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    NUCLEIC ACID SYNTHESIS TECHNIQUES
    1.
    发明公开
    NUCLEIC ACID SYNTHESIS TECHNIQUES 审中-公开
    NUKLEINSÄURESYNTHESETECHNIKEN

    公开(公告)号：EP3143161A1

    公开(公告)日：2017-03-22

    申请号：EP15729968.6

    申请日：2015-05-14

    申请人： Illumina, Inc.

    发明人： RONAGHI, Mostafa ,  HE, Molly ,  CHEN, Cheng-yao ,  PREVITE, Michael ,  BOWEN, Shane

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6874 ,  B01J19/0046 ,  B01J2219/00587 ,  B01J2219/00605 ,  B01J2219/00689 ,  B01J2219/00693 ,  B01J2219/00695 ,  B01J2219/00698 ,  B01J2219/00704 ,  B01J2219/00722 ,  C12Q2525/191 ,  C12Q2533/101 ,  C12Q2565/501 ,  C12Q2565/543

    摘要： A method for synthesizing a nucleic acid includes synthesizing one or more nucleic acid fragments on a substrate. The synthesized one or more nucleic acid fragments may be amplified on the substrate. The method also includes sequencing the synthesized or amplified one or more nucleic acid fragments on the substrate. The sequencing may provide feedback to designs of the one or more nucleic acid fragments. The method further includes harvesting the synthesized or amplified one or more nucleic acid fragments based on sequencing. The synthesized or amplified one or more nucleic acid fragments may be assembled to generate a target nucleic acid.

    摘要翻译： 合成核酸的方法包括在底物上合成一个或多个核酸片段。 合成的一个或多个核酸片段可以在底物上扩增。 该方法还包括对底物上合成或扩增的一个或多个核酸片段进行测序。 测序可以提供反馈到一个或多个核酸片段的设计。 该方法还包括基于测序收获合成的或扩增的一个或多个核酸片段。 可以组合合成或扩增的一个或多个核酸片段以产生靶核酸。

    METHODS AND SYSTEMS FOR ANALYSIS OF SEQUENCING DATA
    3.
    发明公开
    METHODS AND SYSTEMS FOR ANALYSIS OF SEQUENCING DATA 审中-公开
    方法和系统用于分析数据SEQUENCING

    公开(公告)号：EP2359289A2

    公开(公告)日：2011-08-24

    申请号：EP09829767.4

    申请日：2009-11-24

    申请人： Illumina, Inc.

    发明人： RONAGHI, Mostafa ,  ELTOUKHY, Helmy, A.

    IPC分类号： G06F19/00

    CPC分类号： G06F19/22 ,  G06F19/14 ,  G06F19/28

    摘要： The present technology relates to the methods and systems for analysis of sequencing data. In particular, methods and systems for characterizing a target nucleic acid while determining the nucleotide sequence of the target nucleic acid are described. Certain embodiments include methods and systems for identifying the source of a target nucleic acid by comparing the accumulating nucleotide sequence of a target nucleic acid to a population of reference nucleotide sequences.

    SPATIAL INDEXING OF GENETIC MATERIAL AND LIBRARY PREPARATION USING HYDROGEL BEADS AND FLOW CELLS
    6.
    发明公开
    SPATIAL INDEXING OF GENETIC MATERIAL AND LIBRARY PREPARATION USING HYDROGEL BEADS AND FLOW CELLS 审中-公开

    公开(公告)号：EP4289967A2

    公开(公告)日：2023-12-13

    申请号：EP23181622.4

    申请日：2018-07-31

    申请人： Illumina, Inc.

    发明人： KHURANA, Tarun, Kumar ,  WU, Yir-Shyuan ,  CHEN, Xi-Jun ,  GORPE-YASAR, Filiz ,  LIN, Yan-You ,  POPIC, Victoria ,  JAEGER, Erich, B. ,  RONAGHI, Mostafa

    IPC分类号： C12Q1/6869

    摘要： Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.