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公开(公告)号：EP3322808A1
公开(公告)日：2018-05-23
申请号：EP16747632.4
申请日：2016-07-15
发明人： SHINKAWA, Satoru , MITSUZAWA, Shigenobu , TANAKA, Maiko , MACHIDA, Masayuki , HAGIWARA, Hiroko , KOIKE, Hideaki , KUNINAGA, Mahoko
CPC分类号： C12N15/80 , C07K14/38 , C12N9/2402 , C12N15/63 , C12Y302/01031
摘要： To provide a base sequence for protein expression that can increase the yield of protein such as diastatic enzyme by further activating a promoter of a particular gene. A base sequence 1 for protein expression includes: a gene 3 encoding protein 2; a promoter 4 of the gene 3, the promoter being linked upstream of the gene 3; and a cis element 5 whose activity is improved by an artificial transcription factor 6, the cis element being linked further upstream of the promoter 4. The cis element 5 is represented by SEQ ID NO: 1.
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公开(公告)号：EP3322808B1
公开(公告)日：2018-12-26
申请号：EP16747632.4
申请日：2016-07-15
发明人： SHINKAWA, Satoru , MITSUZAWA, Shigenobu , TANAKA, Maiko , MACHIDA, Masayuki , HAGIWARA, Hiroko , KOIKE, Hideaki , KUNINAGA, Mahoko
CPC分类号： C12N15/80 , C07K14/38 , C12N9/2402 , C12N15/63 , C12Y302/01031
摘要： To provide a base sequence for protein expression that can increase the yield of protein such as diastatic enzyme by further activating a promoter of a particular gene. A base sequence 1 for protein expression includes: a gene 3 encoding protein 2; a promoter 4 of the gene 3, the promoter being linked upstream of the gene 3; and a cis element 5 whose activity is improved by an artificial transcription factor 6, the cis element being linked further upstream of the promoter 4. The cis element 5 is represented by SEQ ID NO: 1.
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公开(公告)号：EP3322806A1
公开(公告)日：2018-05-23
申请号：EP16745514.6
申请日：2016-07-15
发明人： SHINKAWA, Satoru , MITSUZAWA, Shigenobu , TANAKA, Maiko , MACHIDA, Masayuki , HAGIWARA, Hiroko , KOIKE, Hideaki , KUNINAGA, Mahoko
摘要： To provide a base sequence for protein expression that can increase the yield of protein such as diastatic enzyme by further activating a promoter of a particular gene. A base sequence 1 for protein expression includes: a gene 3 encoding protein 2; a promoter 4 of the gene 3, the promoter being linked upstream of the gene 3; and a cis element 5 whose activity is improved by an artificial transcription factor 6, the cis element being linked further upstream of the promoter 4. The cis element 5 is represented by SEQ ID NO: 1.
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公开(公告)号：EP3322807B1
公开(公告)日：2018-12-05
申请号：EP16747631.6
申请日：2016-07-15
发明人： SHINKAWA, Satoru , MITSUZAWA, Shigenobu , TANAKA, Maiko , MACHIDA, Masayuki , HAGIWARA, Hiroko , KOIKE, Hideaki , KUNINAGA, Mahoko
CPC分类号： C12N15/80 , C12N1/14 , C12N15/113 , C12N15/62 , C12N15/63
摘要： To provide a base sequence for protein expression that can increase the yield of protein such as diastatic enzyme by further activating a promoter of a particular gene. A base sequence 1 for protein expression includes: a gene 3 encoding protein 2; a promoter 4 of the gene 3, the promoter being linked upstream of the gene 3; and a cis element 5 whose activity is improved by an artificial transcription factor 6, the cis element being linked further upstream of the promoter 4. The cis element 5 is represented by SEQ ID NO: 1.
