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    PYROPHOSPHOROLYTIC SEQUENCING
    1.
    发明公开
    PYROPHOSPHOROLYTIC SEQUENCING 有权
    磷酸二苯酯顺式麻醉剂NANOPOREN

    公开(公告)号：EP3004378A1

    公开(公告)日：2016-04-13

    申请号：EP14725498.1

    申请日：2014-05-22

    申请人： Illumina Cambridge Limited

    发明人： MEULEMAN, Wouter

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2521/319 ,  C12Q2521/525 ,  C12Q2565/631

    摘要： A method for determining the sequence of a target nucleic acid, including steps of contacting a target nucleic acid with a polymerase to sequentially remove nucleotide triphosphates from the target nucleic acid, wherein the nucleotide triphosphates that are removed have a variety of different base moieties; and distinguishing the different base moieties for the nucleotide triphosphates that are removed. Also provided is a apparatus including a nanopore positioned in a fluid impermeable barrier to form a passage through which a nucleotide triphosphate can pass from a first fluid reservoir to a second fluid reservoir, and a reaction mix in the first fluid reservoir that includes a polymerase, target nucleic acid having two strands, and pyrophosphoro lytic concentration of pyrophosphate.

    摘要翻译： 一种用于确定靶核酸序列的方法，包括使目标核酸与聚合酶接触以顺序地从靶核酸中除去核苷酸三磷酸的步骤，其中被去除的三磷酸核苷酸具有多种不同的碱基部分; 并区分去除的三磷酸核苷酸的不同碱基部分。 还提供了一种装置，其包括定位在流体不可渗透屏障中的纳米孔，以形成三磷酸核苷从第一流体储存器传递到第二流体储存器的通道，以及包括聚合酶的第一流体储存器中的反应混合物， 具有两条链的靶核酸和焦磷酸焦磷酸浓度。

    PYROPHOSPHOROLYTIC SEQUENCING USING NANOPORES
    2.
    发明公开
    PYROPHOSPHOROLYTIC SEQUENCING USING NANOPORES 审中-公开

    公开(公告)号：EP3825415A1

    公开(公告)日：2021-05-26

    申请号：EP20183774.7

    申请日：2014-05-22

    申请人： Illumina Cambridge Limited

    发明人： MEULEMAN, Wouter

    IPC分类号： C12Q1/6869

    摘要： A method for determining the sequence of a target nucleic acid, including steps of contacting a target nucleic acid with a polymerase which is associated with a nanopore to sequentially remove nucleotide triphosphates from the target nucleic acid by pyrophosphorolysis; and distinguishing the different base moieties for the nucleotide triphosphates that are removed wherein the base moieties are distinguished by a change in current in the nanopore.

    PYROPHOSPHOROLYTIC SEQUENCING USING NANOPORES
    8.
    发明公开
    PYROPHOSPHOROLYTIC SEQUENCING USING NANOPORES 审中-公开

    公开(公告)号：EP3351645A1

    公开(公告)日：2018-07-25

    申请号：EP17207528.5

    申请日：2014-05-22

    申请人： Illumina Cambridge Limited

    发明人： MEULEMAN, Wouter

    IPC分类号： C12Q1/6869

    摘要： A method for determining the sequence of a target nucleic acid, including steps of contacting a target nucleic acid with a polymerase to sequentially remove nucleotide triphosphates from the target nucleic acid, wherein the nucleotide triphosphates that are removed have a variety of different base moieties; and distinguishing the different base moieties for the nucleotide triphosphates that are removed. Also provided is an apparatus including a nanopore positioned in a fluid impermeable barrier to form a passage through which a nucleotide triphosphate can pass from a first fluid reservoir to a second fluid reservoir, and a reaction mix in the first fluid reservoir that includes a polymerase, target nucleic acid having two strands, and pyrophosphorolytic concentration of pyrophosphate.