公开(公告)号：EP1212085A2

公开(公告)日：2002-06-12

申请号：EP00956004.6

申请日：2000-08-28

申请人： Inex Pharmaceuticals Corp.

发明人： SEMPLE, Sean, C. ,  HARASYM, Troy, O. ,  KLIMUK, Sandra, K. ,  KOJIC, Ljiljiana, D. ,  BRAMSON, Jonathan, L. ,  MUI, Barbara ,  HOPE, Michael, J.

IPC分类号： A61K39/39 ,  A61K31/7088 ,  A61K9/127 ,  A61P37/04 ,  A61K35/00

CPC分类号： A61K31/7088 ,  A61K9/1272 ,  A61K31/711 ,  A61K31/7125 ,  A61K39/39 ,  A61K2039/55555 ,  A61K2039/55561 ,  C12N2310/18

摘要： Lipid-nucleic acid particles can provide therapeutic benefits, even when the nucleic acid is not complementary to coding sequences in target cells. It has been found that lipid-nucleic acid particles, including those containing non-sequence specific oligodeoxynucleotides, can be used to stimulate cytokine secretion, thus enhancing the overall immune response of a treated mammal. Further, immune response to specific target antigens can be induced by administration of an antigenic molecule in association with lipid particles containing non-sequence specific oligodeoxynucleotides. The nucleic acid which is included in the lipid-nucleic acid particle can be a phosphodiester (i.e., an oligodeoxynucleotide consisting of nucleotide residues joined by phosphodiester linkages) or a modified nucleic acid which includes phosphorothioate or other modified linkages, and may suitably be one which is non-complementary to the human genome, such that it acts to provide immunostimulation in a manner which is independent of conventional base-pairing interactions between the nucleic acid and nucleic acids of the treated mammal. In particular, the nucleic acid may suitably contain an immune-stimulating motif such as a CpG motif, or an immune stimulating palindromic sequence. The cationic lipid included in the nucleic acid particles may be suitably selected from among DODAP, DODMA, DMDMA, DOTAP, DC-Chol, DDAB, DODAC, DMRIE, DOSPA and DOGS. In addition, the lipid particle may suitably contain a modified aggregation-limiting lipid such as a PEG-lipid, a PAO-lipid or a ganglioside.

公开(公告)号：EP1299085B1

公开(公告)日：2005-11-16

申请号：EP01949169.5

申请日：2001-06-29

申请人： INEX Pharmaceuticals Corp.

发明人： MADDEN, Thomas, D. ,  SEMPLE, Sean, C.

IPC分类号： A61K9/127 ,  A61K31/47 ,  A61K31/4745

CPC分类号： A61K9/127 ,  A61K9/1272 ,  A61K9/1278 ,  A61K31/4745 ,  A61K45/06 ,  A61K2300/00

公开(公告)号：EP1212085B1

公开(公告)日：2007-10-31

申请号：EP00956004.6

申请日：2000-08-28

申请人： INEX Pharmaceuticals Corp.

发明人： SEMPLE, Sean, C. ,  HARASYM, Troy, O. ,  KLIMUK, Sandra, K. ,  KOJIC, Ljiljiana, D. ,  BRAMSON, Jonathan, L. ,  MUI, Barbara ,  HOPE, Michael, J.

IPC分类号： A61K39/39 ,  A61K31/7088 ,  A61K9/127 ,  A61P37/04 ,  A61K35/00

CPC分类号： A61K31/7088 ,  A61K9/1272 ,  A61K31/711 ,  A61K31/7125 ,  A61K39/39 ,  A61K2039/55555 ,  A61K2039/55561 ,  C12N2310/18

公开(公告)号：EP1299084A2

公开(公告)日：2003-04-09

申请号：EP01949141.4

申请日：2001-06-29

申请人： Inex Pharmaceuticals Corp.

发明人： MADDEN, Thomas, D. ,  SEMPLE, Sean, C. ,  AHKONG, Quet, F.

IPC分类号： A61K9/127 ,  A61K31/47

CPC分类号： A61K9/127 ,  A61K9/1272 ,  A61K9/1278 ,  A61K31/4745 ,  A61K45/06 ,  A61K2300/00

摘要： This invention relates to liposomal antineoplastic agents (e.g., camptothecin) compositions and methods of using such compositions for treating neoplasia and for inhibiting angiogenesis. The compositions and methods are useful for modulating the plasma circulation half-life of an active agent.

公开(公告)号：EP1299085A2

公开(公告)日：2003-04-09

申请号：EP01949169.5

申请日：2001-06-29

申请人： Inex Pharmaceuticals Corp.

发明人： MADDEN, Thomas, D. ,  SEMPLE, Sean, C.

IPC分类号： A61K9/127 ,  A61K31/47 ,  A61K31/4745

CPC分类号： A61K9/127 ,  A61K9/1272 ,  A61K9/1278 ,  A61K31/4745 ,  A61K45/06 ,  A61K2300/00

摘要： This invention relates to improved liposomal camptothecin compositions and methods of manufacturing and using such compositions for treating neoplasia and for inhibiting angiogenesis.

公开(公告)号：EP1027033A2

公开(公告)日：2000-08-16

申请号：EP98921310.3

申请日：1998-05-14

申请人： Inex Pharmaceuticals Corp.

发明人： SEMPLE, Sean, C. ,  KLIMUK, Sandra, K. ,  HARASYM, Troy ,  HOPE, Michael, J. ,  ANSELL, Steven, M. ,  CULLIS, Pieter ,  SCHERRER, Peter ,  DEBEYER, Dan

IPC分类号： A61K9/00

CPC分类号： A61K9/1277 ,  A61K9/1272 ,  Y10T428/2984

摘要： Methods for the preparation of a lipid-nucleic acid composition are provided. According to the methods, a mixture of lipids containing a protonatable or deprotonatable lipid, for example an amino lipid and a lipid such as a PEG- or polyamide oligomer-modified lipid is combined with a buffered aqueous solution of a charged therapeutic agent, for example polyanionic nucleic acids, to produce particles in which the therapeutic agent is encapsulated in a lipid vesicle. Surface charges on the lipid particles are at least partially neutralized to provide surface-neutralized lipid-encapsulated compositions of the therapeutic agents. The method permits the preparation of compositions with high ratios of therapeutic agent to lipid and with encapsulation efficiencies in excess of 50 %.