摘要:
It is intended to provide a technique of producing a sterile plant by inhibiting the transcription of a gene participating in flower organ formation. A chimeric gene composed of a gene, which encodes a transcriptional factor promoting the expression of a gene participating in flower organ formation, with a polynucleotide, which encodes a functional peptide converting an arbitrary transcriptional factor into a transcriptional inhibitor, is transferred into a plant cell to thereby produce a chimeric protein composed of the above-described transcriptional factor and the above-described functional peptide fused together. As the results of the predominant inhibition of the expression of the gene participating in flower organ formation by the chimeric protein, a male sterile plant which is unable to form normal pollen is produced. The chimeric protein also inhibits the expression of a gene participating in anther dehiscence to thereby give a plant with inhibited anther dehiscence. Furthermore, the chimeric protein inhibits the expression of a gene targeted by a transcriptional factor participating in the formation of stamen and pistil to thereby give a double-flowered plant.
摘要:
This invention is intended to be used to search for a transcription factor having novel functions of increasing the weight of an individual plant, increasing the weight of a given tissue per individual plant, or improving the productivity of a given substance per individual plant and to improve such properties in the plant. The weight of an individual plant is increased, the weight of a given tissue per individual plant is increased, the productivity of a given substance per individual plant is improved, or the content of a given substance per given tissue of a plant is increased via expression of a transcription factor that has been modified to suppress transcription accelerating activity.
摘要:
This invention is intended to be used to search for a transcription factor having novel functions of increasing the weight of an individual plant, increasing the weight of a given tissue per individual plant, or improving the productivity of a given substance per individual plant and to improve such properties in the plant. The weight of an individual plant is increased, the weight of a given tissue per individual plant is increased, the productivity of a given substance per individual plant is improved, or the content of a given substance per given tissue of a plant is increased via expression of a transcription factor that has been modified to suppress transcription accelerating activity.
摘要:
A peptide or a protein capable of converting a transcriptional factor into a transcription regulator; a gene encoding the above peptide or protein; a chimeric protein wherein the above peptide or protein is bonded to a transcriptional factor; a chimeric gene wherein a gene encoding the above peptide or protein is bonded to another gene encoding a transcriptional factor; a recombinant vector having the chimeric gene; and a transformant containing the recombinant vector. Because of having an extremely short length, the above-described peptide capable of converting a transcriptional factor into a transcription regulator can be very easily synthesized and makes it possible to efficiently regulate the transcription targeting a specific gene alone. Owing to these characteristics, this peptide is widely applicable and useful in, for example, regulating the expression of an oncogene and regulating the expression of a gene encoding an enzyme in a pigment metabolism system.