公开(公告)号：EP3038834A1

公开(公告)日：2016-07-06

申请号：EP14767207.5

申请日：2014-08-29

申请人： Illumina, Inc. ,  Advanced Liquid Logic France S.A.S.

发明人： JAMSHIDI, Arash ,  LIN, Yan-You ,  ARAVANIS, Alex ,  DELATTRE, Cyril ,  RIVAL, Arnaud ,  FOLEY, Jennifer ,  SABOUNCHI, Poorya ,  KHURANA, Tarun ,  BABAZADEH, Majid ,  AMINI, Hamed ,  VENKATESAN, Bala Murali ,  BOWEN, M. Shane ,  BARNARD, Steven M. ,  ROGERT BACIGALUPO, Maria Candelaria ,  DEHLINGER, Dietrich

IPC分类号： B41J2/04

CPC分类号： B01L3/50273 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/087 ,  B01L2300/161 ,  B01L2300/166 ,  B01L2400/0427 ,  B41J2/04 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/1006

摘要： Provided herein is a droplet actuator including (a) first and second substrates separated by a droplet-operations gap, the first and second substrates including respective hydrophobic surfaces that face the droplet-operations gap; (b) a plurality of electrodes coupled to at least one of the first substrate and the second substrate, the electrodes arranged along the droplet-operations gap to control movement of a droplet along the hydrophobic surfaces within the droplet-operations gap; and (c) a hydrophilic or variegated-hydrophilic surface exposed to the droplet-operations gap, the hydrophilic or variegated-hydrophilic surface being positioned to contact the droplet when the droplet is at a select position within the droplet-operations gap.

摘要翻译： 本文提供的液滴致动器包括（a）由液滴操作间隙分开的第一和第二基板，第一和第二基板包括面对液滴操作间隙的各个疏水表面; （b）耦合到所述第一基板和所述第二基板中的至少一个的多个电极，所述电极沿着所述液滴操作间隙布置以控制液滴沿着所述液滴操作间隙内的疏水表面的运动; 和（c）暴露于液滴操作间隙的亲水或杂色亲水表面，当液滴在液滴操作间隙内的选择位置时，亲水或杂色亲水表面定位成接触液滴。

公开(公告)号：EP3918048A1

公开(公告)日：2021-12-08

申请号：EP20747859.5

申请日：2020-01-23

申请人： Illumina, Inc.

发明人： KRAFT, Lewis J. ,  KHURANA, Tarun Kumar ,  WU, Yir-Shyuan ,  CHEN, Xi-Jun ,  RIVAL, Arnaud ,  FULLERTON, Justin ,  BOWEN, M. Shane ,  HAN, Hui ,  FISHER, Jeffrey S. ,  FARSHCHI, Yasaman ,  LESSARD-VIGER, Mathieu

IPC分类号： C12M1/00 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N37/00

公开(公告)号：EP3727674A1

公开(公告)日：2020-10-28

申请号：EP18892345.2

申请日：2018-12-17

申请人： Illumina Inc. ,  Illumina Cambridge Limited

发明人： REN, Hongji ,  BOUTELL, Jonathan Mark ,  MOON, John A. ,  BOWEN, M. Shane ,  NEMIROSKI, Alex ,  SKINNER, Gary Mark ,  CHEN, Kenny

IPC分类号： B01J19/00 ,  C12Q1/6874 ,  C08F220/58 ,  C09D105/12 ,  C09D133/26 ,  C09D171/02

公开(公告)号：EP3577440A1

公开(公告)日：2019-12-11

申请号：EP18747994.4

申请日：2018-01-31

申请人： Illumina, Inc.

发明人： VIECELI, John S. ,  NEMIROSKI, Alex ,  BELITZ, Paul ,  LANGLOIS, Robert ,  BOWEN, M. Shane ,  CHAN, Danny Yuan ,  VENKATESAN, Bala Murali K. ,  HAN, Hui ,  SAMIEE, Kevan ,  TANNER, Stephen

IPC分类号： G01N21/64

公开(公告)号：EP3084523A1

公开(公告)日：2016-10-26

申请号：EP14824694.5

申请日：2014-12-17

申请人： Illumina, Inc.

发明人： BOWEN, M. Shane ,  VENKATESAN, Bala Murali ,  BARNARD, Steven M.

