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    METHODS FOR ENHANCING NUCLEIC ACID HYBRIDIZATION
    5.
    发明公开
    METHODS FOR ENHANCING NUCLEIC ACID HYBRIDIZATION 有权
    增强核酸杂交的方法

    公开(公告)号：EP2553123A1

    公开(公告)日：2013-02-06

    申请号：EP11760380.3

    申请日：2011-03-28

    申请人： Integrated DNA Technologies, Inc.

    发明人： ROSE, Scott ,  BEHLKE, Mark A. ,  OWCZARZY, Richard ,  WALDER, Joseph A. ,  THOMAS, Derek M. ,  MARVIN, Michael R.

    IPC分类号： C12Q1/68 ,  A61K31/11

    CPC分类号： C12Q1/6832 ,  C07H21/04 ,  C12Q1/6818 ,  C12Q1/6851 ,  G01N33/5308 ,  G01N33/542 ,  C12Q2525/185 ,  C12Q2525/197 ,  C12Q2565/101

    摘要： A composition comprising an oligonucleotide having the structure 5′-Y1-L1-X-L2-Y2-3′. Y1 comprises a sequence of one or more DNA or RNA nucleotides, including a first nucleotide N1 having a 3′ phosphate covalently linked to L1. Y2 comprises a sequence of one or more DNA or RNA nucleotides, including a second nucleotide N2 having a 5′ phosphate covalently linked to L2. L1 and L2 each independently are a direct bond or a C1-C7 alkyl, alkynyl, alkenyl, heteroalkyl, substituted alkyl, aryl, heteroaryl, substituted aryl, cycloalkyl, alkylaryl, or alkoxyl group. X is R1 is a hydrogen or a C1-C8 alkyl. M is a label or ligand comprising a fused polycyclic aromatic moiety.

    摘要翻译： 包含具有结构5'-Y1-L1-X-L2-Y2-3'的寡核苷酸的组合物。 Y1包含一个或多个DNA或RNA核苷酸的序列，包括具有3'端的第一核苷酸N1， 磷酸与L1共价连接。 Y2包含一个或多个DNA或RNA核苷酸的序列，包括具有5'端的第二核苷酸N2， 磷酸酯与L2共价连接。 L 1和L 2各自独立地为直接键或C 1 -C 7烷基，炔基，烯基，杂烷基，取代的烷基，芳基，杂芳基，取代的芳基，环烷基，烷基芳基或烷氧基。 X是R 1是氢或C 1 -C 8烷基。 M是包含稠合多环芳族部分的标记或配体。

    METHODS AND SYSTEMS FOR ESTIMATING THE MELTING TEMPERATURE ( i T /i sb i M /i /sb ) FOR POLYNUCLEOTIDE MOLECULES
    8.
    发明公开
    METHODS AND SYSTEMS FOR ESTIMATING THE MELTING TEMPERATURE ( i T /i sb i M /i /sb ) FOR POLYNUCLEOTIDE MOLECULES 有权
    METHOD AND SYSTEM FOR FOR多核苷酸估算的熔融温度（T M）

    公开(公告)号：EP1543438A2

    公开(公告)日：2005-06-22

    申请号：EP03754518.3

    申请日：2003-09-12

    申请人： INTEGRATED DNA TECHNOLOGIES, INC.

    发明人： OWCZARZY, Richard ,  WALDER, Joseph, Alan ,  HUANG, Lingyan ,  BEHLKE, Mark, Aaron

    IPC分类号： G06F17/00 ,  G06F17/10 ,  G06F17/11 ,  C12Q1/68

    CPC分类号： G06F19/20 ,  C12Q1/6813 ,  G06F19/22

    摘要： The invention relates to methods and systems for predicting or estimating the melting temperature of duplex nucleic acids, particularly duplexes of oligonucleotides which may be used, for example, as primers or probes in PCR and/or hybridization assays. The invention also relates to methods and systems for designing and selecting oligonucleotide probes and primers having a predicted melting temperature which is optimized for such assays. To this end, algorithms and methods are provided for predicting the melting temperature of a nucleic acid having a predetermined sequence. These methods and algorithms estimate the melting temperature of a nucleic acid duplex under particular salt conditions. The methods and algorithms use novel formulas, having terms and coefficients that are functions of the particular nucleotide sequence, to estimate the effect of particular salt conditions on the melting temperature. As such, the methods and systems of the invention provide superior result compared to existing methods, which do not consider sequence dependent effects of changing salt conditions. ­