公开(公告)号：EP1026249B1

公开(公告)日：2008-12-10

申请号：EP98940641.8

申请日：1998-08-31

申请人： HOKKO CHEMICAL INDUSTRY CO. LTD. ,  Incorporated Administrative Agency National Agriculture and Bio-oriented Research Organization

发明人： WAKASA, Kyo ,  TOZAWA, Yuzuru ,  TERAKAWA, Teruhiko ,  HASEGAWA, Hisakazu

IPC分类号： C12N15/60 ,  A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C12N1/21 ,  C12N15/82 ,  C12N9/88

CPC分类号： C12N15/8254 ,  C12N9/88 ,  C12N15/8207 ,  C12N15/8209 ,  C12N15/8216 ,  C12N15/8251 ,  C12Y401/03027

摘要： A DNA sequence capable of encoding an enzyme protein which is the α-subunit of the first isozyme of rice anthranilate synthase (ASA); and a DNA capable of encoding an enzyme protein which is the α-subunit of the second isozyme of ASA. A transformant plant with a high tryptophan content can be regenerated by culturing vegetable cells into which a recombinant vector constructed by integrating a DNA fragment carrying the above DNA sequence into the downstream of a promoter has been transferred. The invention also provides a DNA having a promoter activity on the rice ASA gene, which is usable as a promoter for transferring a useful foreign gene into rice or other plants and then expressing the same.

公开(公告)号：EP1026249A1

公开(公告)日：2000-08-09

申请号：EP98940641.8

申请日：1998-08-31

申请人： HOKKO CHEMICAL INDUSTRY CO. LTD. ,  Japan as repres. by Director General of National Agriculture Research Center, Ministry of Agriculture, Forestry and Fisheries

发明人： WAKASA, Kyo ,  TOZAWA, Yuzuru ,  TERAKAWA, Teruhiko ,  HASEGAWA, Hisakazu

IPC分类号： C12N15/60 ,  A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C12N1/21

CPC分类号： C12N15/8254 ,  C12N9/88 ,  C12N15/8207 ,  C12N15/8209 ,  C12N15/8216 ,  C12N15/8251 ,  C12Y401/03027

摘要： This invention provides a DNA sequence capable of encoding an enzyme protein which is the α-subunit of the first isozyme of rice anthranilate synthase (ASA); and a DNA capable of encoding an enzyme protein which is the α-subunit of the second isozyme of ASA. Transformed plants having a high tryptophan content can be regenerated by culturing the plant cells having introduced therein such a recombinant vector which contains, at a position downstream of a promoter, an inserted DNA fragment carrying the above DNA sequence.
This invention also provides a DNA having a promoter activity for the rice ASA gene, which DNA can be used as a promoter for introducing a useful exogenous gene into rice or other plants and expressing the gene therein.

摘要翻译： 本发明提供了能够编码蛋白质的所有这是稻邻氨基苯甲酸合酶的同功酶第一（ASA）的α亚单位的酶的DNA序列; 和能够编码蛋白质的所有这是ASA的第二同工酶的α亚单位的酶的DNA。 装载上述DNA序列插入的DNA片段的具有高色氨酸含量的转化植物可以通过培养导入了的植物细胞。其中寻求其中含有的重组载体，在启动子的下游的位置处被再生。 本发明因此提供了一种具有用于稻ASA基因，该基因的DNA可被用作用于将有用的外源基因导入水稻或其他植物及所表达该基因的启动子的启动子活性的DNA。