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公开(公告)号：EP2925371B1
公开(公告)日：2019-04-03
申请号：EP13858426.3
申请日：2013-11-26
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公开(公告)号：EP2652175B1
公开(公告)日：2017-09-20
申请号：EP11848353.6
申请日：2011-12-14
IPC分类号： C25B7/00 , G01N27/447 , C07H21/00
CPC分类号： C12N15/101
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公开(公告)号：EP3164510A1
公开(公告)日：2017-05-10
申请号：EP15816060.6
申请日：2015-06-22
发明人： NELSON, John Richard , MOORE, David Roger , DUTHIE, Robert Scott , MISNER, Matthew Jeremiah , GROSSMANN, Gregory Andrew , DEES, Elizabeth Marie , SPOONER, Patrick McCoy , KVAM, Erik Leeming , BURNS, Andrew Arthur Paul , WATKINS, Vicki Herzl
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12Q1/6834 , C12Q2565/625
摘要： A method is provided herein, the method includes : applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.
摘要翻译： 本文提供了一种方法，该方法包括：将包含靶核酸的样品施加至基板的样品施加区域; 以及使核酸扩增反应混合物在一段长度的底物上流过样品应用区以扩增形成核酸扩增产物的靶核酸; 其中所述具有第一分子量的靶核酸基本上固定在所述样品施加区域，并且其中具有第二分子量的所述扩增产物从所述样品施加区域迁移出来。 还提供了关联的设备。
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公开(公告)号：EP2925371A1
公开(公告)日：2015-10-07
申请号：EP13858426.3
申请日：2013-11-26
CPC分类号： G01N1/312 , A01N1/0242 , A01N1/0284 , B01L3/5023 , B01L3/5088 , B01L7/00 , B01L2200/0678 , B01L2300/0825 , B01L2300/126 , B01L2300/1877 , G01N1/30 , G01N2001/4027 , Y10T436/2525
摘要： A method of drying a biological sample disposed on a substrate is provided. The method comprises providing the substrate comprising a sample loading area and a heat source; activating the heat source for generating heat; heating the substrate at least above 65° C.; and drying the biological sample. A device for storing sample is also provided, wherein the device comprises a substrate for biological sample-storage; and a heating component that generates heat to maintain a temperature of at least above 65° C. The heating component may contain one or more reagents, wherein the reagents generate heat to maintain a temperature of at least above 65° C.
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公开(公告)号：EP2652175A1
公开(公告)日：2013-10-23
申请号：EP11848353.6
申请日：2011-12-14
IPC分类号： C25B7/00 , G01N27/447 , C07H21/00
CPC分类号： C12N15/101
摘要： In accordance with the present disclosure, a method for extracting genetic material from a biological sample stored on a solid medium is provided. The method includes obtaining the solid medium, wherein the biological sample is applied on the solid medium, and the solid medium includes chemicals that lysed the biological sample and preserved the genetic material. The method also includes electroeluting the genetic material directly from the solid medium to a subsequent medium.
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公开(公告)号：EP3025150B1
公开(公告)日：2017-09-06
申请号：EP14750885.7
申请日：2014-07-25
发明人： PULEO, Christopher Michael , NELSON, John Richard , SPOONER, Patrick McCoy , LENIGK, Ralf , WOOD, Nichole Lea , ZHU, Li , GALLIGAN, Craig Patrick
IPC分类号： G01N27/327 , G01N27/447 , H01G11/48
CPC分类号： B01D57/02 , G01N27/4473
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公开(公告)号：EP3164511A1
公开(公告)日：2017-05-10
申请号：EP15814648.0
申请日：2015-06-26
发明人： NELSON, John Richard , DUTHIE, Robert Scott , PULEO, Christopher Michael , SPOONER, Patrick McCoy
IPC分类号： C12Q1/70
CPC分类号： C12Q1/6848 , C12Q2531/119 , C12Q2531/125 , C12Q2565/518
摘要： A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; applying an aqueous buffer to the sample application zone of the substrate to washes away one or more inhibitors present on the sample application zone; and applying an isothermal nucleic acid amplification reaction mixture to the sample application zone to amplify the target nucleic acid to form a nucleic acid amplification product. The target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight.
摘要翻译： 本文提供了一种方法，该方法包括：将包含靶核酸的样品施加至基板的样品施加区域; 将水性缓冲液施加到所述基底的所述样品施加区域以洗去所述样品施加区域上存在的一种或多种抑制剂; 以及将等温核酸扩增反应混合物应用于样品施加区以扩增靶核酸以形成核酸扩增产物。 具有第一分子量的靶核酸基本上固定在样品施加区，并且其中扩增产物具有第二分子量。
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8.发明公开DEVICES AND SYSTEMS FOR ELUTION OF BIOMOLECULES 有权VORRICHTUNGEN UND SYSTEME ZURAUFLÖSUNGVONBIOMOLEKÜLEN
公开(公告)号：EP3025150A1
公开(公告)日：2016-06-01
申请号：EP14750885.7
申请日：2014-07-25
发明人： PULEO, Christopher Michael , NELSON, John Richard , SPOONER, Patrick McCoy , LENIGK, Ralf , WOOD, Nichole Lea , ZHU, Li , GALLIGAN, Craig Patrick
IPC分类号： G01N27/327 , G01N27/447 , H01G11/48
CPC分类号： B01D57/02 , G01N27/4473
摘要： A device and a system for eluting biomolecules from biological sample by electroelution are provided. The device for electroelution of biomolecules from a biological sample is constituted with a housing configured to receive an electrolyte and the biological sample, at least two electrodes comprising conductive redox polymers operationally coupled to the housing, and a biomolecule impermeable layer disposed on at least one of the electrodes. The biomolecule impermeable layer disposed on at least one of the electrodes to prevent the biomolecules from reaching the electrode. A system is provided, wherein the system comprises a sample collection port, one or more reservoirs comprising a buffer, a solvent, a reagent or combinations thereof, an device for electroelution, and a controller.
摘要翻译： 提供了一种通过电洗脱从生物样品中洗脱生物分子的装置和系统。 用于从生物样品电洗脱生物分子的装置由构造成接收电解质和生物样品的壳体构成，至少两个电极包括可操作地耦合到壳体的导电氧化还原聚合物，以及生物分子不可渗透层，其设置在至少一个 电极。 生物分子不渗透层设置在至少一个电极上以防止生物分子到达电极。 提供了一种系统，其中系统包括样品采集端口，一个或多个包含缓冲液，溶剂，试剂或其组合的储存器，用于电洗脱的装置和控制器。
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