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1.发明公开METHOD FOR ANALYZING BIOMOLECULES AND BIOMOLECULE ANALYZER 审中-公开VERFAHREN ZUR ANALYZE VONBIOMOLEKÜLENUNDBIOMOLEKÜLANALYSATOR
公开(公告)号：EP2765424A1
公开(公告)日：2014-08-13
申请号：EP12838806.3
申请日：2012-10-04
发明人： SAITO, Toshiro , HAMASAKI, Koshin , TAKAHASHI, Satoshi , MAESHIMA, Muneo , IMAI, Kyoko , IMAI, Kazumichi , TAO, Ryuji
IPC分类号： G01N33/553 , C12M1/00 , C12Q1/68 , G01N21/64 , G01N33/543
CPC分类号： C12Q1/6834 , C12Q1/6804 , C12Q1/6837 , G01N21/6428 , G01N21/6452 , G01N33/54326 , G01N33/54346 , G01N33/54393 , G01N33/587 , G01N2021/6421 , C12Q2525/161 , C12Q2545/114 , C12Q2563/107 , C12Q2563/143 , C12Q2563/149 , C12Q2565/514 , C12Q2563/119
摘要： An object of the present invention is to provide a method and means for analyzing biomolecules that can realize, in biomolecule analysis, a wide dynamic range attained by counting the number of biomolecules and rapid analysis. The present invention relates to a method for analyzing biomolecules, comprising the steps of: immobilizing biomolecules 101 to be analyzed on surfaces of magnetic microparticles 108; reacting labeled probe molecules 104 with the biomolecules 101 to be analyzed; collecting and immobilizing the microparticles 108 on a support substrate 110; and measuring a label on the support substrate 110. Since single-molecule immobilized magnetic microparticles are used in the present invention, the number of biomolecules can be counted, and since hybridization and an antigen-antibody reaction are performed with the microparticles having biomolecules immobilized thereon dispersed, the reaction can be rapidly performed. Further, the type and the abundance of biomolecules of interest can be determined at a single molecular level, so as to evaluate, in particular, the absolute concentration of biomolecules.
摘要翻译： 本发明的目的是提供一种用于分析生物分子的方法和装置，其可以在生物分子分析中实现通过计数生物分子数量和快速分析获得的宽动态范围。 本发明涉及一种分析生物分子的方法，其特征在于包括以下步骤：将待分析的生物分子101固定在磁性微粒108的表面上; 使标记的探针分子104与待分析的生物分子101反应; 将微粒108收集并固定在支撑基板110上; 并测量支撑基板110上的标签。由于在本发明中使用单分子固定的磁性微粒，因此可以计数生物分子的数量，并且由于在其上固定有生物分子的微粒进行杂交和抗原 - 抗体反应 分散，可迅速进行反应。 此外，感兴趣的生物分子的类型和丰度可以在单个分子水平上确定，以便特别地评估生物分子的绝对浓度。
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公开(公告)号：EP2871460A1
公开(公告)日：2015-05-13
申请号：EP13813834.2
申请日：2013-06-21
CPC分类号： C12N15/1013 , C12Q1/6806 , C12Q2527/125 , C12Q2547/10 , C12Q2563/143
摘要： A nucleic acid extractor reducing the possibility of cross contamination and a gene analysis apparatus having a nucleic acid amplification function and a detection function are provided. The nucleic acid extractor has a kit for nucleic acid extraction using silica-coated magnetic beads under the presence of a chaotropic agent, and includes a magnet cover 52 accommodating a magnet 44 in the inside and separating the magnet 44 and a reaction container 2, a wall part 53 covering the outside of the reaction container 2 in a state of accommodating at least a portion of the magnet cover 52 in the reaction container, and a wall part 53 covering a space above the reaction container 2 in a state of accommodating at least a portion of the magnet cover 52 in the reaction container. Scattering of liquid and aerosol can be prevented and the possibility of cross contamination can be reduced.