IPC分类号： G03F7/00

CPC分类号： G03F7/0002 ,  B29C59/005 ,  B29C59/022 ,  B29K2077/00 ,  B29K2105/0085 ,  C08G69/00

摘要： Substrates comprising a functionalizable layer, a polymer layer comprising a plurality of micro-scale or nano-scale patterns, or combinations thereof, and a backing layer and the preparation thereof by using room-temperature UV nano-embossing processes are disclosed. The substrates can be prepared by a roll-to-roll continuous process. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.

公开(公告)号：EP3775847A1

公开(公告)日：2021-02-17

申请号：EP19776155.4

申请日：2019-03-12

申请人： Illumina, Inc.

发明人： SUN, Yinghua ,  HONG, Stanley S. ,  ERIE, Frederick ,  NEMIROSKI, Alex ,  BOWEN, M. Shane ,  CONDELLO, Danilo ,  DEHLINGER, Dietrich ,  KRUMBUEGEL, Marco A. ,  LAM, Anthony ,  LIU, Aaron ,  OBRADOVIC, Bojan ,  PRATT, Mark

IPC分类号： G01N21/17 ,  G01N21/01

公开(公告)号：EP3577442A1

公开(公告)日：2019-12-11

申请号：EP18748211.2

申请日：2018-01-31

申请人： Illumina, Inc.

发明人： VIECELI, John S. ,  NEMIROSKI, Alex ,  BELITZ, Paul ,  LANGLOIS, Robert ,  BOWEN, M. Shane ,  CHAN, Danny Yuan ,  VENKATESAN, Bala Murali K. ,  HAN, Hui ,  SAMIEE, Kevan ,  TANNER, Stephen

IPC分类号： G01N21/64

公开(公告)号：EP3519894A1

公开(公告)日：2019-08-07

申请号：EP17857180.8

申请日：2017-09-11

申请人： Illumina, Inc.

发明人： HAN, Hui ,  YUAN, Dajun ,  BOWEN, M. Shane

IPC分类号： G03F7/00 ,  H01L23/00 ,  H01L21/033

公开(公告)号：EP2861756B1

公开(公告)日：2018-01-10

申请号：EP13731229.4

申请日：2013-06-12

申请人： Illumina, Inc.

发明人： SHEN, Min-Jui Richard ,  BOUTELL, Jonathan Mark ,  STEPHENS, Kathryn M. ,  RONAGHI, Mostafa ,  GUNDERSON, Kevin L. ,  VENKATESAN, Bala Murali ,  BOWEN, M. Shane ,  VIJAYAN, Kandaswamy

IPC分类号： C12Q1/68 ,  G01N27/447

CPC分类号： C12Q1/6848 ,  B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00675 ,  B01J2219/00722 ,  C12Q1/6844 ,  C12Q1/6874 ,  C12Q2521/507 ,  C12Q2527/101 ,  C12Q2527/113 ,  C12Q2535/122 ,  C12Q2565/513 ,  C12Q2565/537

摘要： A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.

公开(公告)号：EP2961524A1

公开(公告)日：2016-01-06

申请号：EP14710688.4

申请日：2014-02-21

申请人： Illumina, Inc.

发明人： BARNARD, Steven M. ,  BOWEN, M. Shane ,  ROGERT BACIGALUPO, Maria Candelaria ,  GEORGE, Wayne N. ,  BROWN, Andrew A. ,  TSAY, James

IPC分类号： B01J19/00 ,  C12Q1/68 ,  G01N27/447

CPC分类号： B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00621 ,  B01J2219/00644 ,  B01J2219/00722 ,  C12N15/1068 ,  C12Q1/6837 ,  C12Q2563/159

摘要： Provided is an array including a solid support having a surface, the surface having a plurality of wells, the wells containing a gel material, the wells being separated from each other by interstitial regions on the surface, the interstitial regions segregating the gel material in each of the wells from the gel material in other wells of the plurality; and a library of target nucleic acids in the gel material, wherein the gel material in each of the wells comprises a single species of the target nucleic acids of the library. Methods for making and using the array are also provided.

摘要翻译： 提供了一种包括具有表面的固体支持物的表面，表面具有多个孔，孔包含凝胶材料，孔通过表面上的间隙区彼此分离，间隙区域在每个孔中分离凝胶材料 的来自多个其他孔中的凝胶材料的孔; 以及凝胶材料中的靶核酸文库，其中每个孔中的凝胶材料包含文库的单个靶核酸的种类。 还提供了制作和使用阵列的方法。