公开(公告)号：EP2504449B1

公开(公告)日：2016-03-23

申请号：EP10784391.4

申请日：2010-11-19

申请人： Gen9, Inc.

发明人： RAMU, Senthil ,  JACOBSON, Joseph ,  CHU, Larry Li-Yang

IPC分类号： C12Q1/68

CPC分类号： C12N15/1058 ,  C12Q1/6848 ,  C12Q2521/514 ,  C12Q2565/537

公开(公告)号：EP4039363A1

公开(公告)日：2022-08-10

申请号：EP22150372.5

申请日：2011-11-10

申请人： Gen9, Inc.

发明人： JACOBSON, Joseph ,  HUDSON, Michael E. ,  CHU, Larry Li-Yang

IPC分类号： B01J19/00 ,  C40B50/14 ,  B82Y30/00

摘要： Methods and devices are provided for preparing a protein array having a plurality of proteins. In one embodiment, the method includes providing a plurality of nucleic acids each having a predefined sequence and expressing in vitro a plurality of proteins from the plurality of nucleic acids. In another embodiment, protein arrays having a solid surface and a microvolume are also provided. The solid surface can have a plurality of anchor oligonucleotides capable of hybridizing with a plurality of nucleic acids. The microvolume can cover each of the plurality of anchor oligonucleotides and can be configured to produce a polypeptide from each of the plurality of nucleic acids.

公开(公告)号：EP3597771A1

公开(公告)日：2020-01-22

申请号：EP19174924.1

申请日：2010-11-19

申请人： Gen9, Inc.

发明人： RAMU, Senthil ,  JACOBSON, Joseph ,  CHU, Larry Li-Yang

IPC分类号： C12Q1/68

摘要： Methods and apparatus relate to reduction of sequence errors generated during synthesis of nucleic acids on a microarray chip. The error reduction can include synthesis of complementary strands (to template strands), using a short universal primer complementary to the template strands and polymerase. Heteroduplex can be formed by melting and re-annealing complementary strands and template strands. The heteroduplexes containing a mismatch can be recognized and cleaved by a mismatch endonuclease. The mismatch-containing cleaved heteroduplexes can be removed from the microarray chip using a global buffer exchange. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.

公开(公告)号：EP2504449A1

公开(公告)日：2012-10-03

申请号：EP10784391.4

申请日：2010-11-19

申请人： Gen9, Inc.

发明人： RAMU, Senthil ,  JACOBSON, Joseph ,  CHU, Larry Li-Yang

IPC分类号： C12Q1/68

CPC分类号： C12N15/1058 ,  C12Q1/6848 ,  C12Q2521/514 ,  C12Q2565/537

摘要： Methods and apparatus relate to reduction of sequence errors generated during synthesis of nucleic acids on a microarray chip. The error reduction can include synthesis of complementary stands (to template strands), using a short universal primer complementary to the template strands and polymerase. Heteroduplex can be formed be melting and re-annealing complementary stands and template strands. The heteroduplexes containing a mismatch can be recognized and cleaved by a mismatch endonuclease. The mismatch-containing cleaved heteroduplexes can be removed from the microarray chip using a global buffer exchange. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.

公开(公告)号：EP3085791A1

公开(公告)日：2016-10-26

申请号：EP16155732.7

申请日：2010-11-19

申请人： Gen9, Inc.

发明人： RAMU, Senthil ,  JACOBSON, Joseph ,  CHU, Larry Li-Yang

IPC分类号： C12Q1/68

CPC分类号： C12N15/1058 ,  C12Q1/6848 ,  C12Q2521/514 ,  C12Q2565/537

摘要： Methods and apparatus relate to reduction of sequence errors generated during synthesis of nucleic acids on a microarray chip. The error reduction can include synthesis of complementary strands (to template strands), using a short universal primer complementary to the template strands and polymerase. Heteroduplex can be formed by melting and re-annealing complementary strands and template strands. The heteroduplexes containing a mismatch can be recognized and cleaved by a mismatch endonuclease. The mismatch-containing cleaved heteroduplexes can be removed from the microarray chip using a global buffer exchange. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.

摘要翻译： 方法和装置涉及减少在微阵列芯片上合成核酸期间产生的序列错误。 错误减少可以包括使用与模板链和聚合酶互补的短通用引物合成互补链（模板链）。 异源双链体可以通过融合并重新退火互补链和模板链来形成。 含有错配的异源双链体可被错配核酸内切酶识别和切割。 可以使用全局缓冲液交换从微阵列芯片去除含错配的裂解的异源双链体。 由其产生的无错合成核酸可用于多种应用，包括生物燃料和增值药物产品的合成。

公开(公告)号：EP2637780A1

公开(公告)日：2013-09-18

申请号：EP11788706.7

申请日：2011-11-10

申请人： Gen9, Inc.

发明人： JACOBSON, Joseph ,  HUDSON, Mike ,  CHU, Larry Li-Yang

IPC分类号： B01J19/00 ,  C40B50/14 ,  B82Y30/00

CPC分类号： C12N15/1086 ,  B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00509 ,  B01J2219/00531 ,  B01J2219/00596 ,  B01J2219/00621 ,  B01J2219/00644 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B50/14

摘要： Methods and devices are provided for preparing a protein array having a plurality of proteins. In one embodiment, the method includes providing a plurality of nucleic acids each having a predefined sequence and expressing in vitro a plurality of proteins from the plurality of nucleic acids. In another embodiment, protein arrays having a solid surface and a microvolume are also provided. The solid surface can have a plurality of anchor oligonucleotides capable of hybridizing with a plurality of nucleic acids. The microvolume can cover each of the plurality of anchor oligonucleotides and can be configured to produce a polypeptide from each of the plurality of nucleic acids.

摘要翻译： 提供了用于制备具有多种蛋白质的蛋白质阵列的方法和装置。 在一个实施方案中，所述方法包括提供多个核酸，其各自具有预定义的序列并且在体外表达来自所述多个核酸的多种蛋白质。 在另一个实施方案中，还提供了具有固体表面和微量体积的蛋白质阵列。 固体表面可以具有能够与多个核酸杂交的多个锚定寡核苷酸。 微体积可以覆盖多个锚定寡核苷酸中的每一个，并且可以被配置为从多个核酸中的每一个生成多肽。