摘要翻译： 提供降低交叉污染可能性的核酸提取器和具有核酸扩增功能和检测功能的基因分析装置。 核酸提取器具有在离液剂存在下使用二氧化硅包覆的磁珠进行核酸提取的试剂盒，并且包括在内部容纳磁体44并分离磁体44和反应容器2的磁体盖52， 在反应容器2的至少一部分收容磁铁罩52的状态下覆盖反应容器2的外部的壁部53，以及至少收容在反应容器2的上方的空间的壁部53 反应容器中的磁体盖52的一部分。 可以防止液体和气溶胶的散射，并减少交叉污染的可能性。
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公开(公告)号：EP2765424B1
公开(公告)日：2018-12-05
申请号：EP12838806.3
申请日：2012-10-04
发明人： SAITO, Toshiro , HAMASAKI, Koshin , TAKAHASHI, Satoshi , MAESHIMA, Muneo , IMAI, Kyoko , IMAI, Kazumichi , TAO, Ryuji
IPC分类号： G01N33/553 , C12M1/00 , C12Q1/68 , G01N21/64 , G01N33/543
CPC分类号： C12Q1/6834 , C12Q1/6804 , C12Q1/6837 , G01N21/6428 , G01N21/6452 , G01N33/54326 , G01N33/54346 , G01N33/54393 , G01N33/587 , G01N2021/6421 , C12Q2525/161 , C12Q2545/114 , C12Q2563/107 , C12Q2563/143 , C12Q2563/149 , C12Q2565/514 , C12Q2563/119
摘要： An object of the present invention is to provide a method and means for analyzing biomolecules that can realize, in biomolecule analysis, a wide dynamic range attained by counting the number of biomolecules and rapid analysis. The present invention relates to a method for analyzing biomolecules, comprising the steps of: immobilizing biomolecules 101 to be analyzed on surfaces of magnetic microparticles 108; reacting labeled probe molecules 104 with the biomolecules 101 to be analyzed; collecting and immobilizing the microparticles 108 on a support substrate 110; and measuring a label on the support substrate 110. Since single-molecule immobilized magnetic microparticles are used in the present invention, the number of biomolecules can be counted, and since hybridization and an antigen-antibody reaction are performed with the microparticles having biomolecules immobilized thereon dispersed, the reaction can be rapidly performed. Further, the type and the abundance of biomolecules of interest can be determined at a single molecular level, so as to evaluate, in particular, the absolute concentration of biomolecules.
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公开(公告)号：EP2735618B1
公开(公告)日：2017-12-06
申请号：EP12815243.6
申请日：2012-05-16
发明人： SAITO, Toshiro , HAMASAKI, Koshin , TAKAHASHI, Satoshi , MAESHIMA, Muneo , IMAI, Kyoko , IMAI, Kazumichi , TAO, Ryuji
CPC分类号： C12Q1/6834 , C12N15/1065 , C12Q2525/207 , C12Q2537/125 , C12Q2537/143 , C12Q2563/143 , C12Q2563/149
摘要： The object of the present invention is to provide a convenient method for nucleic acid analysis, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provide is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. The present invention enables nucleic acids to be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.
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公开(公告)号：EP3121262A1
公开(公告)日：2017-01-25
申请号：EP14886521.5
申请日：2014-09-19
发明人： UEMATSU, Chihiro , MAESHIMA, Muneo , MASUYA, Akira
摘要： Provided is a test apparatus in which a test for bacterial identification or antimicrobial susceptibility can be promptly determined. A division state of bacteria is monitored by performing microscopic observation of shapes and the number of the bacteria in each of wells in a culture plate for bacterial identification culture or an antimicrobial susceptibility test, and it is determined whether or not the bacteria grow in a stage shifted from an induction phase to a logarithmic phase, with reference to an image obtained through microscopic observation. In addition, determination performed based on turbidity in the related art may be combined with determination performed based on microscopic observation in which change and the like in the shapes of the bacteria are monitored. Accordingly, it is possible to realize a highly accurate test result.
摘要翻译： 提供了可以迅速确定细菌鉴定或抗微生物药敏试验的试验装置。 通过对用于细菌鉴定培养的培养板或抗微生物敏感性试验中的每个孔中的细孔的形状和细菌的数量进行微观观察来监测细菌的分裂状态，并且确定细菌是否在一个阶段中生长 参考通过显微镜观察获得的图像，从诱导相位移位到对数阶段。 此外，基于相关技术中的浊度进行的测定可以与基于显微观察进行的确定相组合，其中监测细菌形状的变化等。 因此，可以实现高精度的测试结果。
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公开(公告)号：EP2871460B1
公开(公告)日：2016-09-21
申请号：EP13813834.2
申请日：2013-06-21
CPC分类号： C12N15/1013 , C12Q1/6806 , C12Q2527/125 , C12Q2547/10 , C12Q2563/143
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公开(公告)号：EP3330698A1
公开(公告)日：2018-06-06
申请号：EP16830326.1
申请日：2016-07-13
发明人： NOBUKI, Shunichiro , MAESHIMA, Muneo , IMAI, Kenta
IPC分类号： G01N21/76
摘要： Provided is a detection device for luminescence analysis that has a high S/N ratio and high photodetection efficiency and wherein optical filter damage and deviation of the central axis of a highly reflective light-guide system or optical filter in relation to a photodetector or window material can be prevented. A highly reflective light-guide system 201 has a highly reflective light-guide surface for reflecting light that has been emitted from a sample and has entered from an entry port opposing a window material 104 and propagating the same to an exit port opposing a light reception surface of a photodetector 106. An optical filter 203 is provided in a space surrounded by the window material 104, the photodetector 106, and the highly reflective light-guide system 201 and transmits the signal luminescence to be measured that is emitted from the sample between the window material 104 and photodetector 106. The optical filter 203 is fixed to the window material 104 or photodetector 106 by an adhesive 401, and the peripheral shape of the optical filter 203 is smaller than the shape of the inside of a fitting part to which the optical filter 203 is fitted and that is formed on the highly reflective light-guide system 201.
