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    NUCLEIC ACID ANALYSIS
    1.
    发明公开
    NUCLEIC ACID ANALYSIS 审中-公开
    核酸分析

    公开(公告)号：EP3189158A1

    公开(公告)日：2017-07-12

    申请号：EP15777986.9

    申请日：2015-09-04

    申请人： REVOLUGEN LIMITED

    发明人： MINTER, Stephen, John ,  PATSOS, Georgios

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6823 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6897 ,  C12Q2521/319 ,  C12Q2537/155 ,  C12Q2563/107 ,  C12Q2565/1015

    摘要： A method of analysing for a single stranded nucleic acid present, or potentially present, in a sample comprises a step in which the target nucleic acid (if present in the sample) displaces—and hybridises to—a reporter strand originally present in an interrogating duplex structure comprised of the reporter strand and a displaceable shorter strand. The reporter strand is tagged at or adjacent one end thereof with a reporter moiety capable of providing a detectable signal. The reporter/target duplex structure is such that the reporter strand may be selectively enzymatically digested (e.g. by means of λ-exonuclease) from its end opposite the reporter moiety to release that moiety for direct or indirect detection and regenerate single stranded target, which may then cycle through a plurality of the displacement and digestion steps to result in amplification of signal.

    摘要翻译： 分析样品中存在或可能存在的单链核酸的方法包括以下步骤，其中靶核酸（如果存在于样品中）替代并杂交至最初存在于询问双链体中的报道链 结构由报道链和可移位的较短链组成。 报道链在其一端或其附近被标记有能够提供可检测信号的报道分子部分。 报道子/靶双链体结构使得报道链可以从其报道部分对面的末端选择性酶促消化（例如借助于λ-外切核酸酶）以释放该部分以用于直接或间接检测并且再生单链靶，其可以 然后循环经过多个置换和消化步骤以导致信号的放大。

    Amplification method for polynucleotide assays
    9.
    发明公开
    Amplification method for polynucleotide assays 失效
    聚核苷酸测定法的放大方法多核苷酸测定的放大方法

    公开(公告)号：EP0300796A3

    公开(公告)日：1989-07-19

    申请号：EP88306717.5

    申请日：1988-07-21

    申请人： SYNTEX (U.S.A.) INC.

    发明人： Becker, Martin ,  Goodman, Thomas ,  Rose, Samual ,  Ullman, Edwin F.

    IPC分类号： C12Q1/68 ,  C07H21/00 ,  C12P19/34

    CPC分类号： C12Q1/701 ,  C12Q1/6813 ,  C12Q1/683 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/6858 ,  Y10T436/143333 ,  C12Q2565/537 ,  C12Q2537/155 ,  C12Q2521/301

    摘要： A method is disclosed for producing multiple copies of a primary polynucleotide sequence located at the 3′ terminus of a polynucleotide. The method comprises (a) forming in the presence of nucleoside triphosphates and template-dependent polynucleotide polymerase an extension of a primary polynucleotide sequence hybridized with a template sequence of a single stranded pattern polynucleotide comprising two or more template sequences each containing one or more cleavable sites (b) cleaving into fragments said extension at cleavable sites in the presence of means for specifically cleaving said cleavable sites when said extension is hybridized with said template sequence, (c) dissociating said fragments, (d) hybridizing said fragments with single stranded pattern polynucleotide, and repeating steps (a)-(d). Steps (a)-(d) may be conducted simultaneously or wholly or partially sequentially. The method may be applied in the detection of a polynucleotide analyte in a sample suspected of containing such analyte to facilitate such detection. Also disclosed are compositions for conducting the method of the invention.