公开(公告)号：EP1373534B1

公开(公告)日：2005-12-21

申请号：EP02727959.5

申请日：2002-03-07

申请人： Biogemma ,  INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA)

发明人： MARION, Didier ,  GAUTIER, Marie-Françoise ,  JOUDRIER, Philippe ,  MESSAGER, Arnaud ,  BARSBY, Tina ,  FREEMAN, Judy

IPC分类号： C12N15/82

CPC分类号： C07K14/415 ,  C12N15/8245

摘要： A new method for increasing expression of a puroindoline protein in a plant, said puroindoline protein modifying grain hardness, notably breadmaking quality, said method comprising the steps of: (e) preparing a DNA construct which will allow the expression of a puroindoline protein, (f) introducing said DNA construct into plant cell, plant tissue, or plant, (g) selecting the plant cell, or plant tissue, or plant which stably maintain the puroindoline expression, (h) and optionally regenerating fertile stable transformed plant.

公开(公告)号：EP1373534A2

公开(公告)日：2004-01-02

申请号：EP02727959.5

申请日：2002-03-07

申请人： Biogemma ,  Institut National de la Recherche Agronomique (INRA)

发明人： MARION, Didier ,  GAUTIER, Marie-Françoise ,  JOUDRIER, Philippe ,  MESSAGER, Arnaud ,  BARSBY, Tina ,  FREEMAN, Judy

IPC分类号： C12N15/82

CPC分类号： C07K14/415 ,  C12N15/8245

摘要： A new method for increasing expression of a puroindoline protein in a plant, said puroindoline protein modifying grain hardness, notably breadmaking quality, said method comprising the steps of: (e) preparing a DNA construct which will allow the expression of a puroindoline protein, (f) introducing said DNA construct into plant cell, plant tissue, or plant, (g) selecting the plant cell, or plant tissue, or plant which stably maintain the puroindoline expression, (h) and optionally regenerating fertile stable transformed plant.

公开(公告)号：EP0644898A1

公开(公告)日：1995-03-29

申请号：EP94906260.0

申请日：1994-02-10

申请人： INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA)

发明人： RECORD, Eric ,  LESAGE, Laurence ,  MARION, Didier ,  CAHAGNIER, Bernard ,  RICHARD-MOLARD, Daniel ,  ASTHER, Marcel

IPC分类号： A61K8 ,  A61K38 ,  A61P3 ,  C07K1 ,  C07K14

CPC分类号： C07K14/37 ,  A61K38/00 ,  Y10S435/911 ,  Y10S435/913 ,  Y10S435/939 ,  Y10S530/824

摘要： A family of lipid binding and transporting proteins, a method for preparing same from fungi, and their use in cosmeticology, the agri-foodstuffs industry and pharmacology, are disclosed. Said proteins are capable of binding, transporting and/or rearranging lipids between membranes, optionally in combination with active principles. Furthermore, the proteins are hydrophobic and acidic, have a molecular weight of under 50 kDa, and may be prepared from a non-toxic filamentous fungus capable of developing on a lipid-enriched medium, particularly from raw extracts of said fungi.