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公开(公告)号：EP3252138A1
公开(公告)日：2017-12-06
申请号：EP16743230.1
申请日：2016-01-22
发明人： UEMATSU, Chihiro , MAESHIMA, Muneo
CPC分类号： C12M41/36 , C12M1/34 , C12M23/12 , C12M41/46 , C12Q1/04 , C12Q1/08 , G02B21/26 , G02B21/367 , G02B21/368 , G06T1/00 , G06T7/0016 , G06T2207/10056
摘要： The invention provides a technology for promptly determining bacterial identification or an antimicrobial susceptibility testing. In the invention, first, a state where the bacteria are divided is monitored by performing microscopic observation with respect to the shape or the number of bacteria in each of wells of a culture plate for bacterial identification culture or the antimicrobial susceptibility testing. In addition, the shape, the number or the area of the bacteria are interpreted from the image obtained by the microscopic observation whether or not the bacteria proliferate at a stage from an induction phase to a logarithmic phase, and the time-dependent changes thereof are made into a graph. From the graph, it is determined whether or not the bacteria proliferate for each measurement, the determination results are displayed on the screen, and accordingly, the result of the antimicrobial susceptibility is provided every time when the measurement is performed ( Fig. 12 ).
摘要翻译： 本发明提供了用于快速确定细菌鉴定或抗微生物药物敏感性测试的技术。 在本发明中，首先，通过对用于细菌鉴定培养或抗微生物敏感性测试的培养板的每个孔中的细菌的形状或细菌数目进行显微镜观察来监测细菌分裂的状态。 另外，根据由显微镜观察获得的图像来解释细菌的形状，数量或面积，细菌是否在从诱导期到对数期的阶段增殖，并且其时间依赖性变化是 做成一张图。 从图中可以确定每次测量细菌是否增殖，测定结果显示在屏幕上，因此，每次进行测量时都提供抗菌敏感性结果（图12）。
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9.发明公开NUCLEIC ACID ANALYSIS METHOD AND ANALYSIS APPARATUS 有权NUKLEINSÄURE-ANALYSEVERFAHREN UND-ANALYSEVORRICHTUNG
公开(公告)号：EP2735618A1
公开(公告)日：2014-05-28
申请号：EP12815243.6
申请日：2012-05-16
发明人： SAITO, Toshiro , HAMASAKI, Koshin , TAKAHASHI, Satoshi , MAESHIMA, Muneo , IMAI, Kyoko , IMAI, Kazumichi , TAO, Ryuji
CPC分类号： C12Q1/6834 , C12N15/1065 , C12Q2525/207 , C12Q2537/125 , C12Q2537/143 , C12Q2563/143 , C12Q2563/149
摘要： The object of the present invention is to provide a convenient method for nucleic acid analysis, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provide is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower.
The present invention enables nucleic acids to be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.摘要翻译： 本发明的目的是提供一种方便的核酸分析方法，其使得能够以高全面性和至少四位数的动态范围共同分析1000种或更多种类型的核酸。 特别地，提供一种非常有效的分析方法，特别是对于非翻译的RNA和微RNA，其靶核酸的类型为10000或更低。 本发明通过以下步骤能够以单分子灵敏度和分辨率的高全面性和定量性能方便快速地分析核酸：一次一个分子制备一组靶核酸片段，并与核酸分子杂交 已知碱基序列并已用荧光物质标记，与靶核酸片段组一起检测标记杂交核酸分子的荧光物质。
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