METHODS FOR PAIRED-END SEQUENCING OF POLYNUCLEOTIDES
    4.
    发明公开
    METHODS FOR PAIRED-END SEQUENCING OF POLYNUCLEOTIDES 审中-公开
    用于多核苷酸的成对序列分析的方法

    公开(公告)号:EP3205730A1

    公开(公告)日:2017-08-16

    申请号:EP17161616.2

    申请日:2011-08-29

    IPC分类号: C12Q1/68

    摘要: Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first portion of each polynucleotide molecule is attached to a first location of the surface and a second portion of each polynucleotide molecule is attached to a second location of the surface, the relative proximity of the first and second locations being correlated with the probability that the first and second portions are paired, separating the first and second portions of the polynucleotide molecules on the surface, determining the sequences of the first and second portions of the polynucleotide molecules and comparing the relative proximities and the sequences to determine which first and second portions are paired and to determine the sequence of the target polynucleotide molecules.

    摘要翻译: 本文提供了用于测序多核苷酸分子的方法。 该方法包括以下步骤:提供附着于表面的多个多核苷酸分子,其中每个多核苷酸分子的第一部分连接至表面的第一位置,并且每个多核苷酸分子的第二部分连接至表面的第二位置 表面,第一和第二位置的相对接近度与第一和第二部分配对的概率相关,分离表面上的多核苷酸分子的第一和第二部分,确定第一和第二部分的第一和第二部分的序列 多核苷酸分子,并比较相对邻近性和序列以确定哪一个第一和第二部分配对并确定靶多核苷酸分子的序列。

    IMPROVED METHODS OF NUCLEIC ACID SEQUENCING
    6.
    发明公开
    IMPROVED METHODS OF NUCLEIC ACID SEQUENCING 有权
    改进的核酸测序方法

    公开(公告)号:EP2823058A1

    公开(公告)日:2015-01-14

    申请号:EP13714865.6

    申请日:2013-03-06

    IPC分类号: C12Q1/68

    摘要: Provided herein is a method of using transposition to improve methods of sequencing RNA molecules. Provided herein is a method of tagging nucleic acid duplexes, such as DNA:RNA duplexes or DNA:DNA duplexes. The method includes the steps of providing a transposase and a transposon composition, providing one or more nucleic acid duplexes immobilized on a support, and contacting the transposase and transposon composition with the one or more nucleic acid duplexes under conditions wherein the one or more nucleic acid duplexes and transposon composition undergo a transposition reaction to produce one or more tagged nucleic acid duplexes, wherein the transposon composition comprises a double stranded nucleic acid molecule comprising a transferred strand and a non-transferred strand.

    摘要翻译: 本文提供了使用转座来改进RNA分子测序方法的方法。 本文提供了标记核酸双链体的方法,例如DNA:RNA双链体或DNA:DNA双链体。 该方法包括以下步骤:提供转座酶和转座子组合物,提供固定在支持物上的一种或多种核酸双链体,以及在一种或多种核酸在一种或多种核酸条件下与一种或多种核酸双链体接触转座酶和转座子组合物 双链体和转座子组合物经历转位反应以产生一个或多个标记的核酸双链体,其中所述转座子组合物包含含有转移链和非转移链的双链核酸分子。