公开(公告)号：EP0959141A2

公开(公告)日：1999-11-24

申请号：EP99109795.7

申请日：1999-05-18

申请人： Hitachi, Ltd.

发明人： Muramatsu, Takamichi ,  Fujita, Takeshi ,  Kiyama, Masaharu ,  Irie, Takashi ,  Okano, Kazunori

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  C12Q1/6834

摘要： Focusing that abundant class genes abundantly present in a nucleic acid sample can comparatively easily be analyzed and readily be removed in a selective manner, a nucleic acid sample for expression analysis of rare expressed genes can be obtained by removing abundant genes therefrom, and analyses based upon the sample can be achieved.

摘要翻译： 聚焦并丰富类基因中大量存在的核酸样品中可以比较容易地进行分析，并容易地以选择性的方式被去除，为的稀有过表达的基因的表达分析的核酸样品可以通过从除去丰富基因那里获得，并且分析基于 样品就可以实现。

公开(公告)号：EP0803730A2

公开(公告)日：1997-10-29

申请号：EP97106804.4

申请日：1997-04-24

申请人： HITACHI, LTD. ,  HITACHI ELECTRONICS ENGINEERING CO., LTD.

发明人： Fujita, Takeshi, Hitachi-Hatoyama-Ryo 103 ,  Kiyama, Masaharu ,  Umemura, Shin-ichiro ,  Muramatsu, Takamichi ,  Miyazaki, Yuusuke, Nisshin-Haitsu-Nagahama 202

IPC分类号： G01N27/447

CPC分类号： G01N27/44704 ,  G01N21/64 ,  G01N27/44708 ,  G01N27/44721 ,  G01N27/44734 ,  G01N2021/0389 ,  G01N2021/151

摘要： Arranging a pair of temperature control units (29, 131, 132, 50) on both sides of gel 1 and arranging light-transmitting slit 134 on one of the elements, irradiation of an excitation beam over the gel 1 and signal detection are practiced through the slit 134. Introducing dry air onto the slit part, mildew occurrence is prevented on the detecting part. Detecting the power level applied to the gel and calculating the temperature of the temperature control units so that the gel temperature might be a predetermined temperature, on the basis of the detected power level, thereafter carrying out the feedback control of the power level, the gel temperature can be controlled appropriately and strictly during electrophoresis in an automatic fluorescent electrophoresis system, so that high-speed analysis can be done highly reproducibly at a higher voltage applied even by SSCP.

公开(公告)号：EP0803730A3

公开(公告)日：1999-07-07

申请号：EP97106804.4

申请日：1997-04-24

申请人： HITACHI, LTD. ,  HITACHI ELECTRONICS ENGINEERING CO., LTD.

发明人： Fujita, Takeshi, Hitachi-Hatoyama-Ryo 103 ,  Kiyama, Masaharu ,  Umemura, Shin-ichiro ,  Muramatsu, Takamichi ,  Miyazaki, Yuusuke, Nisshin-Haitsu-Nagahama 202

IPC分类号： G01N27/447

CPC分类号： G01N27/44704 ,  G01N21/64 ,  G01N27/44708 ,  G01N27/44721 ,  G01N27/44734 ,  G01N2021/0389 ,  G01N2021/151

摘要： Arranging a pair of temperature control units (29, 131, 132, 50) on both sides of gel 1 and arranging light-transmitting slit 134 on one of the elements, irradiation of an excitation beam over the gel 1 and signal detection are practiced through the slit 134. Introducing dry air onto the slit part, mildew occurrence is prevented on the detecting part. Detecting the power level applied to the gel and calculating the temperature of the temperature control units so that the gel temperature might be a predetermined temperature, on the basis of the detected power level, thereafter carrying out the feedback control of the power level, the gel temperature can be controlled appropriately and strictly during electrophoresis in an automatic fluorescent electrophoresis system, so that high-speed analysis can be done highly reproducibly at a higher voltage applied even by SSCP.

摘要翻译： 在凝胶1两侧安排一对温度控制单元（29,131,132,50），并且在其中一个元件上布置透光狭缝134，通过凝胶1上的激发光束的照射和信号检测通过 狭缝134.在狭缝部分上引入干燥空气，防止了检测部件发生霉变。 检测施加到凝胶上的功率水平并计算温度控制单元的温度，使得基于检测到的功率水平，凝胶温度可以是预定温度，然后进行功率水平的反馈控制，凝胶 在自动荧光电泳系统的电泳过程中，可以适当且严格地控制温度，从而即使通过SSCP施加的高电压也能高度重复地进行高速分析。