公开(公告)号：EP1616021A2

公开(公告)日：2006-01-18

申请号：EP03814988.6

申请日：2003-12-30

申请人： Gryphon Therapeutics, Inc.

发明人： KOCHENDOERFER, Gerd, G. ,  SHAO, Haiyan ,  CRESSMAN, Sonya

IPC分类号： C12Q1/00 ,  G01N33/53 ,  C07K14/00

CPC分类号： C07K1/1077 ,  C07K1/023 ,  C07K1/047 ,  C07K1/1072 ,  C07K14/535 ,  Y02P20/55

摘要： The invention concerns a multiplex polymer ligation method involving water-soluble polymeric protecting groups, compositions and methods of use. The method involves synthesis of a first compound bearing one or more water-soluble polymeric protecting groups followed by replacing one or more of the water-soluble protecting groups with a chemical adduct of interest. Multiple different compounds derived from the first compound can be readily prepared by splitting the first compound into first and second reaction systems, followed by replacing one or more of the water-soluble polymeric protecting groups of the first reaction system with a first chemical adduct, and replacing one or more of the water-soluble polymeric protecting groups of the second reaction system with a second chemical adduct of interest, where the first and second chemical adducts are different. Also provided are kits useful for carrying out the multiplex polymer ligation methods of the invention.