摘要:
DNA sequences capable of encoding an enzyme protein functioning as a rice dihydrodipicolinate synthase (DHDPS). An example of the above DNA sequences is one having the base sequence represented by SEQ ID NO:1 in the Sequence Listing. Moreover, a DNA having the base sequence represented by SEQ ID NO:5 in the Sequence Listing and one having the base sequence represented by SEQ ID NO:7 each capable of encoding the protein with a DHDPS activity are obtained. By culturing vegetable cells having, transferred thereinto, a vector which carries a DNA fragment having such a DNA integrated into the downstream of a promoter, a transformed plant with a high lysine content can be regenerated.
摘要:
A DNA sequence capable of encoding an enzyme protein which is the α-subunit of the first isozyme of rice anthranilate synthase (ASA); and a DNA capable of encoding an enzyme protein which is the α-subunit of the second isozyme of ASA. A transformant plant with a high tryptophan content can be regenerated by culturing vegetable cells into which a recombinant vector constructed by integrating a DNA fragment carrying the above DNA sequence into the downstream of a promoter has been transferred. The invention also provides a DNA having a promoter activity on the rice ASA gene, which is usable as a promoter for transferring a useful foreign gene into rice or other plants and then expressing the same.