公开(公告)号：EP1506218B1

公开(公告)日：2017-04-12

申请号：EP03755460.7

申请日：2003-05-23

申请人： Arrowhead Pharmaceuticals, Inc.

发明人： ROZEMA, David, B. ,  WOLFF, Jon, A. ,  WAKEFIELD, Darren ,  EKENA, Kirk ,  HAGSTROM, James, E.

IPC分类号： A61K47/54 ,  C07K1/107 ,  A61P35/00

CPC分类号： C07K1/1072 ,  A61K47/60

公开(公告)号：EP2892929A4

公开(公告)日：2016-04-13

申请号：EP13834888

申请日：2013-09-05

申请人： UNIV ROCHESTER

发明人： FASAN RUDI ,  FROST JOHN R

IPC分类号： C07K19/00 ,  A61K47/48 ,  A61K49/00 ,  C07D495/04 ,  C09B57/02 ,  C12N9/24 ,  G01N33/58 ,  G01N33/68

CPC分类号： C07K1/13 ,  A61K47/48338 ,  A61K47/65 ,  A61K49/0039 ,  A61K49/0054 ,  C07C323/58 ,  C07C323/59 ,  C07C323/60 ,  C07D249/04 ,  C07D311/16 ,  C07D495/04 ,  C07K1/1072 ,  C07K14/35 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/50 ,  C07K2319/60 ,  C07K2319/92 ,  C09B57/02 ,  C12N9/2442 ,  C12Y302/01014 ,  G01N33/582 ,  G01N33/6803

摘要： Methods and compositions are provided for covalently linking a chemical species to a recombinant or synthetic polypeptide. The methods involve the reaction of a thioester-comprising polypeptide with a reagent comprising a reactive amino-thiol group connected to the chemical species which is to be covalently linked to the polypeptide, via a linker. Such chemical species can be a functional group, a label or tag molecule, a biological molecule, a ligand, or a solid support. Efficient and catalyst-free methods for C-terminal protein labeling are also provided. The methods expand current capabilities in the area of protein functionalization, providing useful and complementary tools for the isolation, detection, characterization, and analysis of proteins in a variety of in vitro and in vivo applications.

摘要翻译： 提供了用于共价连接的化学物种到一个重组或合成多肽的方法和组合物。 所述方法涉及硫酯包括用试剂包括连接到所述化学物质的所有要被共价连接到所述多肽的反应性氨基硫醇基团的多肽的反应，经由左侧。 搜索化学物质可以是官能团，标签或标记分子，生物分子，配体，或固体支持物。 因此，提供用于C-末端蛋白标记有效和无催化剂的方法。 这些方法在扩大蛋白官能化，用于分离，检测，表征和蛋白质在各种体外和体内应用的分析提供有用的和互补的工具的区域的电流的能力。

公开(公告)号：EP2410331B1

公开(公告)日：2015-09-23

申请号：EP11165160.0

申请日：2004-04-16

申请人： The Scripps Research Institute

发明人： Deiters, Alexander ,  Cropp, Ashton T. ,  Chin, Jason W. ,  Anderson, Christopher J. ,  Schultz, Peter G.

IPC分类号： C07C269/06 ,  C07C311/41

CPC分类号： G01N33/583 ,  C07C227/22 ,  C07C229/36 ,  C07C269/06 ,  C07C311/41 ,  C07K1/1072 ,  C07K1/13 ,  C07K14/001 ,  C07K16/18 ,  C09B11/08 ,  C09B57/00 ,  C12N9/93 ,  C12N15/67 ,  C12N2510/02 ,  C12P17/04 ,  C12P21/02 ,  G01N33/532 ,  G01N33/582 ,  C07C271/22

摘要： This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNAsynthetases, pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

公开(公告)号：EP2403938A4

公开(公告)日：2013-10-16

申请号：EP10749160

申请日：2010-03-01

申请人： META ACTIV INC

发明人： HUANG ALEXANDER L ,  WU GIN

IPC分类号： C12N5/04 ,  A01N65/08 ,  A61K36/185 ,  A61K36/82 ,  A61K38/44 ,  C07G1/00 ,  C07K1/107

CPC分类号： A61K36/82 ,  A01N59/00 ,  A01N65/08 ,  A23K20/111 ,  A23L33/105 ,  A61K31/05 ,  A61K31/122 ,  A61K33/40 ,  A61K36/185 ,  A61K36/22 ,  A61K38/44 ,  C07G1/00 ,  C07K1/1072 ,  A61K2300/00

摘要： Methods of and compositions for producing and using plant-based materials are provided. The methods include using biopolymers or their synthetic equivalents combined with a stable source of reactive oxygen species that when applied to or combined with a separate source of oxido-reducing enzyme or catalyst will cause the formation of an activated biopolymer with increased protein binding affinity and microbial control activities.

公开(公告)号：EP2348037A4

公开(公告)日：2013-01-16

申请号：EP09819275

申请日：2009-10-09

申请人： RIKEN

发明人： HASEGAWA KOKI ,  WATANABE YASUYOSHI

IPC分类号： A61K49/00 ,  A61K51/00 ,  A61K51/10 ,  C07K1/107 ,  C07K1/13 ,  C07K14/645 ,  C07K14/655 ,  C07K16/00

CPC分类号： C07K1/13 ,  A61K49/0002 ,  A61K51/10 ,  C07K1/1072 ,  C07K14/605 ,  C07K14/645 ,  C07K14/655 ,  Y02P20/55

公开(公告)号：EP2476691A1

公开(公告)日：2012-07-18

申请号：EP10813843.9

申请日：2010-09-07

申请人： Nissan Chemical Industries, Ltd.

