利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

479 条记录 (耗时 0.022 秒)

    RAPID METHOD FOR ISOLATING EXTRACELLULAR NUCLEIC ACIDS
    6.
    发明公开
    RAPID METHOD FOR ISOLATING EXTRACELLULAR NUCLEIC ACIDS 审中-公开

    公开(公告)号：EP3412773A1

    公开(公告)日：2018-12-12

    申请号：EP18186343.2

    申请日：2012-09-25

    申请人： QIAGEN GmbH

    发明人： HORLITZ, Martin ,  SPRENGER-HAUSSELS, Markus ,  GRÜNEFELD, Peter ,  NOCON, Annette ,  ERBACHER, Christoph

    IPC分类号： C12N15/10

    CPC分类号： C12Q1/6806 ,  C12N15/1006 ,  C12N15/101

    摘要： The present invention pertains to a method for isolating extracellular nucleic acids from a sample, wherein said sample is optionally stabilized, by binding the extracellular nucleic acids to a solid phase which carries anion exchange groups, comprising the following steps:
    a. binding the extracellular nucleic acids to the solid phase in a binding mixture having a first pH which allows binding the extracellular nucleic acids to the anion exchange groups of the solid phase;
    b. separating the solid phase with the bound extracellular nucleic acids;
    c. optionally washing the extracellular nucleic acids;
    d. optionally eluting extracellular nucleic acids from the solid phase.
    The method has the advantage that large sample volumes can be processed and that extracellular nucleic acids can be isolated rapidly with a high yield. The method is particularly suitable for automatable processes.