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    MULTIPLE DISPLACEMENT AMPLIFICATION
    1.
    发明公开
    MULTIPLE DISPLACEMENT AMPLIFICATION 有权
    多重置换扩增

    公开(公告)号：EP2957641A1

    公开(公告)日：2015-12-23

    申请号：EP15167348.0

    申请日：2010-10-15

    申请人： Ibis Biosciences, Inc.

    发明人： Eshoo, Mark W. ,  Picuri, John ,  Phillipson, Curtis

    IPC分类号： C12P19/34

    CPC分类号： C12P19/34 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2531/119 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2527/127 ,  C12Q2531/113

    摘要： The present invention provides methods kits and systems for performing multiple displacement amplification reactions. In one method a sample of nucleic acid is provided. The nucleic acid is contacted with a reaction mixture which includes a set of oligonucleotide primers, a one or more polymerase enzymes and a detergent. The reaction mixture is then subjected to conditions under which the nucleic acid sequence is amplified to produce an amplified product in a multiple displacement reaction. The method may also be carried out by contacting the nucleic acid with the reaction mixture in the form of an emulsion. A kit is also provided for carrying out either the methods described above. The kit includes one or more polymerases, a plurality of primers and a detergent. The kit may also include a hydrophobic polymer and may include instructions for performing a multiple displacement amplification reaction on a nucleic acid sample.

    MULTIPLE DISPLACEMENT AMPLIFICATION
    2.
    发明授权
    MULTIPLE DISPLACEMENT AMPLIFICATION 有权
    多位移AMPLIFIKATION

    公开(公告)号：EP2957641B1

    公开(公告)日：2017-05-17

    申请号：EP15167348.0

    申请日：2010-10-15

    申请人： Ibis Biosciences, Inc.

    发明人： Eshoo, Mark W. ,  Picuri, John ,  Phillipson, Curtis

    IPC分类号： C12P19/34

    CPC分类号： C12P19/34 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2531/119 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2527/127 ,  C12Q2531/113

    摘要： The present invention provides methods kits and systems for performing multiple displacement amplification reactions. In one method a sample of nucleic acid is provided. The nucleic acid is contacted with a reaction mixture which includes a set of oligonucleotide primers, a one or more polymerase enzymes and a detergent. The reaction mixture is then subjected to conditions under which the nucleic acid sequence is amplified to produce an amplified product in a multiple displacement reaction. The method may also be carried out by contacting the nucleic acid with the reaction mixture in the form of an emulsion. A kit is also provided for carrying out either the methods described above. The kit includes one or more polymerases, a plurality of primers and a detergent. The kit may also include a hydrophobic polymer and may include instructions for performing a multiple displacement amplification reaction on a nucleic acid sample.

    摘要翻译： 本发明提供了用于进行多重置换扩增反应的方法试剂盒和系统。 在一种方法中，提供了核酸样品。 核酸与包括一组寡核苷酸引物，一种或多种聚合酶和洗涤剂的反应混合物接触。 然后将反应混合物进行条件，在该条件下扩增核酸序列以在多重置换反应中产生扩增产物。 该方法还可以通过使核酸与乳液形式的反应混合物接触来进行。 还提供了用于执行上述方法的套件。 试剂盒包括一种或多种聚合酶，多种引物和洗涤剂。 试剂盒还可以包括疏水性聚合物，并且可以包括在核酸样品上进行多重置换扩增反应的说明书。