发明人： KAKIUCHI, Nobuyuki ,  SHOJI, Takeaki ,  HIRASADA, Kazuki ,  MATSUMOTO, Keigo ,  YAMAGUCHI, Hiroki

IPC分类号： C07K5/062 ,  C07K1/02

CPC分类号： A61K47/48046 ,  A61K38/05 ,  A61K47/543 ,  C07K1/026 ,  C07K1/107 ,  C07K1/1072 ,  C07K1/1075 ,  C07K5/06 ,  C07K5/06026 ,  C07K5/06043 ,  C07K5/06052

摘要： There is provided a practical method for preparing lipopeptide compounds, which method is capable of inexpensive mass production without requiring complicated operations. The lipopeptide compound of formula (3):

is produced by reacting an ester compound of formula (1):

with an α-amino acid compound of formula (2):

in the presence of a base and within a solvent containing a nonpolar organic solvent.

摘要翻译： 提供了制备脂肽化合物的实用方法，该方法能够在不需要复杂操作的情况下廉价地批量生产。 式（3）的脂肽化合物：通过使式（1）的酯化合物与式（2）的±氨基酸化合物在碱的存在下和含有非极性有机溶剂的溶剂 。

公开(公告)号：EP2392923A1

公开(公告)日：2011-12-07

申请号：EP11165139.4

申请日：2004-04-16

申请人： The Scripps Research Institute

发明人： Deiters, Alexander ,  Cropp, Ashton T. ,  Chin, Jason W. ,  Anderson, Christopher J. ,  Schultz, Peter G.

IPC分类号： G01N33/53 ,  A61K38/17 ,  C12N5/00 ,  C12N9/00 ,  C12N15/85

CPC分类号： G01N33/583 ,  C07C227/22 ,  C07C229/36 ,  C07C269/06 ,  C07C311/41 ,  C07K1/1072 ,  C07K1/13 ,  C07K14/001 ,  C07K16/18 ,  C09B11/08 ,  C09B57/00 ,  C12N9/93 ,  C12N15/67 ,  C12N2510/02 ,  C12P17/04 ,  C12P21/02 ,  G01N33/532 ,  G01N33/582 ,  C07C271/22

摘要： This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNAsynthetases, pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

摘要翻译： 本发明提供了用于产生扩增真核细胞中遗传编码氨基酸数量的翻译组分的组合物和方法。 组分包括正交tRNA，正交氨酰-tRNA合成酶，tRNA /合成酶和非天然氨基酸对。 还提供了在真核细胞中产生具有非天然氨基酸的蛋白质的蛋白质和方法。

公开(公告)号：EP2378288A1

公开(公告)日：2011-10-19

申请号：EP11163742.7

申请日：2004-04-16

申请人： The Scripps Research Institute

发明人： Deiters, Alexander ,  Cropp, Ashton, T. ,  Chin, Jason, W. ,  Anderson, Christopher, J. ,  Schultz, Peter, G.

IPC分类号： G01N33/53 ,  A61K38/17 ,  C12N5/00 ,  C12N9/00 ,  C12N15/85 ,  C12N15/67

CPC分类号： G01N33/583 ,  C07C227/22 ,  C07C229/36 ,  C07C269/06 ,  C07C311/41 ,  C07K1/1072 ,  C07K1/13 ,  C07K14/001 ,  C07K16/18 ,  C09B11/08 ,  C09B57/00 ,  C12N9/93 ,  C12N15/67 ,  C12N2510/02 ,  C12P17/04 ,  C12P21/02 ,  G01N33/532 ,  G01N33/582 ,  C07C271/22

摘要： This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNAsynthetases, pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

公开(公告)号：EP1506218A4

公开(公告)日：2010-08-11

申请号：EP03755460

申请日：2003-05-23

申请人： MIRUS BIO CORP

发明人： ROZEMA DAVID B ,  WOLFF JON A ,  WAKEFIELD DARREN ,  EKENA KIRK ,  HAGSTROM JAMES E

IPC分类号： C07D307/60 ,  A61K38/00 ,  A61K45/00 ,  A61K47/48 ,  A61K48/00 ,  C07K7/06 ,  A61P35/00 ,  C07K1/107

CPC分类号： C07K1/1072 ,  A61K47/60

公开(公告)号：EP1978989A2

公开(公告)日：2008-10-15

申请号：EP06849074.7

申请日：2006-12-27

申请人： Ambrx, Inc.

发明人： MIAO, Zhenwei ,  LIU, Junjie

IPC分类号： A61K38/16

CPC分类号： C07K1/1072 ,  C07D231/12 ,  C07K14/61 ,  C12N9/93

摘要： Disclosed herein are non-natural amino acids and polypeptides that include at least one non-natural amino acid, and methods for making such non-natural amino acids and polypeptides. The non-natural amino acids, by themselves or as a part of a polypeptide, can include a wide range of possible functionalities, but typical have at least one heterocycle, aldol-based, dicarbonyl, and/or diamine group. Also disclosed herein are non-natural amino acid polypeptides that are further modified post-translationally, methods for effecting such modifications, and methods for purifying such polypeptides. Typically, the modified non-natural amino acid polypeptides include at least one heterocycle, aldol-based, dicarbonyl, and/or diamine group. Further disclosed are methods for using such non-natural amino acid polypeptides and modified non-natural amino acid polypeptides, including therapeutic, diagnostic, and other biotechnology uses.