂覆有第一材料的可生物降解的载体，其中第一材料能够交联 多于一种第二材料阵列，所述第二材料能够结合T细胞表面部分以向T细胞递送信号，其中第二材料的每个连续阵列具有不同于前述第二材料阵列的至少一种第二材料 。