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公开(公告)号：EP3322806B1
公开(公告)日：2018-10-17
申请号：EP16745514.6
申请日：2016-07-15
发明人： SHINKAWA, Satoru , MITSUZAWA, Shigenobu , TANAKA, Maiko , MACHIDA, Masayuki , HAGIWARA, Hiroko , KOIKE, Hideaki , KUNINAGA, Mahoko
摘要： To provide a base sequence for protein expression that can increase the yield of protein such as diastatic enzyme by further activating a promoter of a particular gene. A base sequence 1 for protein expression includes: a gene 3 encoding protein 2; a promoter 4 of the gene 3, the promoter being linked upstream of the gene 3; and a cis element 5 whose activity is improved by an artificial transcription factor 6, the cis element being linked further upstream of the promoter 4. The cis element 5 is represented by SEQ ID NO: 1.
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公开(公告)号：EP3322807A1
公开(公告)日：2018-05-23
申请号：EP16747631.6
申请日：2016-07-15
发明人： SHINKAWA, Satoru , MITSUZAWA, Shigenobu , TANAKA, Maiko , MACHIDA, Masayuki , HAGIWARA, Hiroko , KOIKE, Hideaki , KUNINAGA, Mahoko
CPC分类号： C12N15/80 , C12N1/14 , C12N15/113 , C12N15/62 , C12N15/63
摘要： To provide a base sequence for protein expression that can increase the yield of protein such as diastatic enzyme by further activating a promoter of a particular gene. A base sequence 1 for protein expression includes: a gene 3 encoding protein 2; a promoter 4 of the gene 3, the promoter being linked upstream of the gene 3; and a cis element 5 whose activity is improved by an artificial transcription factor 6, the cis element being linked further upstream of the promoter 4. The cis element 5 is represented by SEQ ID NO: 1.
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公开(公告)号：EP3054004B1
公开(公告)日：2017-12-27
申请号：EP16153909.3
申请日：2016-02-02
CPC分类号： C12N1/14 , C07K14/38 , C12N9/24 , C12N9/2437 , C12N9/485 , C12N9/62 , C12Y302/01091
摘要： Provided are an Aspergillus mutant strain which can dramatically enhance production of a heterologous enzyme when a saccharifying enzyme gene is transferred into the strain to perform transformation and a transformant having a saccharifying enzyme gene transferred into the Aspergillus mutant strain. The Aspergillus mutant strain has been completely or partially deficient in three genes of Aspergillus oryzae strain HO2 (accession number: NITE BP-01750): a prtR gene coding for a transcription factor; a pepA gene coding for an extracellular acid protease; and a cpI gene coding for an extracellular acid carboxypeptidase.
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公开(公告)号：EP2826859B1
公开(公告)日：2017-05-31
申请号：EP14175790.6
申请日：2014-07-04
发明人： TANAKA, Maiko , MITSUZAWA, Shigenobu , SHINKAWA, Satoru , SHIBATA, Daisuke , ARA, Takeshi , TAKEDA, Migiwa
IPC分类号： C12N9/42
CPC分类号： C12N9/2445 , C12P19/02 , C12P19/14 , C12Y302/01021
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公开(公告)号：EP3054004A1
公开(公告)日：2016-08-10
申请号：EP16153909.3
申请日：2016-02-02
CPC分类号： C12N1/14 , C07K14/38 , C12N9/24 , C12N9/2437 , C12N9/485 , C12N9/62 , C12Y302/01091
摘要： Provided are an Aspergillus mutant strain which can dramatically enhance production of a heterologous enzyme when a saccharifying enzyme gene is transferred into the strain to perform transformation and a transformant having a saccharifying enzyme gene transferred into the Aspergillus mutant strain. The Aspergillus mutant strain has been completely or partially deficient in three genes of Aspergillus oryzae strain HO2 (accession number: NITE BP-01750): a prtR gene coding for a transcription factor; a pepA gene coding for an extracellular acid protease; and a cpI gene coding for an extracellular acid carboxypeptidase.
摘要翻译： 提供了当将糖化酶基因转移到菌株进行转化时，可以显着提高异源酶的产生的曲霉菌突变菌株和将糖化酶基因转移到曲霉菌突变株中的转化体。 曲霉菌突变菌株在米曲霉菌株HO2（登录号：NITE BP-01750）的三个基因中完全或部分缺陷：编码转录因子的prtR基因; 编码细胞外酸性蛋白酶的pepA基因; 和编码细胞外酸羧肽酶的cpI基因。
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