公开(公告)号：EP3038834A1

公开(公告)日：2016-07-06

申请号：EP14767207.5

申请日：2014-08-29

申请人： Illumina, Inc. ,  Advanced Liquid Logic France S.A.S.

发明人： JAMSHIDI, Arash ,  LIN, Yan-You ,  ARAVANIS, Alex ,  DELATTRE, Cyril ,  RIVAL, Arnaud ,  FOLEY, Jennifer ,  SABOUNCHI, Poorya ,  KHURANA, Tarun ,  BABAZADEH, Majid ,  AMINI, Hamed ,  VENKATESAN, Bala Murali ,  BOWEN, M. Shane ,  BARNARD, Steven M. ,  ROGERT BACIGALUPO, Maria Candelaria ,  DEHLINGER, Dietrich

IPC分类号： B41J2/04

CPC分类号： B01L3/50273 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/087 ,  B01L2300/161 ,  B01L2300/166 ,  B01L2400/0427 ,  B41J2/04 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/1006

摘要： Provided herein is a droplet actuator including (a) first and second substrates separated by a droplet-operations gap, the first and second substrates including respective hydrophobic surfaces that face the droplet-operations gap; (b) a plurality of electrodes coupled to at least one of the first substrate and the second substrate, the electrodes arranged along the droplet-operations gap to control movement of a droplet along the hydrophobic surfaces within the droplet-operations gap; and (c) a hydrophilic or variegated-hydrophilic surface exposed to the droplet-operations gap, the hydrophilic or variegated-hydrophilic surface being positioned to contact the droplet when the droplet is at a select position within the droplet-operations gap.

摘要翻译： 本文提供的液滴致动器包括（a）由液滴操作间隙分开的第一和第二基板，第一和第二基板包括面对液滴操作间隙的各个疏水表面; （b）耦合到所述第一基板和所述第二基板中的至少一个的多个电极，所述电极沿着所述液滴操作间隙布置以控制液滴沿着所述液滴操作间隙内的疏水表面的运动; 和（c）暴露于液滴操作间隙的亲水或杂色亲水表面，当液滴在液滴操作间隙内的选择位置时，亲水或杂色亲水表面定位成接触液滴。

公开(公告)号：EP3374083A1

公开(公告)日：2018-09-19

申请号：EP16805610.9

申请日：2016-11-09

申请人： Illumina, Inc.

发明人： AMINI, Hamed ,  JAMSHIDI, Arash ,  KHURANA, Tarun, Kumar ,  MASHAYEKHI, Foad ,  WU, Yir-Shyuan

IPC分类号： B01L3/00 ,  B01J19/00

CPC分类号： B01L3/502761 ,  B01F3/0807 ,  B01F13/0062 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00722 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0858 ,  B01L2300/0867 ,  B01L2300/0883

摘要： Described are microfluidic devices and methods for providing a predetermined number of microspheres or beads, together with a cell, within a fluid droplet being processed. The system may provide each droplet with a single bead and a single cell, and the bead may contain DNA or other reagents for later identifying the specific cell associated with that bead.

公开(公告)号：EP3341494A1

公开(公告)日：2018-07-04

申请号：EP16760336.4

申请日：2016-08-26

申请人： Illumina, Inc.

发明人： SALATHIA, Neeraj ,  FAN, Jian-Bing ,  KAPER, Fiona ,  CANN, Gordon M. ,  JAMSHIDI, Arash ,  ARAVANIS, Alex

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/6806 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q2525/191 ,  C12Q2563/149 ,  C12Q2563/179 ,  C12Q2565/514

摘要： Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and/or beads bearing unique barcodes such as unique molecular barcodes (UMI).

公开(公告)号：EP4086357A1

公开(公告)日：2022-11-09

申请号：EP22175902.0

申请日：2016-08-26

申请人： Illumina, Inc.

发明人： SALATHIA, Neeraj ,  FAN, Jian-Bing ,  KAPER, Fiona ,  CANN, Gordon M. ,  JAMSHIDI, Arash ,  ARAVANIS, Alex

IPC分类号： C12Q1/68 ,  C12N15/10

摘要： Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and/or beads bearing unique barcodes such as unique molecular barcodes (UMI).

公开(公告)号：EP2776165A2

公开(公告)日：2014-09-17

申请号：EP12795930.2

申请日：2012-11-06

申请人： Illumina, Inc.

