公开(公告)号：EP2872649B1

公开(公告)日：2018-02-21

申请号：EP13740457.0

申请日：2013-07-11

Applicant: Gen-Probe Incorporated

Inventor： DATTA, Somalee ,  BOWMAN, Brett ,  WANG, Xianqun ,  WANG, Mingjie

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/707 ,  C12Q1/6809 ,  C12Q2600/156 ,  C12Q2600/16 ,  G06F19/18 ,  C12Q2535/122 ,  C12Q2537/165

Abstract: Disclosed are methods for detecting a minority genotype of a target nucleic acid. The disclosed method generally includes the steps of (a) deep sequencing at least a portion of the target nucleic acid; (b) using the deep sequencing results of (a) to detect the presence of variant nucleobases at one or more nucleotide reference positions within the target nucleic acid; (c) using the variant detection results generated in step (b) to perform a statistical analysis of whether the variants are significant; and (d) using the variant detection and variant significance results generated in steps (b) and (c) to perform a statistical analysis of whether a subset of sequences together exhibit a common set of significant variants.

公开(公告)号：EP3339448B1

公开(公告)日：2020-03-25

申请号：EP18157235.5

申请日：2013-07-11

Applicant: Gen-Probe Incorporated

Inventor： DATTA, Somalee ,  BOWMAN, Brett ,  WANG, Xianqun ,  WANG, Mingjie

IPC: C12Q1/68 ,  C12Q1/70

公开(公告)号：EP3339448A1

公开(公告)日：2018-06-27

申请号：EP18157235.5

申请日：2013-07-11

Applicant: Gen-Probe Incorporated

Inventor： DATTA, Somalee ,  BOWMAN, Brett ,  WANG, Xianqun ,  WANG, Mingjie

IPC: C12Q1/68 ,  C12Q1/70

Abstract: Disclosed are methods for detecting a minority genotype of a target nucleic acid. The disclosed method generally includes the steps of (a) deep sequencing at least a portion of the target nucleic acid; (b) using the deep sequencing results of (a) to detect the presence of variant nucleobases at one or more nucleotide reference positions within the target nucleic acid; (c) using the variant detection results generated in step (b) to perform a statistical analysis of whether the variants are significant; and (d) using the variant detection and variant significance results generated in steps (b) and (c) to perform a statistical analysis of whether a subset of sequences together exhibit a common set of significant variants.

公开(公告)号：EP2872649A1

公开(公告)日：2015-05-20

申请号：EP13740457.0

申请日：2013-07-11

Applicant: Gen-Probe Incorporated

Inventor： DATTA, Somalee ,  BOWMAN, Brett ,  WANG, Xianqun ,  WANG, Mingjie

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/707 ,  C12Q1/6809 ,  C12Q2600/156 ,  C12Q2600/16 ,  G06F19/18 ,  C12Q2535/122 ,  C12Q2537/165

Abstract: Disclosed are methods for detecting a minority genotype of a target nucleic acid. The disclosed method generally includes the steps of (a) deep sequencing at least a portion of the target nucleic acid; (b) using the deep sequencing results of (a) to detect the presence of variant nucleobases at one or more nucleotide reference positions within the target nucleic acid; (c) using the variant detection results generated in step (b) to perform a statistical analysis of whether the variants are significant; and (d) using the variant detection and variant significance results generated in steps (b) and (c) to perform a statistical analysis of whether a subset of sequences together exhibit a common set of significant variants.

Abstract translation: 公开了用于检测目标核酸的少数基因型的方法。 所公开的方法通常包括以下步骤：（a）对目标核酸的至少一部分进行深度测序; （b）使用（a）的深测序结果来检测靶核酸内的一个或多个核苷酸参考位置处的变体核碱基的存在; （c）使用步骤（b）中产生的变体检测结果来进行变异是否显着的统计分析; 和（d）使用在步骤（b）和（c）中产生的变体检测和变体显着性结果来执行序列子集是否一起显示共同的一组重要变体的统计分析。