公开(公告)号：EP3615922A1

公开(公告)日：2020-03-04

申请号：EP18791184.7

申请日：2018-04-19

申请人： Illumina Inc.

发明人： FUNG, Tracy Helen ,  CAI, Xiuyu ,  KWOK, Lisa ,  TRAN, Hai ,  SAMIEE, Kevan ,  QIANG, Liangliang ,  BOYANOV, Boyan

IPC分类号： G01N21/78 ,  G01N21/77 ,  G01N35/00

公开(公告)号：EP3854884A1

公开(公告)日：2021-07-28

申请号：EP21160000.2

申请日：2016-08-12

申请人： Illumina, Inc.

发明人： MANDELL, Jeffrey G. ,  KWOK, Lisa

IPC分类号： C12Q1/6869 ,  G01R33/09 ,  G01N33/543 ,  G01N27/00 ,  B01L3/00

摘要： Sequencing-by-synthesis (SBS) method is provided that includes providing a detection apparatus that includes an array of magnetically-responsive sensors. Each of the magnetically-responsive sensors is located proximate to a respective designated space to detect a magnetic property therefrom. The detection apparatus also includes a plurality of nucleic acid template strands located within corresponding designated spaces. The method also includes conducting a plurality of SBS events to grow a complementary strand by incorporating nucleotides along each template strand. At least some of the nucleotides are attached to corresponding magnetic particles having respective magnetic properties. Each of the plurality of SBS events includes detecting changes in electrical resistance at the magnetically-responsive sensors caused by the respective magnetic properties of the magnetic particles. The method also includes determining genetic characteristics of the complementary strands based on the detected changes in electrical resistance.

公开(公告)号：EP4075135A1

公开(公告)日：2022-10-19

申请号：EP22177086.0

申请日：2018-04-19

申请人： Illumina, Inc.

发明人： FUNG, Tracy Helen ,  CAI, Xiuyu ,  KWOK, Lisa ,  TRAN, Hai ,  SAMIEE, Kevan ,  QIANG, Liangliang ,  BOYANOV, Boyan

IPC分类号： G01N33/543 ,  C12Q1/68 ,  G01N21/62 ,  G01N21/64 ,  G01N21/77 ,  B01L3/00

摘要： An example sensor includes a detection device, including an optical waveguide, an optical sensor operatively associated with the optical waveguide and a device circuitry, including a reagent electrode, a first embedded metal layer electrically connected to the reagent electrode; and a second embedded metal layer electrically connected to the optical sensor wherein the first embedded metal layer is spaced from the second embedded metal layer by an electrically isolating gap, at least a portion of a passivation layer being in contact with the first embedded metal layer and an input region of the optical waveguide, the at least the portion of the passivation layer having a reaction site at least partially adjacent to the input region of the optical waveguide; and a lid operatively connected to the passivation layer to partially define a flow channel between the lid and the reaction site, wherein the reagent electrode is positioned to be in contact with a reagent to be introduced into the flow channel. A method for performing a sensing operation of a sensor in response to a reaction at the reaction site involving at least some reaction component of the reagent and during the sensing operation, applying an electrical bias that renders the reagent electrode one of an anode or a cathode and the embedded metal layer the other of the cathode or the anode, thereby providing cathodic protection or anodic protection to the embedded metal layer, is also disclosed.

公开(公告)号：EP3334839A1

公开(公告)日：2018-06-20

申请号：EP16757119.9

申请日：2016-08-12

申请人： Illumina, Inc.

发明人： MANDELL, Jeffrey G. ,  KWOK, Lisa

IPC分类号： C12Q1/68 ,  G01R33/09 ,  G01N33/543 ,  G01N27/00

CPC分类号： C12Q1/6874 ,  B01L3/502761 ,  C12Q1/6869 ,  C12Q2563/143 ,  C12Q2565/607 ,  G01N27/3276 ,  G01N33/543 ,  G01R33/093 ,  G01R33/1276

摘要： Sequencing-by-synthesis (SBS) method is provided that includes providing a detection apparatus that includes an array of magnetically-responsive sensors. Each of the magnetically-responsive sensors is located proximate to a respective designated space to detect a magnetic property therefrom. The detection apparatus also includes a plurality of nucleic acid template strands located within corresponding designated spaces. The method also includes conducting a plurality of SBS events to grow a complementary strand by incorporating nucleotides along each template strand. At least some of the nucleotides are attached to corresponding magnetic particles having respective magnetic properties. Each of the plurality of SBS events includes detecting changes in electrical resistance at the magnetically-responsive sensors caused by the respective magnetic properties of the magnetic particles. The method also includes determining genetic characteristics of the complementary strands based on the detected changes in electrical resistance.