发明人： SHEN, Min-Jui Richard ,  KAIN, Robert C. ,  KUHN, Kenneth M. ,  TALASAZ, AmirAli Hajhossein ,  JAMSHIDI, Arash ,  SAKALDASIS, George ,  VERMAAS, Eric ,  BOHM, Sebastian ,  KHURANA, Tarun ,  ELTOUKHY, Helmy A. ,  GONG, Jian

IPC分类号： B01L3/00

CPC分类号： C12Q1/6874 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0851 ,  B01L2300/165 ,  B01L2400/0427

摘要： Provided are methods and apparatuses for performing sequencing using droplet manipulation, for example, via electrowetting-based techniques. Also provided are integrated methods and apparatuses for performing sample preparation and sequencing on the same apparatus. In addition, provided are methods of reducing reagent waste and preloaded consumable cartridges comprising reagents for sample preparation and/or sequencing.

摘要翻译： 提供了用于使用液滴操纵进行排序的方法和装置，例如通过基于电润湿的技术。 还提供了用于在相同装置上进行样品制备和测序的综合方法和装置。 此外，提供了减少试剂废物的方法和包含用于样品制备和/或测序的试剂的预装消耗筒。

公开(公告)号：EP3680333A1

公开(公告)日：2020-07-15

申请号：EP20154378.2

申请日：2015-04-28

申请人： Illumina, Inc.

发明人： KAPER, Fiona ,  FAN, Jian-Bing ,  SALATHIA, Neeraj ,  CANN, Gordon ,  JAMSHIDI, Arash ,  ARAVANIS, Alex

IPC分类号： C12N15/10

摘要： Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and/or beads bearing unique barcodes such as unique molecular barcodes (UMI).

公开(公告)号：EP3384046A1

公开(公告)日：2018-10-10

申请号：EP16816512.4

申请日：2016-11-30

申请人： Illumina, Inc.

发明人： JAMSHIDI, Arash ,  LIN, Yan-you ,  ABSALAN, Farnaz ,  STUART, Sarah ,  CANN, Gordon ,  WU, Yir-Shyuan ,  KHURANA, Tarun ,  FISHER, Jeffrey, S.

IPC分类号： C12Q1/68 ,  G01N1/28 ,  B01L3/00 ,  G01N15/10

CPC分类号： B01L3/502761 ,  B01L3/502784 ,  B01L3/5088 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/165 ,  B01L2400/0427 ,  B01L2400/043 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2563/185 ,  G01N1/28 ,  G01N33/54333 ,  G01N2015/1006 ,  C12Q2535/122

摘要： In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.

公开(公告)号：EP3907295A1

公开(公告)日：2021-11-10

申请号：EP21170333.5

申请日：2016-11-30

申请人： Illumina, Inc.

发明人： JAMSHIDI, Arash ,  LIN, Yan-you ,  ABSALAN, Farnaz ,  STUART, Sarah ,  CANN, Gordon ,  WU, Yir-Shyuan ,  KHURANA, Tarun ,  FISHER, Jeffrey S.

IPC分类号： C12Q1/68 ,  G01N1/28 ,  B01L3/00 ,  G01N15/10

摘要： In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.

公开(公告)号：EP3137601B1

公开(公告)日：2020-04-08

申请号：EP15721102.0

申请日：2015-04-28

申请人： Illumina, Inc.

发明人： KAPER, Fiona ,  FAN, Jian-Bing ,  SALATHIA, Neeraj ,  CANN, Gordon M. ,  JAMSHIDI, Arash ,  ARAVANIS, Alex

IPC分类号： C12N15/10

公开(公告)号：EP3038834B1

公开(公告)日：2018-12-12

申请号：EP14767207.5

申请日：2014-08-29

申请人： Illumina, Inc. ,  Illumina France S.A.R.L.

发明人： JAMSHIDI, Arash ,  LIN, Yan-You ,  ARAVANIS, Alex ,  DELATTRE, Cyril ,  RIVAL, Arnaud ,  FOLEY, Jennifer ,  SABOUNCHI, Poorya ,  KHURANA, Tarun ,  BABAZADEH, Majid ,  AMINI, Hamed ,  VENKATESAN, Bala Murali ,  BOWEN, M. Shane ,  BARNARD, Steven M. ,  ROGERT BACIGALUPO, Maria Candelaria ,  DEHLINGER, Dietrich

IPC分类号： B41J2/04

CPC分类号： B01L3/50273 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/087 ,  B01L2300/161 ,  B01L2300/166 ,  B01L2400/0427 ,  B41J2/04 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/1006

摘要： Provided herein is a droplet actuator including (a) first and second substrates separated by a droplet-operations gap, the first and second substrates including respective hydrophobic surfaces that face the droplet-operations gap; (b) a plurality of electrodes coupled to at least one of the first substrate and the second substrate, the electrodes arranged along the droplet-operations gap to control movement of a droplet along the hydrophobic surfaces within the droplet-operations gap; and (c) a hydrophilic or variegated-hydrophilic surface exposed to the droplet-operations gap, the hydrophilic or variegated-hydrophilic surface being positioned to contact the droplet when the droplet is at a select position within the droplet-operations gap.